Restriction map for
pF1KA0683
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
AspHI AviII AvrII BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BsaBI BsaWI BseAI BsiHKAI BsiWI
BsmI Bsp1407I BspEI BspHI BsrDI
BsrGI BssSI Bst1107I BstBI BstZ17I
DraI DrdI EcoRV EcoT22I FbaI
FspI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacI
SacII SalI SbfI ScaI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 702 GGTACC G^GTACC
AccI 1624 GTCTAC GT^[AC][GT]AC
AflIII 1496 ACATGT A^C[GA][CT]GT
AhdI 26 GACTCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 702 GGTACC G^GTACC
AspEI 26 GACTCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1566 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1247 GGATCC G^GATCC
BfaI 1871 CTAG C^TAG
BsaAI 2293 TACGTG [CT]AC^GT[GA]
BsiEI 2315 CGGCCG CG[GA][CT]^CG
BspDI 2284 ATCGAT AT^CGAT
BspLU11I 1496 ACATGT A^CATGT
BssHII 2350 GCGCGC G^CGCGC
BstAPI 945 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2133 GGTGACC G^GT[ACGT]ACC
BstPI 2133 GGTGACC G^GT[ACGT]ACC
BstXI 1154 CCAGCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2284 ATCGAT AT^CGAT
CpoI 779 CGGACCG CG^G[AT]CCG
DraIII 1923 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2315 CGGCCG C^GGCCG
Eam1105I 26 GACTCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2315 CGGCCG C^GGCCG
Eco52I 2315 CGGCCG C^GGCCG
EcoO65I 2133 GGTGACC G^GT[ACGT]ACC
EcoRI 2257 GAATTC G^AATTC
FseI 2085 GGCCGGCC GGCCGG^CC
HincII 2215 GTTAAC GT[CT]^[GA]AC
HindII 2215 GTTAAC GT[CT]^[GA]AC
HindIII 904 AAGCTT A^AGCTT
HpaI 2215 GTTAAC GTT^AAC
KpnI 702 GGTACC GGTAC^C
MaeI 1871 CTAG C^TAG
MseI 2216 TTAA T^TAA
NspI 1496 ACATGT [GA]CATG^[CT]
PaeR7I 1303 CTCGAG C^TCGAG
PflFI 1566 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 779 CGGACCG CG^G[AT]CCG
SapI 1518 GAAGAGC GCTCTTC(1/4)
SexAI 860 ACCTGGT A^CC[AT]GGT
SfiI 1764 GGCCCCGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I 2216 TTAA T^TAA
Tth111I 1566 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1303 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1273 AAATTC [GA]^AATT[CT]
2257 GAATTC
AfaI 703 GTAC GT^AC
1281 GTAC
AocI 832 CCTGAGG CC^T[ACGT]AGG
2189 CCTTAGG
ApoI 1273 AAATTC [GA]^AATT[CT]
2257 GAATTC
Asp700I 40 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
546 GAACTACTTC
BlpI 207 GCTGAGC GC^T[ACGT]AGC
2334 GCTCAGC
Bpu1102I 207 GCTGAGC GC^T[ACGT]AGC
2334 GCTCAGC
BsaI 313 GAGACC GGTCTC(1/5)
1399 GAGACC
BsgI 87 CTGCAC GTGCAG(16/14)
739 CTGCAC
BspMI 737 ACCTGC ACCTGC(4/8)
999 GCAGGT
BsrBI 988 CCGCTC CCGCTC(-3/-3)
1999 GAGCGG
BsrFI 392 GCCGGC [GA]^CCGG[CT]
2086 GCCGGC
BstYI 686 AGATCC [GA]^GATC[CT]
1247 GGATCC
Bsu36I 832 CCTGAGG CC^T[ACGT]AGG
2189 CCTTAGG
CelII 207 GCTGAGC GC^T[ACGT]AGC
2334 GCTCAGC
Cfr10I 392 GCCGGC [GA]^CCGG[CT]
2086 GCCGGC
EarI 1285 GAAGAG CTCTTC(1/4)
1518 GAAGAG
Eco81I 832 CCTGAGG CC^T[ACGT]AGG
2189 CCTTAGG
Ksp632I 1285 GAAGAG CTCTTC(1/4)
1518 GAAGAG
MflI 686 AGATCC [GA]^GATC[CT]
1247 GGATCC
NaeI 392 GCCGGC GCC^GGC
2086 GCCGGC
NgoMIV 392 GCCGGC G^CCGGC
2086 GCCGGC
PvuII 1441 CAGCTG CAG^CTG
2161 CAGCTG
RsaI 703 GTAC GT^AC
1281 GTAC
SfaNI 2287 GATGC GCATC(5/9)
1683 GCATC
StuI 1873 AGGCCT AGG^CCT
2125 AGGCCT
Tsp509I 1274 AATT ^AATT
2258 AATT
XcmI 263 CCAGCTTCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2222 CCACGGTGGCTGTGG
XhoII 686 AGATCC [GA]^GATC[CT]
1247 GGATCC
XmnI 40 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
546 GAACTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI Aor51HI ApaI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcgI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI Sau3AI Sau96I ScrFI
SfcI SfoI SmlI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I