Restriction map for
pF1KA0523
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI AlwI Aor51HI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbeI BbsI BbvCI
BciVI BfaI BfrI BglII BlnI
BpuAI BsaBI BsaHI BsaI BsiWI
BsmBI BspDI BspLU11I BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I ClaI DraI DraIII Eam1105I
Eco47III EcoO65I EcoRV EcoT22I FseI
FspI Hin1I HpaI KasI KpnI
MaeI MamI MfeI MflI MluI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI SacI SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI Tru9I Van91I XbaI
XhoII
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# ONE CUT ENZYMES ( 67 enzymes) #
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AcsI 1312 GAATTC [GA]^AATT[CT]
Alw44I 1462 GTGCAC G^TGCAC
AocI 955 CCTGAGG CC^T[ACGT]AGG
ApaLI 1462 GTGCAC G^TGCAC
ApoI 1312 GAATTC [GA]^AATT[CT]
Asp700I 846 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1462 GTGCAC G[AT]GC[AT]^C
AspI 1384 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1092 CACGTG CAC^GTG
BclI 1643 TGATCA T^GATCA
BglI 172 GCCGCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 535 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 535 GCTGAGC GC^T[ACGT]AGC
BsaAI 1092 CACGTG [CT]AC^GT[GA]
BsgI 214 CTGCAC GTGCAG(16/14)
BsiEI 1593 CGGCCG CG[GA][CT]^CG
BsiHKAI 1462 GTGCAC G[AT]GC[AT]^C
BsmAI 511 GTCTC GTCTC(1/5)
BsmI 880 GAATGC GAATGC(1/-1)
Bsp1407I 1018 TGTACA T^GTACA
BspHI 359 TCATGA T^CATGA
BspMI 914 ACCTGC ACCTGC(4/8)
BsrGI 1018 TGTACA T^GTACA
BstAPI 156 GCAGACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1230 CCACGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 955 CCTGAGG CC^T[ACGT]AGG
CelII 535 GCTGAGC GC^T[ACGT]AGC
CpoI 730 CGGACCG CG^G[AT]CCG
DpnII 1644 GATC ^GATC
DrdI 220 GACCCTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1593 CGGCCG C^GGCCG
EclXI 1593 CGGCCG C^GGCCG
Eco52I 1593 CGGCCG C^GGCCG
Eco81I 955 CCTGAGG CC^T[ACGT]AGG
EcoNI 795 CCTGATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1312 GAATTC G^AATTC
FbaI 1643 TGATCA T^GATCA
HincII 1390 GTCAAC GT[CT]^[GA]AC
HindII 1390 GTCAAC GT[CT]^[GA]AC
HindIII 1071 AAGCTT A^AGCTT
KspI 744 CCGCGG CCGC^GG
MboI 1644 GATC ^GATC
MslI 1231 CACGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 926 CCATGG C^CATGG
NdeII 1644 GATC ^GATC
PaeR7I 224 CTCGAG C^TCGAG
PflFI 1384 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1092 CACGTG CAC^GTG
PmlI 1092 CACGTG CAC^GTG
PvuII 1349 CAGCTG CAG^CTG
RcaI 359 TCATGA T^CATGA
RsrII 730 CGGACCG CG^G[AT]CCG
SacII 744 CCGCGG CCGC^GG
SapI 1365 GAAGAGC GCTCTTC(1/4)
Sau3AI 1644 GATC ^GATC
SmaI 137 CCCGGG CCC^GGG
SphI 1116 GCATGC GCATG^C
SspBI 1018 TGTACA T^GTACA
TaqI 225 TCGA T^CGA
TfiI 1264 GATTC G^A[AT]TC
Tsp509I 1313 AATT ^AATT
Tth111I 1384 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 225 TCGA T^CGA
XhoI 224 CTCGAG C^TCGAG
XmaCI 137 CCCGGG C^CCGGG
XmaI 137 CCCGGG C^CCGGG
XmnI 846 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 550 GTCTAC GT^[AC][GT]AC
988 GTCTAC
AflIII 1091 ACACGT A^C[GA][CT]GT
1526 ACGTGT
ApaI 132 GGGCCC GGGCC^C
390 GGGCCC
BcgI 216 GCACGACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1005 GCAAGACACTCG
BpmI 22 CTCCAG CTGGAG(16/14)
717 CTCCAG
BseRI 1471 GAGGAG GAGGAG(10/8)
1540 GAGGAG
BsrBI 538 GAGCGG CCGCTC(-3/-3)
1543 GAGCGG
EarI 622 GAAGAG CTCTTC(1/4)
1365 GAAGAG
EcoT14I 857 CCTTGG C^C[AT][AT]GG
926 CCATGG
HaeII 180 GGCGCT [GA]GCGC^[CT]
1598 GGCGCT
HphI 606 GGTGA GGTGA(8/7)
1622 TCACC
Ksp632I 622 GAAGAG CTCTTC(1/4)
1365 GAAGAG
NaeI 556 GCCGGC GCC^GGC
1595 GCCGGC
NgoMIV 556 GCCGGC G^CCGGC
1595 GCCGGC
PpuMI 950 AGGACCC [GA]G^G[AT]CC[CT]
1297 AGGTCCC
StyI 857 CCTTGG C^C[AT][AT]GG
926 CCATGG
XcmI 806 CCAATGTGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1049 CCAAAGTGTTTGTGG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwNI AvaI AvaII BalI BanI
BanII BbvI BcnI BmyI BsaJI
BsaWI BseAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspEI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI MaeII MaeIII MboII MluNI
MnlI MroI MscI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV NspI PleI PstI RsaI
Sau96I ScrFI SfaNI SfcI SmlI
TseI Tsp45I TspRI