Restriction map for
pF1KA0602
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AgeI ApaI ApoI AseI
AsnI AviII BamHI BcgI BclI
BfrI BseAI BsiWI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstZ17I CpoI
DraI EcoRI EcoRV EcoT22I FbaI
FspI HpaI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 1799 GGTACC G^GTACC
AocI 1159 CCTGAGG CC^T[ACGT]AGG
AscI 31 GGCGCGCC GG^CGCGCC
Asp718I 1799 GGTACC G^GTACC
AvrII 1803 CCTAGG C^CTAGG
BbeI 2266 GGCGCC GGCGC^C
BfaI 1804 CTAG C^TAG
BglII 503 AGATCT A^GATCT
BlnI 1803 CCTAGG C^CTAGG
BlpI 456 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 456 GCTGAGC GC^T[ACGT]AGC
BsaBI 504 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1856 GAGACC GGTCTC(1/5)
BsaWI 2384 TCCGGT [AT]^CCGG[AT]
BsiEI 497 CGGCCG CG[GA][CT]^CG
BsmBI 1605 CGTCTC CGTCTC(1/5)
BspDI 711 ATCGAT AT^CGAT
BstEII 2457 GGTCACC G^GT[ACGT]ACC
BstPI 2457 GGTCACC G^GT[ACGT]ACC
Bsu36I 1159 CCTGAGG CC^T[ACGT]AGG
CelII 456 GCTGAGC GC^T[ACGT]AGC
ClaI 711 ATCGAT AT^CGAT
DraIII 1786 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 497 CGGCCG C^GGCCG
EclXI 497 CGGCCG C^GGCCG
Eco52I 497 CGGCCG C^GGCCG
Eco81I 1159 CCTGAGG CC^T[ACGT]AGG
EcoNI 1259 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2457 GGTCACC G^GT[ACGT]ACC
FseI 561 GGCCGGCC GGCCGG^CC
HincII 1492 GTCAAC GT[CT]^[GA]AC
HindII 1492 GTCAAC GT[CT]^[GA]AC
HindIII 328 AAGCTT A^AGCTT
KasI 2266 GGCGCC G^GCGCC
KpnI 1799 GGTACC GGTAC^C
KspI 398 CCGCGG CCGC^GG
MaeI 1804 CTAG C^TAG
MamI 504 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2266 GGCGCC GG^CGCC
PaeR7I 984 CTCGAG C^TCGAG
PshAI 2451 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 987 GAGCTC GAGCT^C
SacII 398 CCGCGG CCGC^GG
SexAI 1264 ACCAGGT A^CC[AT]GGT
SfoI 2266 GGCGCC GGC^GCC
TfiI 218 GAATC G^A[AT]TC
XhoI 984 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
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Alw44I 1560 GTGCAC G^TGCAC
2376 GTGCAC
AlwNI 451 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1840 CAGGACCTG
ApaLI 1560 GTGCAC G^TGCAC
2376 GTGCAC
Asp700I 66 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2667 GAAGCACTTC
BalI 447 TGGCCA TGG^CCA
1793 TGGCCA
BanI 1799 GGTACC G^G[CT][GA]CC
2266 GGCGCC
BbrPI 1563 CACGTG CAC^GTG
2662 CACGTG
BciVI 1613 GGATAC GTATCC(6/5)
297 GTATCC
BsaAI 1563 CACGTG [CT]AC^GT[GA]
2662 CACGTG
BspMI 2348 ACCTGC ACCTGC(4/8)
2286 GCAGGT
BsrFI 562 GCCGGC [GA]^CCGG[CT]
2552 GCCGGC
BssHII 32 GCGCGC G^CGCGC
41 GCGCGC
BstYI 503 AGATCT [GA]^GATC[CT]
1412 AGATCC
Cfr10I 562 GCCGGC [GA]^CCGG[CT]
2552 GCCGGC
DrdI 922 GACGACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2347 GACCTGCCTGTC
MflI 503 AGATCT [GA]^GATC[CT]
1412 AGATCC
MluNI 447 TGGCCA TGG^CCA
1793 TGGCCA
MscI 447 TGGCCA TGG^CCA
1793 TGGCCA
NaeI 562 GCCGGC GCC^GGC
2552 GCCGGC
NgoMIV 562 GCCGGC G^CCGGC
2552 GCCGGC
NspI 1754 ACATGC [GA]CATG^[CT]
2585 ACATGC
PflMI 1785 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1839 CCAGGACCTGG
PmaCI 1563 CACGTG CAC^GTG
2662 CACGTG
PmlI 1563 CACGTG CAC^GTG
2662 CACGTG
SmlI 984 CTCGAG C^T[CT][GA]AG
2371 CTCAAG
Tsp509I 1636 AATT ^AATT
2091 AATT
Van91I 1785 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1839 CCAGGACCTGG
XcmI 77 CCACCTGGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
251 CCAGTGGACAAGTGG
XhoII 503 AGATCT [GA]^GATC[CT]
1412 AGATCC
XmnI 66 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2667 GAAGCACTTC
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# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AhdI AluI AlwI Aor51HI AspEI
AspHI AspI AvaI AvaII BanII
BbsI BbvCI BbvI BcnI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI Eco47III EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflFI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI StuI StyI
TaqI TseI Tsp45I TspRI Tth111I
TthHB8I