Restriction map for
pF1KA0443
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AviII BbeI BfrI BglI BglII
BsaBI BsaHI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI Bsu36I CfoI ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 2559 TCCGGA T^CCGGA
AflIII 3085 ACGTGT A^C[GA][CT]GT
ApaI 820 GGGCCC GGGCC^C
AseI 3483 ATTAAT AT^TAAT
AsnI 3483 ATTAAT AT^TAAT
AspHI 730 GAGCTC G[AT]GC[AT]^C
AvrII 532 CCTAGG C^CTAGG
BamHI 3501 GGATCC G^GATCC
BanI 25 GGTGCC G^G[CT][GA]CC
BbrPI 3084 CACGTG CAC^GTG
BcgI 1009 CGAGAAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 3753 CCCGG CC^[GC]GG
BlnI 532 CCTAGG C^CTAGG
BlpI 286 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 286 GCTCAGC GC^T[ACGT]AGC
BsaAI 3084 CACGTG [CT]AC^GT[GA]
BsaWI 2559 TCCGGA [AT]^CCGG[AT]
BseAI 2559 TCCGGA T^CCGGA
BsgI 206 GTGCAG GTGCAG(16/14)
BsiHKAI 730 GAGCTC G[AT]GC[AT]^C
BsmI 4102 GCATTC GAATGC(1/-1)
BspEI 2559 TCCGGA T^CCGGA
BsrFI 2062 GCCGGT [GA]^CCGG[CT]
Bst1107I 2657 GTATAC GTA^TAC
BstZ17I 2657 GTATAC GTA^TAC
CelII 286 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2062 GCCGGT [GA]^CCGG[CT]
DsaI 3166 CCATGG C^C[GA][CT]GG
MaeII 3085 ACGT A^CGT
MroI 2559 TCCGGA T^CCGGA
NciI 3753 CCCGG CC^[GC]GG
NcoI 3166 CCATGG C^CATGG
NdeI 3708 CATATG CA^TATG
PmaCI 3084 CACGTG CAC^GTG
PmlI 3084 CACGTG CAC^GTG
PshAI 1971 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3483 ATTAAT AT^TAAT
SacI 730 GAGCTC GAGCT^C
ScaI 367 AGTACT AGT^ACT
XbaI 2827 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 30 enzymes) #
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AciI 4090 CCGC CCGC(-3/-1)
1579 GCGG
Asp700I 3490 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3545 GAAGTGCTTC
AspI 1623 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2104 GACATTGTC
BalI 683 TGGCCA TGG^CCA
2861 TGGCCA
BbsI 904 GAAGAC GAAGAC(2/6)
2398 GAAGAC
BciVI 2055 GTATCC GTATCC(6/5)
2776 GTATCC
BclI 1109 TGATCA T^GATCA
3312 TGATCA
BpuAI 904 GAAGAC GAAGAC(2/6)
2398 GAAGAC
BspHI 3513 TCATGA T^CATGA
3923 TCATGA
BspMI 842 GCAGGT ACCTGC(4/8)
3077 GCAGGT
BstXI 674 CCATGCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3257 CCAGGAACATGG
EcoNI 402 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1094 CCTTTTCAAGG
FbaI 1109 TGATCA T^GATCA
3312 TGATCA
HincII 1522 GTCAAC GT[CT]^[GA]AC
1885 GTCAAC
HindII 1522 GTCAAC GT[CT]^[GA]AC
1885 GTCAAC
HindIII 1013 AAGCTT A^AGCTT
1865 AAGCTT
MluNI 683 TGGCCA TGG^CCA
2861 TGGCCA
MscI 683 TGGCCA TGG^CCA
2861 TGGCCA
MslI 675 CATGCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3704 CATACATATG
NheI 1351 GCTAGC G^CTAGC
2406 GCTAGC
PflFI 1623 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2104 GACATTGTC
PflMI 806 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1930 CCATGTTTTGG
RcaI 3513 TCATGA T^CATGA
3923 TCATGA
SexAI 811 ACCTGGT A^CC[AT]GGT
1219 ACCTGGT
TaqI 3375 TCGA T^CGA
3576 TCGA
Tth111I 1623 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2104 GACATTGTC
TthHB8I 3375 TCGA T^CGA
3576 TCGA
Van91I 806 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1930 CCATGTTTTGG
XcmI 132 CCAGATAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1274 CCAGTCTACAAGTGG
XmnI 3490 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3545 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
AlwNI ApoI AvaI AvaII BanII
BbvCI BbvI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII