Restriction map for
pF1KA0404
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# NO CUT ENZYMES ( 49 enzymes) #
##################################
AclI AcsI AflII ApoI AscI
AseI AsnI AvrII BamHI BclI
BfrI BlnI BsiWI BspDI BspHI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EcoRI EcoRV
FbaI FseI HpaI MfeI MluI
MunI NdeI NheI NotI NruI
NspV PacI PmeI PshBI Psp1406I
RcaI RsrII SalI SfuI SspI
SwaI TfiI Tsp509I XbaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1320 GACGTC GACGT^C
Acc65I 2893 GGTACC G^GTACC
AccIII 1336 TCCGGA T^CCGGA
AgeI 5405 ACCGGT A^CCGGT
Asp700I 3180 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2893 GGTACC G^GTACC
AspI 517 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3655 CACGTG CAC^GTG
BciVI 5589 GGATAC GTATCC(6/5)
BglII 2541 AGATCT A^GATCT
BseAI 1336 TCCGGA T^CCGGA
BsmI 3255 GCATTC GAATGC(1/-1)
Bsp1407I 125 TGTACA T^GTACA
BspEI 1336 TCCGGA T^CCGGA
BspLU11I 3131 ACATGT A^CATGT
BsrGI 125 TGTACA T^GTACA
BstAPI 669 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3081 GGTGACC G^GT[ACGT]ACC
BstPI 3081 GGTGACC G^GT[ACGT]ACC
EcoO65I 3081 GGTGACC G^GT[ACGT]ACC
EcoT22I 369 ATGCAT ATGCA^T
HindIII 4467 AAGCTT A^AGCTT
KpnI 2893 GGTACC GGTAC^C
KspI 3404 CCGCGG CCGC^GG
MroI 1336 TCCGGA T^CCGGA
NsiI 369 ATGCAT ATGCA^T
PaeR7I 4871 CTCGAG C^TCGAG
PflFI 517 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5405 ACCGGT A^CCGGT
PmaCI 3655 CACGTG CAC^GTG
PmlI 3655 CACGTG CAC^GTG
PshAI 4541 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4196 CGATCG CGAT^CG
SacII 3404 CCGCGG CCGC^GG
SbfI 5484 CCTGCAGG CCTGCA^GG
SexAI 5060 ACCAGGT A^CC[AT]GGT
SgrAI 635 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 3706 TACGTA TAC^GTA
SpeI 4804 ACTAGT A^CTAGT
SphI 5741 GCATGC GCATG^C
Sse8387I 5484 CCTGCAGG CCTGCA^GG
SspBI 125 TGTACA T^GTACA
Tth111I 517 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4871 CTCGAG C^TCGAG
XmnI 3180 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AflIII 3131 ACATGT A^C[GA][CT]GT
3654 ACACGT
AhdI 1506 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5449 GACATCCTGTC
Aor51HI 4683 AGCGCT AGC^GCT
5495 AGCGCT
AspEI 1506 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5449 GACATCCTGTC
BbeI 630 GGCGCC GGCGC^C
5650 GGCGCC
BbsI 973 GAAGAC GAAGAC(2/6)
2756 GTCTTC
BcgI 1371 CGAGTTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3672 CGACTCCTGTGC
BpuAI 973 GAAGAC GAAGAC(2/6)
2756 GTCTTC
BsaBI 643 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2364 GATGGTGATC
BsrDI 1735 CATTGC GCAATG(2/0)
1944 CATTGC
DraIII 2238 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4477 CACGAGGTG
EagI 2984 CGGCCG C^GGCCG
4192 CGGCCG
Eam1105I 1506 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5449 GACATCCTGTC
EclXI 2984 CGGCCG C^GGCCG
4192 CGGCCG
Eco47III 4683 AGCGCT AGC^GCT
5495 AGCGCT
Eco52I 2984 CGGCCG C^GGCCG
4192 CGGCCG
HincII 3556 GTTGAC GT[CT]^[GA]AC
3691 GTCAAC
HindII 3556 GTTGAC GT[CT]^[GA]AC
3691 GTCAAC
KasI 630 GGCGCC G^GCGCC
5650 GGCGCC
MamI 643 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2364 GATGGTGATC
MseI 2618 TTAA T^TAA
3578 TTAA
NarI 630 GGCGCC GG^CGCC
5650 GGCGCC
SapI 727 GAAGAGC GCTCTTC(1/4)
5268 GCTCTTC
SfiI 3103 GGCCGCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5709 GGCCACGGAGGCC
SfoI 630 GGCGCC GGC^GCC
5650 GGCGCC
SmaI 1583 CCCGGG CCC^GGG
1916 CCCGGG
Tru9I 2618 TTAA T^TAA
3578 TTAA
XmaCI 1583 CCCGGG C^CCGGG
1916 CCCGGG
XmaI 1583 CCCGGG C^CCGGG
1916 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
AviII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII