Restriction map for
pF1KA0687
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BbeI BbrPI
BbvCI BciVI BclI BglI BglII
BlnI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BspDI BspEI
BspMI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRI FbaI FseI
FspI Hin1I HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NarI NcoI NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RsrII SacI SacII
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 1217 CGCG CG^CG
AclI 1094 AACGTT AA^CGTT
AflII 904 CTTAAG C^TTAAG
AlwNI 1513 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 1887 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfrI 904 CTTAAG C^TTAAG
BlpI 526 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 526 GCTCAGC GC^T[ACGT]AGC
BsaAI 2336 TACGTG [CT]AC^GT[GA]
BsaBI 1481 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 489 GAATGC GAATGC(1/-1)
Bsp1407I 2439 TGTACA T^GTACA
BspHI 3077 TCATGA T^CATGA
BsrFI 1842 GCCGGC [GA]^CCGG[CT]
BsrGI 2439 TGTACA T^GTACA
BstAPI 679 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1217 CGCG CG^CG
BstXI 668 CCATTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 526 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1842 GCCGGC [GA]^CCGG[CT]
DraIII 2102 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3083 CCTTAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 457 GATATC GAT^ATC
EcoT22I 706 ATGCAT ATGCA^T
FauI 54 GCGGG CCCGC(4/6)
HaeII 1514 AGCGCC [GA]GCGC^[CT]
HgaI 1765 GCGTC GACGC(5/10)
HindIII 1167 AAGCTT A^AGCTT
MamI 1481 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1217 CGCG CG^CG
NaeI 1842 GCCGGC GCC^GGC
NdeI 2831 CATATG CA^TATG
NgoMIV 1842 GCCGGC G^CCGGC
NsiI 706 ATGCAT ATGCA^T
PflFI 1887 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1094 AACGTT AA^CGTT
PvuII 529 CAGCTG CAG^CTG
RcaI 3077 TCATGA T^CATGA
SalI 125 GTCGAC G^TCGAC
SapI 1105 GCTCTTC GCTCTTC(1/4)
SmaI 1429 CCCGGG CCC^GGG
SspBI 2439 TGTACA T^GTACA
Tth111I 1887 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1429 CCCGGG C^CCGGG
XmaI 1429 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AvaI 1180 CCCGAG C^[CT]CG[GA]G
1429 CCCGGG
BalI 1604 TGGCCA TGG^CCA
2229 TGGCCA
BbsI 379 GAAGAC GAAGAC(2/6)
2261 GAAGAC
BcgI 9 CGACTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1868 GCACTCTAATCG
BpuAI 379 GAAGAC GAAGAC(2/6)
2261 GAAGAC
BsoBI 1180 CCCGAG C^[CT]CG[GA]G
1429 CCCGGG
BspLU11I 129 ACATGT A^CATGT
2703 ACATGT
CfoI 1216 GCGC GCG^C
1515 GCGC
HhaI 1216 GCGC GCG^C
1515 GCGC
HinP1I 1216 GCGC G^CGC
1515 GCGC
HincII 125 GTCGAC GT[CT]^[GA]AC
508 GTTGAC
HindII 125 GTCGAC GT[CT]^[GA]AC
508 GTTGAC
MluNI 1604 TGGCCA TGG^CCA
2229 TGGCCA
MscI 1604 TGGCCA TGG^CCA
2229 TGGCCA
MspA1I 529 CAGCTG C[AC]G^C[GT]G
826 CAGCGG
PstI 1075 CTGCAG CTGCA^G
1279 CTGCAG
XcmI 155 CCATCAAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2903 CCAATCAGACAATGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
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AccI AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI AspHI
AvaII BamHI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII EaeI
EarI Eco81I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII