Restriction map for
pF1KA0473
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BspHI BspLU11I BssHII
BssSI BstAPI BstUI CelII CfoI
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI FbaI FseI FspI HaeII
HhaI HinP1I HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NarI NdeI NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 2707 GAATTT [GA]^AATT[CT]
AcyI 2136 GGCGTC G[GA]^CG[CT]C
Alw44I 2626 GTGCAC G^TGCAC
AlwNI 2020 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2626 GTGCAC G^TGCAC
ApoI 2707 GAATTT [GA]^AATT[CT]
BalI 2432 TGGCCA TGG^CCA
BcnI 369 CCGGG CC^[GC]GG
BglI 390 GCCCATTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1153 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2136 GGCGTC G[GA]^CG[CT]C
BsaWI 1978 ACCGGA [AT]^CCGG[AT]
BsmI 379 GAATGC GAATGC(1/-1)
Bsp1407I 2603 TGTACA T^GTACA
BsrBI 1683 CCGCTC CCGCTC(-3/-3)
BsrFI 926 GCCGGC [GA]^CCGG[CT]
BsrGI 2603 TGTACA T^GTACA
Bst1107I 322 GTATAC GTA^TAC
BstBI 281 TTCGAA TT^CGAA
BstEII 942 GGTGACC G^GT[ACGT]ACC
BstPI 942 GGTGACC G^GT[ACGT]ACC
BstXI 2435 CCAAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 322 GTATAC GTA^TAC
Cfr10I 926 GCCGGC [GA]^CCGG[CT]
DraI 1008 TTTAAA TTT^AAA
DraII 2394 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 1129 CCATGG C^C[GA][CT]GG
EaeI 2432 TGGCCA [CT]^GGCC[GA]
EcoO109I 2394 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 942 GGTGACC G^GT[ACGT]ACC
EcoRV 1231 GATATC GAT^ATC
Hin1I 2136 GGCGTC G[GA]^CG[CT]C
HindIII 285 AAGCTT A^AGCTT
MamI 1153 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2432 TGGCCA TGG^CCA
MscI 2432 TGGCCA TGG^CCA
NaeI 926 GCCGGC GCC^GGC
NciI 369 CCGGG CC^[GC]GG
NcoI 1129 CCATGG C^CATGG
NgoMIV 926 GCCGGC G^CCGGC
NspV 281 TTCGAA TT^CGAA
PshAI 1655 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 457 CTGCAG CTGCA^G
SacI 2686 GAGCTC GAGCT^C
SexAI 2573 ACCTGGT A^CC[AT]GGT
SfuI 281 TTCGAA TT^CGAA
SmlI 906 CTTGAG C^T[CT][GA]AG
SspBI 2603 TGTACA T^GTACA
XbaI 148 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 322 GTATAC GT^[AC][GT]AC
1662 GTCTAC
AocI 1208 CCTTAGG CC^T[ACGT]AGG
1480 CCTGAGG
Asp700I 1311 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1844 GAAGTCCTTC
BbsI 1327 GAAGAC GAAGAC(2/6)
2347 GAAGAC
BbvCI 1060 CCTCAGC CCTCAGC(-5/-2)
2209 CCTCAGC
BpuAI 1327 GAAGAC GAAGAC(2/6)
2347 GAAGAC
BseRI 1547 CTCCTC GAGGAG(10/8)
2229 CTCCTC
BspMI 1656 ACCTGC ACCTGC(4/8)
2589 GCAGGT
BsrDI 1403 GCAATG GCAATG(2/0)
1516 GCAATG
Bsu36I 1208 CCTTAGG CC^T[ACGT]AGG
1480 CCTGAGG
EarI 1171 CTCTTC CTCTTC(1/4)
1176 CTCTTC
Eco81I 1208 CCTTAGG CC^T[ACGT]AGG
1480 CCTGAGG
EcoT22I 1034 ATGCAT ATGCA^T
2518 ATGCAT
FauI 2112 CCCGC CCCGC(4/6)
2159 GCGGG
Ksp632I 1171 CTCTTC CTCTTC(1/4)
1176 CTCTTC
NsiI 1034 ATGCAT ATGCA^T
2518 ATGCAT
PleI 2022 GACTC GAGTC(4/5)
1430 GAGTC
SphI 1923 GCATGC GCATG^C
2219 GCATGC
XmnI 1311 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1844 GAAGTCCTTC
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BamHI BanI BanII BbvI
BfaI BmyI BpmI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII