################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BalI BamHI BanI BbeI BbrPI BciVI BfrI BglII BlnI BsaHI BseAI BsiEI BspEI BssHII BssSI Bst1107I BstZ17I Bsu36I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FseI Hin1I HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI PacI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SwaI Van91I XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 1075 GTAGAC GT^[AC][GT]AC AgeI 2528 ACCGGT A^CCGGT ApaI 2592 GGGCCC GGGCC^C AspI 1338 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2618 TGCGCA TGC^GCA BclI 1517 TGATCA T^GATCA BglI 2594 GCCCAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2669 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2669 GCTCAGC GC^T[ACGT]AGC BsaAI 289 TACGTG [CT]AC^GT[GA] BsaBI 1099 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 455 GAGACC GGTCTC(1/5) BseRI 1058 CTCCTC GAGGAG(10/8) BsmBI 1879 GAGACG CGTCTC(1/5) BsmI 1065 GCATTC GAATGC(1/-1) Bsp1407I 1907 TGTACA T^GTACA BspDI 1294 ATCGAT AT^CGAT BspLU11I 1904 ACATGT A^CATGT BspMI 2615 ACCTGC ACCTGC(4/8) BsrGI 1907 TGTACA T^GTACA BstEII 104 GGTCACC G^GT[ACGT]ACC BstPI 104 GGTCACC G^GT[ACGT]ACC CelII 2669 GCTCAGC GC^T[ACGT]AGC ClaI 1294 ATCGAT AT^CGAT DraIII 1570 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 779 CCGTGG C^C[GA][CT]GG EaeI 2678 TGGCCG [CT]^GGCC[GA] EcoO65I 104 GGTCACC G^GT[ACGT]ACC EcoRV 2373 GATATC GAT^ATC FauI 1625 CCCGC CCCGC(4/6) FbaI 1517 TGATCA T^GATCA FspI 2618 TGCGCA TGC^GCA HaeII 743 AGCGCC [GA]GCGC^[CT] HgaI 1634 GCGTC GACGC(5/10) HincII 400 GTCAAC GT[CT]^[GA]AC HindII 400 GTCAAC GT[CT]^[GA]AC MamI 1099 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 1293 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 2599 GCCGGC GCC^GGC NgoMIV 2599 GCCGGC G^CCGGC PaeR7I 494 CTCGAG C^TCGAG PflFI 1338 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2528 ACCGGT A^CCGGT PpuMI 536 AGGACCC [GA]G^G[AT]CC[CT] SexAI 1709 ACCTGGT A^CC[AT]GGT SspBI 1907 TGTACA T^GTACA SspI 245 AATATT AAT^ATT Tth111I 1338 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1292 CCATCGATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 494 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 1647 AAATTT [GA]^AATT[CT] 2132 AAATTC AflIII 290 ACGTGT A^C[GA][CT]GT 1904 ACATGT AlwNI 2027 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 2207 CAGCCCCTG ApoI 1647 AAATTT [GA]^AATT[CT] 2132 AAATTC BbvCI 1060 CCTCAGC CCTCAGC(-5/-2) 1321 GCTGAGG BcgI 534 CGAGGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2609 CGATGAACCTGC BsaWI 2511 ACCGGA [AT]^CCGG[AT] 2528 ACCGGT BsiWI 557 CGTACG C^GTACG 2762 CGTACG BsmFI 1125 GTCCC GGGAC(10/14) 2534 GTCCC BspHI 71 TCATGA T^CATGA 2544 TCATGA BsrBI 268 GAGCGG CCGCTC(-3/-3) 1141 GAGCGG BsrDI 717 CATTGC GCAATG(2/0) 1750 GCAATG BsrFI 2528 ACCGGT [GA]^CCGG[CT] 2599 GCCGGC BstBI 275 TTCGAA TT^CGAA 1889 TTCGAA BstXI 6 CCACAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 513 CCACTACCTTGG Cfr10I 2528 ACCGGT [GA]^CCGG[CT] 2599 GCCGGC DraI 1419 TTTAAA TTT^AAA 1672 TTTAAA HindIII 234 AAGCTT A^AGCTT 2099 AAGCTT NspI 1235 GCATGT [GA]CATG^[CT] 1904 ACATGT NspV 275 TTCGAA TT^CGAA 1889 TTCGAA PleI 1123 GAGTC GAGTC(4/5) 1755 GAGTC RcaI 71 TCATGA T^CATGA 2544 TCATGA SacI 67 GAGCTC GAGCT^C 2748 GAGCTC SapI 1457 GAAGAGC GCTCTTC(1/4) 2464 GAAGAGC SfuI 275 TTCGAA TT^CGAA 1889 TTCGAA SmaI 62 CCCGGG CCC^GGG 329 CCCGGG SmlI 494 CTCGAG C^T[CT][GA]AG 1272 CTTGAG StuI 1268 AGGCCT AGG^CCT 1325 AGGCCT XbaI 546 TCTAGA T^CTAGA 2247 TCTAGA XmaCI 62 CCCGGG C^CCGGG 329 CCCGGG XmaI 62 CCCGGG C^CCGGG 329 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII