Restriction map for
pF1KA0546
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Aor51HI ApaI AscI AseI
AsnI Asp718I AviII BbeI BbrPI
BcgI BclI BglI BsaBI BsaHI
BseAI BsiWI BsmBI BspEI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I CpoI
DraIII DrdI Eco47III EcoO65I EcoRV
FbaI FseI FspI HaeII Hin1I
KasI KpnI MamI MroI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SpeI SphI Sse8387I SwaI XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 3924 GTCTAC GT^[AC][GT]AC
AflII 3172 CTTAAG C^TTAAG
AhdI 3649 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4487 GTGCAC G^TGCAC
ApaLI 4487 GTGCAC G^TGCAC
AspEI 3649 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2072 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2041 CCTAGG C^CTAGG
BanI 4211 GGTGCC G^G[CT][GA]CC
BbvCI 246 GCTGAGG CCTCAGC(-5/-2)
BfrI 3172 CTTAAG C^TTAAG
BlnI 2041 CCTAGG C^CTAGG
BlpI 2233 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2233 GCTGAGC GC^T[ACGT]AGC
BsaAI 4354 TACGTA [CT]AC^GT[GA]
CelII 2233 GCTGAGC GC^T[ACGT]AGC
CfoI 4171 GCGC GCG^C
EagI 388 CGGCCG C^GGCCG
Eam1105I 3649 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 388 CGGCCG C^GGCCG
Eco52I 388 CGGCCG C^GGCCG
EcoNI 523 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 274 GCGTC GACGC(5/10)
HhaI 4171 GCGC GCG^C
HinP1I 4171 GCGC G^CGC
KspI 157 CCGCGG CCGC^GG
MfeI 5168 CAATTG C^AATTG
MluI 272 ACGCGT A^CGCGT
MslI 4815 CACAAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 5168 CAATTG C^AATTG
NcoI 4684 CCATGG C^CATGG
PflFI 2072 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 157 CCGCGG CCGC^GG
SmaI 182 CCCGGG CCC^GGG
SnaBI 4354 TACGTA TAC^GTA
Tth111I 2072 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 182 CCCGGG C^CCGGG
XmaI 182 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AvaI 182 CCCGGG C^[CT]CG[GA]G
2753 CTCGGG
BalI 2768 TGGCCA TGG^CCA
5714 TGGCCA
BamHI 541 GGATCC G^GATCC
5392 GGATCC
BbsI 2397 GAAGAC GAAGAC(2/6)
2078 GTCTTC
BglII 1428 AGATCT A^GATCT
3133 AGATCT
BpuAI 2397 GAAGAC GAAGAC(2/6)
2078 GTCTTC
BsaI 332 GGTCTC GGTCTC(1/5)
614 GGTCTC
BsiEI 5 CGACCG CG[GA][CT]^CG
388 CGGCCG
BsoBI 182 CCCGGG C^[CT]CG[GA]G
2753 CTCGGG
Bsp1407I 1218 TGTACA T^GTACA
4080 TGTACA
BspDI 1457 ATCGAT AT^CGAT
4562 ATCGAT
BspHI 1463 TCATGA T^CATGA
3042 TCATGA
BspLU11I 3577 ACATGT A^CATGT
4721 ACATGT
BspMI 1173 GCAGGT ACCTGC(4/8)
2472 GCAGGT
BsrBI 280 GAGCGG CCGCTC(-3/-3)
403 GAGCGG
BsrGI 1218 TGTACA T^GTACA
4080 TGTACA
ClaI 1457 ATCGAT AT^CGAT
4562 ATCGAT
DraII 297 GGGACCC [GA]G^G[ACGT]CC[CT]
2070 AGGACCC
DsaI 157 CCGCGG C^C[GA][CT]GG
4684 CCATGG
EcoO109I 297 GGGACCC [GA]G^G[ACGT]CC[CT]
2070 AGGACCC
EcoRI 4600 GAATTC G^AATTC
5413 GAATTC
FauI 140 GCGGG CCCGC(4/6)
282 GCGGG
HpaI 2650 GTTAAC GTT^AAC
4660 GTTAAC
MaeII 2790 ACGT A^CGT
4355 ACGT
MluNI 2768 TGGCCA TGG^CCA
5714 TGGCCA
MscI 2768 TGGCCA TGG^CCA
5714 TGGCCA
NdeI 5023 CATATG CA^TATG
5300 CATATG
PflMI 4836 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4908 CCAGTTGCTGG
PpuMI 297 GGGACCC [GA]G^G[AT]CC[CT]
2070 AGGACCC
RcaI 1463 TCATGA T^CATGA
3042 TCATGA
ScaI 3694 AGTACT AGT^ACT
4109 AGTACT
SmlI 3172 CTTAAG C^T[CT][GA]AG
4093 CTCAAG
SspBI 1218 TGTACA T^GTACA
4080 TGTACA
SspI 4411 AATATT AAT^ATT
5155 AATATT
Van91I 4836 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4908 CCAGTTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BanII BbvI
BciVI BcnI BfaI BmyI BpmI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
EaeI EarI Eco81I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI