Restriction map for
pF1KA0740
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccIII AflII AflIII AhdI
Alw44I ApaLI AscI Asp700I AspEI
AspI AviII BamHI BbvCI BcnI
BfrI BglI BglII BlpI Bpu1102I
BsaBI BsaI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraIII EagI
Eam1105I EclXI Eco52I EcoO65I EcoT22I
FseI FspI HindIII HpaI KspI
MamI MfeI MluI MroI MunI
NaeI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PleI PmeI PvuI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
Tth111I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 1964 GGTACC G^GTACC
AccI 979 GTCGAC GT^[AC][GT]AC
AcyI 467 GGCGCC G[GA]^CG[CT]C
AgeI 720 ACCGGT A^CCGGT
AlwI 1656 GGATC GGATC(4/5)
AocI 1021 CCTCAGG CC^T[ACGT]AGG
Aor51HI 618 AGCGCT AGC^GCT
AseI 1674 ATTAAT AT^TAAT
AsnI 1674 ATTAAT AT^TAAT
Asp718I 1964 GGTACC G^GTACC
AspHI 1789 GTGCTC G[AT]GC[AT]^C
AvrII 1012 CCTAGG C^CTAGG
BalI 137 TGGCCA TGG^CCA
BbeI 467 GGCGCC GGCGC^C
BbrPI 145 CACGTG CAC^GTG
BcgI 2050 CGAAAGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 261 TGATCA T^GATCA
BfaI 1013 CTAG C^TAG
BlnI 1012 CCTAGG C^CTAGG
BsaHI 467 GGCGCC G[GA]^CG[CT]C
BseRI 1000 GAGGAG GAGGAG(10/8)
BsiHKAI 1789 GTGCTC G[AT]GC[AT]^C
BsrBI 1939 GAGCGG CCGCTC(-3/-3)
BsrFI 720 ACCGGT [GA]^CCGG[CT]
BssSI 1761 CACGAG CACGAG(-5/-1)
BstYI 1656 GGATCT [GA]^GATC[CT]
Bsu36I 1021 CCTCAGG CC^T[ACGT]AGG
Cfr10I 720 ACCGGT [GA]^CCGG[CT]
DraI 1465 TTTAAA TTT^AAA
DrdI 1207 GACGCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 861 CTCTTC CTCTTC(1/4)
Eco47III 618 AGCGCT AGC^GCT
Eco81I 1021 CCTCAGG CC^T[ACGT]AGG
EcoNI 1056 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2067 GAATTC G^AATTC
EcoRV 1599 GATATC GAT^ATC
FbaI 261 TGATCA T^GATCA
Hin1I 467 GGCGCC G[GA]^CG[CT]C
KasI 467 GGCGCC G^GCGCC
KpnI 1964 GGTACC GGTAC^C
Ksp632I 861 CTCTTC CTCTTC(1/4)
MaeI 1013 CTAG C^TAG
MflI 1656 GGATCT [GA]^GATC[CT]
MluNI 137 TGGCCA TGG^CCA
MscI 137 TGGCCA TGG^CCA
NarI 467 GGCGCC GG^CGCC
NcoI 749 CCATGG C^CATGG
NdeI 287 CATATG CA^TATG
PaeR7I 1318 CTCGAG C^TCGAG
PflMI 1827 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 720 ACCGGT A^CCGGT
PmaCI 145 CACGTG CAC^GTG
PmlI 145 CACGTG CAC^GTG
PpuMI 1313 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1191 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1674 ATTAAT AT^TAAT
PstI 1916 CTGCAG CTGCA^G
PvuII 130 CAGCTG CAG^CTG
SalI 979 GTCGAC G^TCGAC
SfaNI 2037 GCATC GCATC(5/9)
SfoI 467 GGCGCC GGC^GCC
SwaI 1464 ATTTAAAT ATTT^AAAT
Van91I 1827 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1760 CCACGAGTGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1318 CTCGAG C^TCGAG
XhoII 1656 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 83 CGCG CG^CG
178 CGCG
AclI 31 AACGTT AA^CGTT
1446 AACGTT
AfaI 174 GTAC GT^AC
1965 GTAC
AlwNI 130 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1091 CAGTTCCTG
ApaI 1005 GGGCCC GGGCC^C
1183 GGGCCC
AvaI 206 CTCGGG C^[CT]CG[GA]G
1318 CTCGAG
BanII 1005 GGGCCC G[GA]GC[CT]^C
1183 GGGCCC
BbsI 78 GAAGAC GAAGAC(2/6)
1041 GTCTTC
BciVI 249 GGATAC GTATCC(6/5)
366 GTATCC
BpmI 1243 CTCCAG CTGGAG(16/14)
2075 CTCCAG
BpuAI 78 GAAGAC GAAGAC(2/6)
1041 GTCTTC
BsaAI 145 CACGTG [CT]AC^GT[GA]
1402 CACGTA
BsaWI 720 ACCGGT [AT]^CCGG[AT]
1114 ACCGGA
BsgI 1860 CTGCAC GTGCAG(16/14)
1088 GTGCAG
BsmFI 754 GGGAC GGGAC(10/14)
1260 GGGAC
BsoBI 206 CTCGGG C^[CT]CG[GA]G
1318 CTCGAG
BspHI 1355 TCATGA T^CATGA
1373 TCATGA
BspMI 638 ACCTGC ACCTGC(4/8)
293 GCAGGT
BstUI 83 CGCG CG^CG
178 CGCG
BstXI 151 CCAACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1187 CCATGACTGTGG
DsaI 71 CCGTGG C^C[GA][CT]GG
749 CCATGG
EaeI 137 TGGCCA [CT]^GGCC[GA]
1835 TGGCCG
HaeII 467 GGCGCC [GA]GCGC^[CT]
618 AGCGCT
HapII 721 CCGG C^CGG
1115 CCGG
HinfI 904 GAATC G^A[ACGT]TC
1017 GATTC
HpaII 721 CCGG C^CGG
1115 CCGG
HphI 61 GGTGA GGTGA(8/7)
259 GGTGA
MaeIII 62 GTGAC ^GT[ACGT]AC
1459 GTGAC
MspI 721 CCGG C^CGG
1115 CCGG
MvnI 83 CGCG CG^CG
178 CGCG
Psp1406I 31 AACGTT AA^CGTT
1446 AACGTT
RcaI 1355 TCATGA T^CATGA
1373 TCATGA
RsaI 174 GTAC GT^AC
1965 GTAC
SfcI 1512 CTGTAG C^T[GA][CT]AG
1916 CTGCAG
SmlI 1318 CTCGAG C^T[CT][GA]AG
2043 CTCAAG
SphI 1139 GCATGC GCATG^C
1731 GCATGC
TfiI 904 GAATC G^A[AT]TC
1017 GATTC
Tsp45I 62 GTGAC ^GT[GC]AC
1459 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BanI BbvI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HincII HindII
ItaI MaeII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI Sau3AI
Sau96I ScrFI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I