Restriction map for
pF1KA0562
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BalI BamHI
BanI BbeI BbrPI BciVI BfrI
BglII BlnI BsaHI BseAI BsiEI
BspEI BssHII BssSI Bst1107I BstZ17I
Bsu36I CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT22I FseI Hin1I HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI PacI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SwaI Van91I XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1075 GTAGAC GT^[AC][GT]AC
AgeI 2528 ACCGGT A^CCGGT
ApaI 2592 GGGCCC GGGCC^C
AspI 1338 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2618 TGCGCA TGC^GCA
BclI 1517 TGATCA T^GATCA
BglI 2594 GCCCAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2669 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2669 GCTCAGC GC^T[ACGT]AGC
BsaAI 289 TACGTG [CT]AC^GT[GA]
BsaBI 1099 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 455 GAGACC GGTCTC(1/5)
BseRI 1058 CTCCTC GAGGAG(10/8)
BsmBI 1879 GAGACG CGTCTC(1/5)
BsmI 1065 GCATTC GAATGC(1/-1)
Bsp1407I 1907 TGTACA T^GTACA
BspDI 1294 ATCGAT AT^CGAT
BspLU11I 1904 ACATGT A^CATGT
BspMI 2615 ACCTGC ACCTGC(4/8)
BsrGI 1907 TGTACA T^GTACA
BstEII 104 GGTCACC G^GT[ACGT]ACC
BstPI 104 GGTCACC G^GT[ACGT]ACC
CelII 2669 GCTCAGC GC^T[ACGT]AGC
ClaI 1294 ATCGAT AT^CGAT
DraIII 1570 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 779 CCGTGG C^C[GA][CT]GG
EaeI 2678 TGGCCG [CT]^GGCC[GA]
EcoO65I 104 GGTCACC G^GT[ACGT]ACC
EcoRV 2373 GATATC GAT^ATC
FauI 1625 CCCGC CCCGC(4/6)
FbaI 1517 TGATCA T^GATCA
FspI 2618 TGCGCA TGC^GCA
HaeII 743 AGCGCC [GA]GCGC^[CT]
HgaI 1634 GCGTC GACGC(5/10)
HincII 400 GTCAAC GT[CT]^[GA]AC
HindII 400 GTCAAC GT[CT]^[GA]AC
MamI 1099 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1293 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 2599 GCCGGC GCC^GGC
NgoMIV 2599 GCCGGC G^CCGGC
PaeR7I 494 CTCGAG C^TCGAG
PflFI 1338 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2528 ACCGGT A^CCGGT
PpuMI 536 AGGACCC [GA]G^G[AT]CC[CT]
SexAI 1709 ACCTGGT A^CC[AT]GGT
SspBI 1907 TGTACA T^GTACA
SspI 245 AATATT AAT^ATT
Tth111I 1338 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1292 CCATCGATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 494 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 1647 AAATTT [GA]^AATT[CT]
2132 AAATTC
AflIII 290 ACGTGT A^C[GA][CT]GT
1904 ACATGT
AlwNI 2027 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2207 CAGCCCCTG
ApoI 1647 AAATTT [GA]^AATT[CT]
2132 AAATTC
BbvCI 1060 CCTCAGC CCTCAGC(-5/-2)
1321 GCTGAGG
BcgI 534 CGAGGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2609 CGATGAACCTGC
BsaWI 2511 ACCGGA [AT]^CCGG[AT]
2528 ACCGGT
BsiWI 557 CGTACG C^GTACG
2762 CGTACG
BsmFI 1125 GTCCC GGGAC(10/14)
2534 GTCCC
BspHI 71 TCATGA T^CATGA
2544 TCATGA
BsrBI 268 GAGCGG CCGCTC(-3/-3)
1141 GAGCGG
BsrDI 717 CATTGC GCAATG(2/0)
1750 GCAATG
BsrFI 2528 ACCGGT [GA]^CCGG[CT]
2599 GCCGGC
BstBI 275 TTCGAA TT^CGAA
1889 TTCGAA
BstXI 6 CCACAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
513 CCACTACCTTGG
Cfr10I 2528 ACCGGT [GA]^CCGG[CT]
2599 GCCGGC
DraI 1419 TTTAAA TTT^AAA
1672 TTTAAA
HindIII 234 AAGCTT A^AGCTT
2099 AAGCTT
NspI 1235 GCATGT [GA]CATG^[CT]
1904 ACATGT
NspV 275 TTCGAA TT^CGAA
1889 TTCGAA
PleI 1123 GAGTC GAGTC(4/5)
1755 GAGTC
RcaI 71 TCATGA T^CATGA
2544 TCATGA
SacI 67 GAGCTC GAGCT^C
2748 GAGCTC
SapI 1457 GAAGAGC GCTCTTC(1/4)
2464 GAAGAGC
SfuI 275 TTCGAA TT^CGAA
1889 TTCGAA
SmaI 62 CCCGGG CCC^GGG
329 CCCGGG
SmlI 494 CTCGAG C^T[CT][GA]AG
1272 CTTGAG
StuI 1268 AGGCCT AGG^CCT
1325 AGGCCT
XbaI 546 TCTAGA T^CTAGA
2247 TCTAGA
XmaCI 62 CCCGGG C^CCGGG
329 CCCGGG
XmaI 62 CCCGGG C^CCGGG
329 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII