Restriction map for
pF1KA0428
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwI AocI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BlnI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
HphI KasI KpnI Ksp632I KspI
MamI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Alw44I 970 GTGCAC G^TGCAC
ApaLI 970 GTGCAC G^TGCAC
AseI 525 ATTAAT AT^TAAT
AsnI 525 ATTAAT AT^TAAT
AspHI 970 GTGCAC G[AT]GC[AT]^C
AvaI 689 CTCGGG C^[CT]CG[GA]G
BalI 278 TGGCCA TGG^CCA
BcgI 109 GCACATCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 280 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 443 AGATCT A^GATCT
BlpI 368 GCTTAGC GC^T[ACGT]AGC
BpmI 183 CTCCAG CTGGAG(16/14)
Bpu1102I 368 GCTTAGC GC^T[ACGT]AGC
BseRI 584 GAGGAG GAGGAG(10/8)
BsiHKAI 970 GTGCAC G[AT]GC[AT]^C
BsoBI 689 CTCGGG C^[CT]CG[GA]G
BspMI 495 ACCTGC ACCTGC(4/8)
BstBI 117 TTCGAA TT^CGAA
BstYI 443 AGATCT [GA]^GATC[CT]
CelII 368 GCTTAGC GC^T[ACGT]AGC
DdeI 369 CTTAG C^T[ACGT]AG
DraI 222 TTTAAA TTT^AAA
DraII 411 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 654 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 643 GACAACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 411 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 809 GAATTC G^AATTC
EcoT22I 380 ATGCAT ATGCA^T
HgaI 248 GACGC GACGC(5/10)
HincII 760 GTCAAC GT[CT]^[GA]AC
HindII 760 GTCAAC GT[CT]^[GA]AC
HindIII 366 AAGCTT A^AGCTT
MaeII 567 ACGT A^CGT
MfeI 573 CAATTG C^AATTG
MflI 443 AGATCT [GA]^GATC[CT]
MluNI 278 TGGCCA TGG^CCA
MscI 278 TGGCCA TGG^CCA
MunI 573 CAATTG C^AATTG
NsiI 380 ATGCAT ATGCA^T
NspI 923 ACATGC [GA]CATG^[CT]
NspV 117 TTCGAA TT^CGAA
PpuMI 411 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 525 ATTAAT AT^TAAT
SexAI 755 ACCAGGT A^CC[AT]GGT
SfiI 279 GGCCATGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 117 TTCGAA TT^CGAA
StuI 844 AGGCCT AGG^CCT
XcmI 129 CCAAGTTGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 443 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 103 AAATTT [GA]^AATT[CT]
809 GAATTC
ApoI 103 AAATTT [GA]^AATT[CT]
809 GAATTC
AvaII 412 GGACC G^G[AT]CC
432 GGTCC
BanI 325 GGTGCC G^G[CT][GA]CC
868 GGTGCC
BcnI 435 CCCGG CC^[GC]GG
481 CCGGG
BmyI 326 GTGCCC G[AGT]GC[ACT]^C
970 GTGCAC
Bsp1286I 326 GTGCCC G[AGT]GC[ACT]^C
970 GTGCAC
DpnII 444 GATC ^GATC
738 GATC
DsaI 803 CCATGG C^C[GA][CT]GG
965 CCATGG
EaeI 85 CGGCCA [CT]^GGCC[GA]
278 TGGCCA
EcoT14I 803 CCATGG C^C[AT][AT]GG
965 CCATGG
HapII 436 CCGG C^CGG
481 CCGG
HpaII 436 CCGG C^CGG
481 CCGG
MboI 444 GATC ^GATC
738 GATC
MboII 270 GAAGA GAAGA(8/7)
204 TCTTC
MslI 276 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1090 CACAAGTATG
MspI 436 CCGG C^CGG
481 CCGG
NciI 435 CCCGG CC^[GC]GG
481 CCGGG
NcoI 803 CCATGG C^CATGG
965 CCATGG
NdeII 444 GATC ^GATC
738 GATC
Sau3AI 444 GATC ^GATC
738 GATC
SmlI 544 CTTGAG C^T[CT][GA]AG
815 CTCAAG
StyI 803 CCATGG C^C[AT][AT]GG
965 CCATGG
TaqI 118 TCGA T^CGA
555 TCGA
Tsp45I 13 GTCAC ^GT[GC]AC
652 GTCAC
TthHB8I 118 TCGA T^CGA
555 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AlwNI BbvI
BfaI BsaJI BsgI BsiYI BslI
BsmFI BsrI BssKI BstAPI BstF5I
BstNI Cac8I Cfr13I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MaeIII MnlI MseI MspA1I MvaI
MwoI NlaIII NlaIV PstI PvuII
RsaI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI