Restriction map for
pF1KA0464
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BbeI BbrPI BbsI BcgI BclI
BcnI BfrI BlnI BpuAI BsaBI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AcsI 679 GAATTC [GA]^AATT[CT]
AcyI 70 GACGCC G[GA]^CG[CT]C
ApoI 679 GAATTC [GA]^AATT[CT]
Asp700I 363 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 383 CTCGAG C^[CT]CG[GA]G
BalI 528 TGGCCA TGG^CCA
BamHI 50 GGATCC G^GATCC
BanI 391 GGTGCC G^G[CT][GA]CC
BanII 763 GAGCCC G[GA]GC[CT]^C
BbvCI 930 CCTCAGC CCTCAGC(-5/-2)
BciVI 170 GGATAC GTATCC(6/5)
BglII 365 AGATCT A^GATCT
BlpI 495 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 495 GCTGAGC GC^T[ACGT]AGC
BsaAI 106 TACGTA [CT]AC^GT[GA]
BsaHI 70 GACGCC G[GA]^CG[CT]C
BsaI 572 GAGACC GGTCTC(1/5)
BsmFI 652 GTCCC GGGAC(10/14)
BsmI 519 GAATGC GAATGC(1/-1)
BsoBI 383 CTCGAG C^[CT]CG[GA]G
BspDI 637 ATCGAT AT^CGAT
BspHI 345 TCATGA T^CATGA
BspMI 44 ACCTGC ACCTGC(4/8)
BsrI 592 ACTGG ACTGG(1/-1)
BstAPI 39 GCACGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 495 GCTGAGC GC^T[ACGT]AGC
CfoI 165 GCGC GCG^C
ClaI 637 ATCGAT AT^CGAT
DrdI 837 GACACCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 611 CCACGG C^C[GA][CT]GG
EarI 435 GAAGAG CTCTTC(1/4)
EcoNI 779 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 679 GAATTC G^AATTC
HapII 168 CCGG C^CGG
HgaI 70 GACGC GACGC(5/10)
HhaI 165 GCGC GCG^C
Hin1I 70 GACGCC G[GA]^CG[CT]C
HinP1I 165 GCGC G^CGC
HpaII 168 CCGG C^CGG
Ksp632I 435 GAAGAG CTCTTC(1/4)
MluNI 528 TGGCCA TGG^CCA
MscI 528 TGGCCA TGG^CCA
MslI 959 CACTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 841 CCGCTG C[AC]G^C[GT]G
MspI 168 CCGG C^CGG
PaeR7I 383 CTCGAG C^TCGAG
RcaI 345 TCATGA T^CATGA
SapI 435 GAAGAGC GCTCTTC(1/4)
SfiI 606 GGCCTCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 383 CTCGAG C^T[CT][GA]AG
SnaBI 106 TACGTA TAC^GTA
Tsp45I 694 GTGAC ^GT[GC]AC
Tsp509I 680 AATT ^AATT
XbaI 702 TCTAGA T^CTAGA
XcmI 134 CCAACAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 383 CTCGAG C^TCGAG
XmnI 363 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 18 GTAC GT^AC
105 GTAC
AvaII 315 GGACC G^G[AT]CC
651 GGTCC
BfaI 5 CTAG C^TAG
703 CTAG
BglI 598 GCCGAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
607 GCCTCCACGGC
DraI 184 TTTAAA TTT^AAA
421 TTTAAA
EaeI 528 TGGCCA [CT]^GGCC[GA]
614 CGGCCA
EcoT14I 127 CCAAGG C^C[AT][AT]GG
743 CCAAGG
MaeI 5 CTAG C^TAG
703 CTAG
MaeIII 413 GTAAC ^GT[ACGT]AC
694 GTGAC
MseI 185 TTAA T^TAA
422 TTAA
PpuMI 314 AGGACCC [GA]G^G[AT]CC[CT]
650 AGGTCCC
PstI 502 CTGCAG CTGCA^G
620 CTGCAG
RsaI 18 GTAC GT^AC
105 GTAC
StuI 473 AGGCCT AGG^CCT
822 AGGCCT
StyI 127 CCAAGG C^C[AT][AT]GG
743 CCAAGG
Tru9I 185 TTAA T^TAA
422 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwI BbvI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeII MboI MboII MflI MnlI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI TseI TspRI TthHB8I
XhoII