Restriction map for
pF1KA0431
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BciVI BlnI BlpI Bpu1102I
BsaBI BsaHI BseAI BsiEI BsmBI
BsmI BsoBI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StyI SwaI Tth111I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflII 616 CTTAAG C^TTAAG
AlwNI 545 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
BanII 534 GGGCTC G[GA]GC[CT]^C
BbrPI 735 CACGTG CAC^GTG
BbsI 346 GAAGAC GAAGAC(2/6)
BbvCI 500 CCTCAGC CCTCAGC(-5/-2)
BcgI 174 CGAGATACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 528 TGATCA T^GATCA
BcnI 1426 CCCGG CC^[GC]GG
BfrI 616 CTTAAG C^TTAAG
BglI 541 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 777 AGATCT A^GATCT
BpuAI 346 GAAGAC GAAGAC(2/6)
BsaAI 735 CACGTG [CT]AC^GT[GA]
BsaWI 104 TCCGGT [AT]^CCGG[AT]
BseRI 498 CTCCTC GAGGAG(10/8)
BsiWI 50 CGTACG C^GTACG
Bsp1407I 1047 TGTACA T^GTACA
BspDI 1432 ATCGAT AT^CGAT
BsrDI 1894 GCAATG GCAATG(2/0)
BsrGI 1047 TGTACA T^GTACA
BssSI 172 CACGAG CACGAG(-5/-1)
BstAPI 555 GCACTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 984 CCAGTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 777 AGATCT [GA]^GATC[CT]
ClaI 1432 ATCGAT AT^CGAT
DraI 1194 TTTAAA TTT^AAA
DrdI 774 GACAGATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 528 TGATCA T^GATCA
HindIII 314 AAGCTT A^AGCTT
MflI 777 AGATCT [GA]^GATC[CT]
NciI 1426 CCCGG CC^[GC]GG
PmaCI 735 CACGTG CAC^GTG
PmlI 735 CACGTG CAC^GTG
SphI 9 GCATGC GCATG^C
SspBI 1047 TGTACA T^GTACA
StuI 1617 AGGCCT AGG^CCT
Tsp45I 1530 GTCAC ^GT[GC]AC
XbaI 1791 TCTAGA T^CTAGA
XhoII 777 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1230 AAATTT [GA]^AATT[CT]
1602 AAATTC
AflIII 734 ACACGT A^C[GA][CT]GT
736 ACGTGT
AlwI 585 GATCC GGATC(4/5)
1353 GGATC
ApoI 1230 AAATTT [GA]^AATT[CT]
1602 AAATTC
BbvI 41 GCAGC GCAGC(8/12)
949 GCTGC
BpmI 999 CTCCAG CTGGAG(16/14)
1710 CTCCAG
BsaI 1747 GAGACC GGTCTC(1/5)
996 GGTCTC
BspMI 180 ACCTGC ACCTGC(4/8)
1785 ACCTGC
BstF5I 1038 GGATG GGATG(2/0)
1899 GGATG
DraII 65 GGGACCT [GA]G^G[ACGT]CC[CT]
194 GGGACCC
EcoO109I 65 GGGACCT [GA]G^G[ACGT]CC[CT]
194 GGGACCC
EcoRV 398 GATATC GAT^ATC
1441 GATATC
EcoT22I 7 ATGCAT ATGCA^T
962 ATGCAT
FokI 1038 GGATG GGATG(9/13)
1899 GGATG
HapII 105 CCGG C^CGG
1427 CCGG
HpaII 105 CCGG C^CGG
1427 CCGG
HphI 661 GGTGA GGTGA(8/7)
1871 TCACC
MaeII 736 ACGT A^CGT
767 ACGT
MspI 105 CCGG C^CGG
1427 CCGG
NsiI 7 ATGCAT ATGCA^T
962 ATGCAT
PflMI 507 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1344 CCAGACATTGG
PpuMI 65 GGGACCT [GA]G^G[AT]CC[CT]
194 GGGACCC
PstI 145 CTGCAG CTGCA^G
182 CTGCAG
ScaI 673 AGTACT AGT^ACT
1726 AGTACT
SfaNI 456 GATGC GCATC(5/9)
961 GATGC
TaqI 1433 TCGA T^CGA
1445 TCGA
TseI 41 GCAGC G^C[AT]GC
949 GCTGC
TthHB8I 1433 TCGA T^CGA
1445 TCGA
Van91I 507 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1344 CCAGACATTGG
XcmI 428 CCAAAGTGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1398 CCAGCTCCCATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI Alw44I ApaLI
AspHI AvaII BfaI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI HaeIII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
TfiI Tru9I Tsp509I TspRI