Restriction map for
pF1KA0519
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# NO CUT ENZYMES ( 94 enzymes) #
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AatII Acc65I AccIII AflII AflIII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BbeI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaBI BsaI BseAI
BseRI BsiEI BsiWI BspDI BspEI
BspLU11I BssHII Bst1107I BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NarI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SnaBI Sse8387I SspI StuI SwaI
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 586 GTCTAC GT^[AC][GT]AC
AccII 33 CGCG CG^CG
AclI 673 AACGTT AA^CGTT
AcyI 2134 GGCGTC G[GA]^CG[CT]C
AocI 2587 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1956 AGCGCT AGC^GCT
AspI 937 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 630 GGATCC G^GATCC
BbsI 121 GTCTTC GAAGAC(2/6)
BpuAI 121 GTCTTC GAAGAC(2/6)
BsaHI 2134 GGCGTC G[GA]^CG[CT]C
BsaWI 1130 ACCGGA [AT]^CCGG[AT]
BsgI 1156 GTGCAG GTGCAG(16/14)
BsmBI 1735 GAGACG CGTCTC(1/5)
BsmI 417 GCATTC GAATGC(1/-1)
Bsp1407I 2678 TGTACA T^GTACA
BspHI 2601 TCATGA T^CATGA
BsrGI 2678 TGTACA T^GTACA
BstAPI 375 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2550 GGTGACC G^GT[ACGT]ACC
BstPI 2550 GGTGACC G^GT[ACGT]ACC
BstUI 33 CGCG CG^CG
Bsu36I 2587 CCTCAGG CC^T[ACGT]AGG
DraIII 1238 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1956 AGCGCT AGC^GCT
Eco81I 2587 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2550 GGTGACC G^GT[ACGT]ACC
HgaI 2135 GCGTC GACGC(5/10)
Hin1I 2134 GGCGTC G[GA]^CG[CT]C
HincII 2001 GTTGAC GT[CT]^[GA]AC
HindII 2001 GTTGAC GT[CT]^[GA]AC
MseI 1580 TTAA T^TAA
MvnI 33 CGCG CG^CG
PflFI 937 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 673 AACGTT AA^CGTT
RcaI 2601 TCATGA T^CATGA
ScaI 874 AGTACT AGT^ACT
SmlI 391 CTCAAG C^T[CT][GA]AG
SpeI 720 ACTAGT A^CTAGT
SphI 56 GCATGC GCATG^C
SspBI 2678 TGTACA T^GTACA
Tru9I 1580 TTAA T^TAA
Tth111I 937 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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BbrPI 87 CACGTG CAC^GTG
232 CACGTG
BfaI 721 CTAG C^TAG
1064 CTAG
BglI 789 GCCACACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
886 GCCATGGTGGC
BlpI 93 GCTCAGC GC^T[ACGT]AGC
760 GCTGAGC
Bpu1102I 93 GCTCAGC GC^T[ACGT]AGC
760 GCTGAGC
BstBI 1087 TTCGAA TT^CGAA
1354 TTCGAA
CelII 93 GCTCAGC GC^T[ACGT]AGC
760 GCTGAGC
DrdI 604 GACCAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2128 GACATTGGCGTC
DsaI 887 CCATGG C^C[GA][CT]GG
2387 CCATGG
EaeI 879 TGGCCG [CT]^GGCC[GA]
2316 TGGCCG
EcoNI 1068 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2064 CCTGAACAAGG
EcoRI 1189 GAATTC G^AATTC
2088 GAATTC
HaeII 296 AGCGCC [GA]GCGC^[CT]
1956 AGCGCT
MaeI 721 CTAG C^TAG
1064 CTAG
NaeI 496 GCCGGC GCC^GGC
530 GCCGGC
NcoI 887 CCATGG C^CATGG
2387 CCATGG
NgoMIV 496 GCCGGC G^CCGGC
530 GCCGGC
NspV 1087 TTCGAA TT^CGAA
1354 TTCGAA
PmaCI 87 CACGTG CAC^GTG
232 CACGTG
PmlI 87 CACGTG CAC^GTG
232 CACGTG
PstI 280 CTGCAG CTGCA^G
1426 CTGCAG
SacI 274 GAGCTC GAGCT^C
430 GAGCTC
SfcI 280 CTGCAG C^T[GA][CT]AG
1426 CTGCAG
SfuI 1087 TTCGAA TT^CGAA
1354 TTCGAA
SmaI 200 CCCGGG CCC^GGG
1306 CCCGGG
XmaCI 200 CCCGGG C^CCGGG
1306 CCCGGG
XmaI 200 CCCGGG C^CCGGG
1306 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII