Restriction map for
pF1KA0405
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AvaI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglII BlnI BsaI BsaWI BseAI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
KasI KpnI KspI MluI MluNI
MroI MscI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I StuI SwaI
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1596 GACGTC GACGT^C
AcyI 1596 GACGTC G[GA]^CG[CT]C
Alw44I 268 GTGCAC G^TGCAC
ApaLI 268 GTGCAC G^TGCAC
AspI 1593 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1021 GGTCC G^G[AT]CC
BclI 761 TGATCA T^GATCA
BglI 522 GCCTGTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1674 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1674 GCTCAGC GC^T[ACGT]AGC
BsaAI 713 TACGTA [CT]AC^GT[GA]
BsaBI 562 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1596 GACGTC G[GA]^CG[CT]C
BseRI 734 CTCCTC GAGGAG(10/8)
BsmAI 1828 GTCTC GTCTC(1/5)
BsmBI 1827 CGTCTC CGTCTC(1/5)
Bsp1407I 258 TGTACA T^GTACA
BsrFI 1745 GCCGGC [GA]^CCGG[CT]
BsrGI 258 TGTACA T^GTACA
BssSI 609 CACGAG CACGAG(-5/-1)
BstXI 92 CCAAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1674 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1745 GCCGGC [GA]^CCGG[CT]
DraIII 15 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 30 CCATGG C^C[GA][CT]GG
EaeI 1043 TGGCCG [CT]^GGCC[GA]
EcoRI 304 GAATTC G^AATTC
FbaI 761 TGATCA T^GATCA
HaeII 1709 GGCGCT [GA]GCGC^[CT]
Hin1I 1596 GACGTC G[GA]^CG[CT]C
HincII 1441 GTTAAC GT[CT]^[GA]AC
HindII 1441 GTTAAC GT[CT]^[GA]AC
HpaI 1441 GTTAAC GTT^AAC
MamI 562 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 707 CAATTG C^AATTG
MunI 707 CAATTG C^AATTG
NaeI 1745 GCCGGC GCC^GGC
NcoI 30 CCATGG C^CATGG
NdeI 1693 CATATG CA^TATG
NgoMIV 1745 GCCGGC G^CCGGC
NspI 1931 ACATGC [GA]CATG^[CT]
PflFI 1593 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 873 GACTC GAGTC(4/5)
PpuMI 1020 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1317 CAGCTG CAG^CTG
SapI 406 GAAGAGC GCTCTTC(1/4)
SnaBI 713 TACGTA TAC^GTA
SspBI 258 TGTACA T^GTACA
SspI 358 AATATT AAT^ATT
Tth111I 1593 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 139 GTCTAC GT^[AC][GT]AC
277 GTCTAC
AcsI 304 GAATTC [GA]^AATT[CT]
700 GAATTT
ApoI 304 GAATTC [GA]^AATT[CT]
700 GAATTT
AseI 223 ATTAAT AT^TAAT
1900 ATTAAT
AsnI 223 ATTAAT AT^TAAT
1900 ATTAAT
AspHI 268 GTGCAC G[AT]GC[AT]^C
1966 GAGCAC
BamHI 179 GGATCC G^GATCC
1271 GGATCC
BanI 670 GGCACC G^G[CT][GA]CC
1774 GGCACC
BbsI 451 GAAGAC GAAGAC(2/6)
1513 GAAGAC
BfaI 110 CTAG C^TAG
1172 CTAG
BpmI 1798 CTGGAG CTGGAG(16/14)
1963 CTGGAG
BpuAI 451 GAAGAC GAAGAC(2/6)
1513 GAAGAC
BsgI 250 CTGCAC GTGCAG(16/14)
412 CTGCAC
BsiHKAI 268 GTGCAC G[AT]GC[AT]^C
1966 GAGCAC
BstYI 179 GGATCC [GA]^GATC[CT]
1271 GGATCC
DraII 457 GGGGCCT [GA]G^G[ACGT]CC[CT]
1020 AGGTCCT
EcoO109I 457 GGGGCCT [GA]G^G[ACGT]CC[CT]
1020 AGGTCCT
FauI 1002 GCGGG CCCGC(4/6)
1630 GCGGG
HindIII 156 AAGCTT A^AGCTT
400 AAGCTT
MaeI 110 CTAG C^TAG
1172 CTAG
MflI 179 GGATCC [GA]^GATC[CT]
1271 GGATCC
PflMI 14 CCACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1602 CCACAGCATGG
PshBI 223 ATTAAT AT^TAAT
1900 ATTAAT
SmlI 694 CTCAAG C^T[CT][GA]AG
875 CTCAAG
TaqI 570 TCGA T^CGA
1207 TCGA
TthHB8I 570 TCGA T^CGA
1207 TCGA
Van91I 14 CCACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1602 CCACAGCATGG
XcmI 431 CCATATCCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
437 CCACAGTGGGGGTGG
XhoII 179 GGATCC [GA]^GATC[CT]
1271 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI