Restriction map for
pF1KA0625
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# NO CUT ENZYMES ( 76 enzymes) #
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Acc65I AccII AccIII AclI AgeI
AhdI AocI AscI Asp718I AspEI
AviII AvrII BbeI BbvCI BglI
BlnI BlpI Bpu1102I BseAI BsiEI
BsiWI BspEI BspMI BsrBI BsrFI
BssHII BstEII BstPI BstUI Bsu36I
CelII Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
FseI FspI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NgoMIV NheI NotI NruI PacI
PflMI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SwaI Van91I XmaCI
XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 1887 GACGTC GACGT^C
Alw44I 6017 GTGCAC G^TGCAC
Aor51HI 56 AGCGCT AGC^GCT
ApaI 5158 GGGCCC GGGCC^C
ApaLI 6017 GTGCAC G^TGCAC
BanI 7333 GGTGCC G^G[CT][GA]CC
BbrPI 7848 CACGTG CAC^GTG
BciVI 4889 GGATAC GTATCC(6/5)
BspDI 804 ATCGAT AT^CGAT
Bst1107I 1066 GTATAC GTA^TAC
BstAPI 1569 GCATTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 7016 TTCGAA TT^CGAA
BstZ17I 1066 GTATAC GTA^TAC
CfoI 57 GCGC GCG^C
ClaI 804 ATCGAT AT^CGAT
DraIII 4638 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 56 AGCGCT AGC^GCT
EcoRV 1551 GATATC GAT^ATC
HaeII 56 AGCGCT [GA]GCGC^[CT]
HgaI 6404 GACGC GACGC(5/10)
HhaI 57 GCGC GCG^C
HinP1I 57 GCGC G^CGC
HpaI 385 GTTAAC GTT^AAC
NcoI 755 CCATGG C^CATGG
NspV 7016 TTCGAA TT^CGAA
PaeR7I 7680 CTCGAG C^TCGAG
PmaCI 7848 CACGTG CAC^GTG
PmlI 7848 CACGTG CAC^GTG
PshAI 2833 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 7016 TTCGAA TT^CGAA
SphI 4247 GCATGC GCATG^C
StuI 3523 AGGCCT AGG^CCT
XhoI 7680 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcyI 1887 GACGTC G[GA]^CG[CT]C
6404 GACGCC
AflII 1261 CTTAAG C^TTAAG
5790 CTTAAG
AseI 3981 ATTAAT AT^TAAT
6154 ATTAAT
AsnI 3981 ATTAAT AT^TAAT
6154 ATTAAT
AspI 1354 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6134 GACTGGGTC
AvaI 7680 CTCGAG C^[CT]CG[GA]G
7837 CCCGAG
BalI 859 TGGCCA TGG^CCA
6874 TGGCCA
BcgI 6292 CGAGCTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1055 GCAGCCAGATCG
BfrI 1261 CTTAAG C^TTAAG
5790 CTTAAG
BsaBI 451 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6860 GATGTTCATC
BsaHI 1887 GACGTC G[GA]^CG[CT]C
6404 GACGCC
BsaWI 79 TCCGGT [AT]^CCGG[AT]
1023 ACCGGA
BsgI 6456 CTGCAC GTGCAG(16/14)
1606 GTGCAG
BsmBI 202 CGTCTC CGTCTC(1/5)
4865 CGTCTC
BsoBI 7680 CTCGAG C^[CT]CG[GA]G
7837 CCCGAG
BspHI 6047 TCATGA T^CATGA
7596 TCATGA
BssSI 7238 CACGAG CACGAG(-5/-1)
6010 CTCGTG
BstXI 488 CCAATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7689 CCAGCATCCTGG
FauI 7768 CCCGC CCCGC(4/6)
7812 GCGGG
Hin1I 1887 GACGTC G[GA]^CG[CT]C
6404 GACGCC
MamI 451 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6860 GATGTTCATC
MluNI 859 TGGCCA TGG^CCA
6874 TGGCCA
MscI 859 TGGCCA TGG^CCA
6874 TGGCCA
PflFI 1354 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6134 GACTGGGTC
PshBI 3981 ATTAAT AT^TAAT
6154 ATTAAT
PstI 6699 CTGCAG CTGCA^G
7405 CTGCAG
RcaI 6047 TCATGA T^CATGA
7596 TCATGA
SacI 6293 GAGCTC GAGCT^C
7882 GAGCTC
ScaI 2404 AGTACT AGT^ACT
4807 AGTACT
SpeI 1729 ACTAGT A^CTAGT
4030 ACTAGT
SspI 5039 AATATT AAT^ATT
7656 AATATT
Tth111I 1354 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6134 GACTGGGTC
XbaI 1891 TCTAGA T^CTAGA
6258 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BamHI BanII BbsI
BbvI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspLU11I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI