Restriction map for
pF1KA0567
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BcnI BlnI BlpI
Bpu1102I BsaHI BsiEI BsiWI BsmFI
Bsp1407I BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII
BstPI CelII Cfr10I CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoT14I EcoT22I FseI
HaeII HgaI Hin1I HpaI KasI
KpnI KspI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfoI
SgrAI SmaI Sse8387I SspBI StuI
StyI SwaI Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 2794 CGCG CG^CG
AflII 778 CTTAAG C^TTAAG
AocI 551 CCTCAGG CC^T[ACGT]AGG
AseI 1207 ATTAAT AT^TAAT
AsnI 1207 ATTAAT AT^TAAT
AspHI 2410 GAGCAC G[AT]GC[AT]^C
AviII 1920 TGCGCA TGC^GCA
BalI 265 TGGCCA TGG^CCA
BclI 357 TGATCA T^GATCA
BfrI 778 CTTAAG C^TTAAG
BglI 19 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1062 AGATCT A^GATCT
BmyI 2410 GAGCAC G[AGT]GC[ACT]^C
BpmI 974 CTCCAG CTGGAG(16/14)
BsaAI 209 TACGTA [CT]AC^GT[GA]
BsaBI 2198 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 667 CTGCAC GTGCAG(16/14)
BsiHKAI 2410 GAGCAC G[AT]GC[AT]^C
BsmBI 2263 CGTCTC CGTCTC(1/5)
Bsp1286I 2410 GAGCAC G[AGT]GC[ACT]^C
BspDI 439 ATCGAT AT^CGAT
BspLU11I 797 ACATGT A^CATGT
Bst1107I 2645 GTATAC GTA^TAC
BstBI 1094 TTCGAA TT^CGAA
BstUI 2794 CGCG CG^CG
BstXI 1837 CCAAAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2645 GTATAC GTA^TAC
Bsu36I 551 CCTCAGG CC^T[ACGT]AGG
ClaI 439 ATCGAT AT^CGAT
DsaI 865 CCACGG C^C[GA][CT]GG
EaeI 265 TGGCCA [CT]^GGCC[GA]
Eco81I 551 CCTCAGG CC^T[ACGT]AGG
EcoRV 1960 GATATC GAT^ATC
FbaI 357 TGATCA T^GATCA
FspI 1920 TGCGCA TGC^GCA
HincII 1189 GTTGAC GT[CT]^[GA]AC
HindII 1189 GTTGAC GT[CT]^[GA]AC
MamI 2198 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2285 CAATTG C^AATTG
MluI 2793 ACGCGT A^CGCGT
MluNI 265 TGGCCA TGG^CCA
MscI 265 TGGCCA TGG^CCA
MslI 1950 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2285 CAATTG C^AATTG
MvnI 2794 CGCG CG^CG
NspV 1094 TTCGAA TT^CGAA
PpuMI 999 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1207 ATTAAT AT^TAAT
PstI 2254 CTGCAG CTGCA^G
PvuII 1916 CAGCTG CAG^CTG
SexAI 1197 ACCAGGT A^CC[AT]GGT
SfiI 18 GGCCGCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1094 TTCGAA TT^CGAA
SnaBI 209 TACGTA TAC^GTA
SpeI 904 ACTAGT A^CTAGT
SspI 936 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1405 GTAGAC GT^[AC][GT]AC
2645 GTATAC
AccIII 561 TCCGGA T^CCGGA
2450 TCCGGA
AfaI 1124 GTAC GT^AC
1666 GTAC
AspI 8 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1348 GACTTGGTC
AvaI 1034 CTCGGG C^[CT]CG[GA]G
2468 CCCGAG
BciVI 2271 GGATAC GTATCC(6/5)
2502 GGATAC
BfaI 313 CTAG C^TAG
905 CTAG
BsaI 1138 GAGACC GGTCTC(1/5)
2023 GAGACC
BseAI 561 TCCGGA T^CCGGA
2450 TCCGGA
BseRI 2407 GAGGAG GAGGAG(10/8)
2471 GAGGAG
BsmI 2617 GAATGC GAATGC(1/-1)
761 GCATTC
BsoBI 1034 CTCGGG C^[CT]CG[GA]G
2468 CCCGAG
BspEI 561 TCCGGA T^CCGGA
2450 TCCGGA
BsrDI 2217 GCAATG GCAATG(2/0)
2621 GCAATG
CfoI 1921 GCGC GCG^C
2652 GCGC
DraI 1021 TTTAAA TTT^AAA
2535 TTTAAA
DraII 999 AGGTCCT [GA]G^G[ACGT]CC[CT]
1161 AGGCCCT
DraIII 1006 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1609 CACCAAGTG
EcoO109I 999 AGGTCCT [GA]G^G[ACGT]CC[CT]
1161 AGGCCCT
EcoRI 1572 GAATTC G^AATTC
1932 GAATTC
FauI 2045 CCCGC CCCGC(4/6)
1755 GCGGG
HhaI 1921 GCGC GCG^C
2652 GCGC
HinP1I 1921 GCGC G^CGC
2652 GCGC
MaeI 313 CTAG C^TAG
905 CTAG
MroI 561 TCCGGA T^CCGGA
2450 TCCGGA
MspA1I 20 CCGCTG C[AC]G^C[GT]G
1916 CAGCTG
PflFI 8 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1348 GACTTGGTC
RsaI 1124 GTAC GT^AC
1666 GTAC
SapI 1467 GCTCTTC GCTCTTC(1/4)
2863 GCTCTTC
SfcI 1171 CTACAG C^T[GA][CT]AG
2254 CTGCAG
SphI 1595 GCATGC GCATG^C
2654 GCATGC
Tth111I 8 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1348 GACTTGGTC
XcmI 1400 CCAAAGTAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2372 CCAAGAATGAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AflIII AluI AlwI
ApoI Asp700I AvaII BbsI BbvI
BpuAI BsaJI BsaWI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI