Restriction map for
pF1KA0732
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# NO CUT ENZYMES ( 69 enzymes) #
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AatII AclI AflIII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
AspEI BsaBI BsiEI BsiWI BsmBI
BspDI BspLU11I BsrFI BssHII BssSI
BstBI BstEII BstPI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FseI HpaI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SbfI ScaI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 1815 TCCGGA T^CCGGA
AflII 2968 CTTAAG C^TTAAG
AviII 2369 TGCGCA TGC^GCA
AvrII 364 CCTAGG C^CTAGG
BbeI 2297 GGCGCC GGCGC^C
BbrPI 4239 CACGTG CAC^GTG
BbvCI 802 GCTGAGG CCTCAGC(-5/-2)
BciVI 1557 GTATCC GTATCC(6/5)
BfrI 2968 CTTAAG C^TTAAG
BlnI 364 CCTAGG C^CTAGG
BsaI 2434 GAGACC GGTCTC(1/5)
BseAI 1815 TCCGGA T^CCGGA
Bsp1407I 3233 TGTACA T^GTACA
BspEI 1815 TCCGGA T^CCGGA
BspHI 2417 TCATGA T^CATGA
BsrGI 3233 TGTACA T^GTACA
Bst1107I 2331 GTATAC GTA^TAC
BstAPI 2183 GCATGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2331 GTATAC GTA^TAC
EcoRI 217 GAATTC G^AATTC
EcoT22I 2181 ATGCAT ATGCA^T
FspI 2369 TGCGCA TGC^GCA
HindIII 2344 AAGCTT A^AGCTT
KasI 2297 GGCGCC G^GCGCC
KspI 702 CCGCGG CCGC^GG
MroI 1815 TCCGGA T^CCGGA
NarI 2297 GGCGCC GG^CGCC
NsiI 2181 ATGCAT ATGCA^T
PaeR7I 3940 CTCGAG C^TCGAG
PflMI 610 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4239 CACGTG CAC^GTG
PmlI 4239 CACGTG CAC^GTG
PshAI 3694 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2417 TCATGA T^CATGA
SacII 702 CCGCGG CCGC^GG
SalI 3174 GTCGAC G^TCGAC
SapI 3424 GAAGAGC GCTCTTC(1/4)
SexAI 509 ACCAGGT A^CC[AT]GGT
SfiI 66 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2297 GGCGCC GGC^GCC
SphI 2813 GCATGC GCATG^C
SspBI 3233 TGTACA T^GTACA
Van91I 610 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3940 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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Acc65I 2210 GGTACC G^GTACC
2261 GGTACC
AcsI 217 GAATTC [GA]^AATT[CT]
2479 GAATTT
AocI 3001 CCTGAGG CC^T[ACGT]AGG
3319 CCTCAGG
ApaI 1596 GGGCCC GGGCC^C
4244 GGGCCC
ApoI 217 GAATTC [GA]^AATT[CT]
2479 GAATTT
Asp718I 2210 GGTACC G^GTACC
2261 GGTACC
AspHI 2026 GAGCTC G[AT]GC[AT]^C
3718 GAGCTC
AspI 1644 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2350 GACGCTGTC
BamHI 50 GGATCC G^GATCC
1980 GGATCC
BfaI 365 CTAG C^TAG
2207 CTAG
BsaAI 3334 TACGTG [CT]AC^GT[GA]
4239 CACGTG
BsaWI 1815 TCCGGA [AT]^CCGG[AT]
4175 ACCGGA
BsgI 1741 CTGCAC GTGCAG(16/14)
2955 CTGCAC
BsiHKAI 2026 GAGCTC G[AT]GC[AT]^C
3718 GAGCTC
BsrBI 735 CCGCTC CCGCTC(-3/-3)
2889 CCGCTC
BsrDI 2280 CATTGC GCAATG(2/0)
3995 GCAATG
Bsu36I 3001 CCTGAGG CC^T[ACGT]AGG
3319 CCTCAGG
Eco81I 3001 CCTGAGG CC^T[ACGT]AGG
3319 CCTCAGG
HincII 3174 GTCGAC GT[CT]^[GA]AC
4164 GTTGAC
HindII 3174 GTCGAC GT[CT]^[GA]AC
4164 GTTGAC
KpnI 2210 GGTACC GGTAC^C
2261 GGTACC
MaeI 365 CTAG C^TAG
2207 CTAG
PflFI 1644 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2350 GACGCTGTC
SacI 2026 GAGCTC GAGCT^C
3718 GAGCTC
Tth111I 1644 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2350 GACGCTGTC
XcmI 309 CCAAGAGCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1106 CCAGGGATGATATGG
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# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI Aor51HI Asp700I
AvaI AvaII BalI BanI BanII
BbsI BbvI BcgI BclI BcnI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI