Restriction map for
pF1KA0437
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AclI AocI Aor51HI
ApaI AscI Asp718I AspI AviII
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FseI FspI KpnI MfeI
MluI MunI NdeI NheI NotI
NruI PacI PaeR7I PflFI PmeI
Psp1406I PvuI RsrII SalI ScaI
SexAI SfiI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 3693 GTCTAC GT^[AC][GT]AC
AccIII 1199 TCCGGA T^CCGGA
AflII 18 CTTAAG C^TTAAG
AgeI 604 ACCGGT A^CCGGT
Alw44I 1524 GTGCAC G^TGCAC
ApaLI 1524 GTGCAC G^TGCAC
AvrII 3489 CCTAGG C^CTAGG
BbeI 4554 GGCGCC GGCGC^C
BbvCI 68 CCTCAGC CCTCAGC(-5/-2)
BciVI 1349 GGATAC GTATCC(6/5)
BfrI 18 CTTAAG C^TTAAG
BlnI 3489 CCTAGG C^CTAGG
BseAI 1199 TCCGGA T^CCGGA
BspEI 1199 TCCGGA T^CCGGA
BstBI 3858 TTCGAA TT^CGAA
BstXI 2495 CCAGACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoT22I 4431 ATGCAT ATGCA^T
HaeII 4554 GGCGCC [GA]GCGC^[CT]
HindIII 3659 AAGCTT A^AGCTT
HpaI 1342 GTTAAC GTT^AAC
KasI 4554 GGCGCC G^GCGCC
KspI 1489 CCGCGG CCGC^GG
MroI 1199 TCCGGA T^CCGGA
NaeI 3649 GCCGGC GCC^GGC
NarI 4554 GGCGCC GG^CGCC
NcoI 1502 CCATGG C^CATGG
NgoMIV 3649 GCCGGC G^CCGGC
NsiI 4431 ATGCAT ATGCA^T
NspI 3380 ACATGC [GA]CATG^[CT]
NspV 3858 TTCGAA TT^CGAA
PinAI 604 ACCGGT A^CCGGT
PshAI 1186 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 83 CAGCTG CAG^CTG
SacII 1489 CCGCGG CCGC^GG
SbfI 3348 CCTGCAGG CCTGCA^GG
SfoI 4554 GGCGCC GGC^GCC
SfuI 3858 TTCGAA TT^CGAA
SgrAI 603 CACCGGTG C[GA]^CCGG[CT]G
SmaI 4547 CCCGGG CCC^GGG
Sse8387I 3348 CCTGCAGG CCTGCA^GG
XmaCI 4547 CCCGGG C^CCGGG
XmaI 4547 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 815 ACACGT A^C[GA][CT]GT
3836 ACGTGT
AhdI 3034 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3685 GACACCCTGTC
AseI 2137 ATTAAT AT^TAAT
2962 ATTAAT
AsnI 2137 ATTAAT AT^TAAT
2962 ATTAAT
Asp700I 699 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4188 GAAGAGGTTC
AspEI 3034 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3685 GACACCCTGTC
BbrPI 816 CACGTG CAC^GTG
4285 CACGTG
BcgI 4153 GCAGCAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4337 GCAACAACTTCG
BclI 1736 TGATCA T^GATCA
2970 TGATCA
BglI 1129 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4546 GCCCGGGAGGC
BglII 846 AGATCT A^GATCT
2039 AGATCT
BlpI 3319 GCTAAGC GC^T[ACGT]AGC
4681 GCTCAGC
Bpu1102I 3319 GCTAAGC GC^T[ACGT]AGC
4681 GCTCAGC
BsaAI 816 CACGTG [CT]AC^GT[GA]
4285 CACGTG
BsmBI 1795 GAGACG CGTCTC(1/5)
2587 GAGACG
BsmI 1068 GAATGC GAATGC(1/-1)
3155 GAATGC
BspMI 3347 ACCTGC ACCTGC(4/8)
4439 ACCTGC
BssSI 2932 CACGAG CACGAG(-5/-1)
3816 CACGAG
BstAPI 1120 GCAGATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1776 GCAGCCTTTGC
CelII 3319 GCTAAGC GC^T[ACGT]AGC
4681 GCTCAGC
Eam1105I 3034 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3685 GACACCCTGTC
EcoNI 588 CCTCCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4716 CCTTCCCCAGG
FbaI 1736 TGATCA T^GATCA
2970 TGATCA
HincII 1342 GTTAAC GT[CT]^[GA]AC
4099 GTTGAC
HindII 1342 GTTAAC GT[CT]^[GA]AC
4099 GTTGAC
PmaCI 816 CACGTG CAC^GTG
4285 CACGTG
PmlI 816 CACGTG CAC^GTG
4285 CACGTG
PshBI 2137 ATTAAT AT^TAAT
2962 ATTAAT
SacI 2652 GAGCTC GAGCT^C
3586 GAGCTC
StuI 2248 AGGCCT AGG^CCT
3061 AGGCCT
XmnI 699 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4188 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII