Restriction map for
pF1KA0424
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BfrI BglI BlnI BsaAI BsaBI
BsiWI BsmBI BsoBI Bsp1407I BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI Bsu36I Cfr10I
CpoI DraIII Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoT22I FseI
FspI HaeII KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 97 GACGTC GACGT^C
AccI 774 GTATAC GT^[AC][GT]AC
AccIII 1064 TCCGGA T^CCGGA
AcsI 1307 AAATTT [GA]^AATT[CT]
AcyI 97 GACGTC G[GA]^CG[CT]C
AflIII 413 ACATGT A^C[GA][CT]GT
AlwNI 1435 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 223 GGGCCC GGGCC^C
ApoI 1307 AAATTT [GA]^AATT[CT]
BalI 65 TGGCCA TGG^CCA
BbsI 1015 GTCTTC GAAGAC(2/6)
BciVI 744 GTATCC GTATCC(6/5)
BclI 11 TGATCA T^GATCA
BglII 734 AGATCT A^GATCT
BlpI 760 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 760 GCTGAGC GC^T[ACGT]AGC
BpuAI 1015 GTCTTC GAAGAC(2/6)
BsaHI 97 GACGTC G[GA]^CG[CT]C
BsaI 491 GAGACC GGTCTC(1/5)
BsaWI 1064 TCCGGA [AT]^CCGG[AT]
BseAI 1064 TCCGGA T^CCGGA
BsiEI 996 CGGCCG CG[GA][CT]^CG
BsmI 1167 GAATGC GAATGC(1/-1)
BspDI 703 ATCGAT AT^CGAT
BspEI 1064 TCCGGA T^CCGGA
BspHI 827 TCATGA T^CATGA
BspLU11I 413 ACATGT A^CATGT
BsrBI 1383 CCGCTC CCGCTC(-3/-3)
Bst1107I 774 GTATAC GTA^TAC
BstXI 1220 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 774 GTATAC GTA^TAC
CelII 760 GCTGAGC GC^T[ACGT]AGC
CfoI 1494 GCGC GCG^C
ClaI 703 ATCGAT AT^CGAT
DraI 85 TTTAAA TTT^AAA
DrdI 1147 GACTTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 996 CGGCCG C^GGCCG
EclXI 996 CGGCCG C^GGCCG
Eco52I 996 CGGCCG C^GGCCG
EcoRV 460 GATATC GAT^ATC
EcoT14I 62 CCATGG C^C[AT][AT]GG
FbaI 11 TGATCA T^GATCA
HgaI 345 GCGTC GACGC(5/10)
HhaI 1494 GCGC GCG^C
Hin1I 97 GACGTC G[GA]^CG[CT]C
HinP1I 1494 GCGC G^CGC
HindIII 1177 AAGCTT A^AGCTT
HpaI 1530 GTTAAC GTT^AAC
MluNI 65 TGGCCA TGG^CCA
MroI 1064 TCCGGA T^CCGGA
MscI 65 TGGCCA TGG^CCA
NcoI 62 CCATGG C^CATGG
NspI 413 ACATGT [GA]CATG^[CT]
PvuII 812 CAGCTG CAG^CTG
RcaI 827 TCATGA T^CATGA
SexAI 1439 ACCTGGT A^CC[AT]GGT
SmlI 364 CTCAAG C^T[CT][GA]AG
SspI 877 AATATT AAT^ATT
StuI 665 AGGCCT AGG^CCT
StyI 62 CCATGG C^C[AT][AT]GG
XcmI 68 CCAACCGGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 51 GGATC GGATC(4/5)
980 GGATC
BbvCI 528 CCTCAGC CCTCAGC(-5/-2)
37 GCTGAGG
BcnI 72 CCGGG CC^[GC]GG
297 CCGGG
BsaJI 62 CCATGG C^C[ACGT][ACGT]GG
1428 CCACGG
BsgI 235 GTGCAG GTGCAG(16/14)
727 GTGCAG
BspMI 1467 GCAGGT ACCTGC(4/8)
1526 GCAGGT
BsrDI 696 CATTGC GCAATG(2/0)
1336 GCAATG
BstYI 734 AGATCT [GA]^GATC[CT]
980 GGATCT
DsaI 62 CCATGG C^C[GA][CT]GG
1428 CCACGG
MflI 734 AGATCT [GA]^GATC[CT]
980 GGATCT
MspA1I 812 CAGCTG C[AC]G^C[GT]G
1009 CAGCGG
MwoI 82 GCATTTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
814 GCTGCTTTGGC
NciI 72 CCGGG CC^[GC]GG
297 CCGGG
PflMI 1032 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1434 CCAGTACCTGG
PleI 720 GACTC GAGTC(4/5)
840 GACTC
TaqI 149 TCGA T^CGA
704 TCGA
TfiI 22 GATTC G^A[AT]TC
569 GATTC
TthHB8I 149 TCGA T^CGA
704 TCGA
Van91I 1032 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1434 CCAGTACCTGG
XhoII 734 AGATCT [GA]^GATC[CT]
980 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BfaI BmyI BpmI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI NdeII NlaIII NlaIV
PpuMI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI Tru9I TseI
Tsp45I Tsp509I TspRI