Restriction map for
pF1KA0743
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccIII AclI AcsI AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AviII AvrII BamHI BbeI BbrPI
BglI BglII BlnI BlpI Bpu1102I
BseAI BsiEI BsiWI BspDI BspEI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FseI FspI HindIII
KasI KspI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
StuI SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 506 GGTACC G^GTACC
AflII 1243 CTTAAG C^TTAAG
AflIII 617 ACATGT A^C[GA][CT]GT
AhdI 3040 GACGAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 867 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 506 GGTACC G^GTACC
AspEI 3040 GACGAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1492 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1270 GCTGAGG CCTCAGC(-5/-2)
BciVI 898 GGATAC GTATCC(6/5)
BclI 1700 TGATCA T^GATCA
BfaI 1936 CTAG C^TAG
BfrI 1243 CTTAAG C^TTAAG
BsaBI 168 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 895 ACCGGA [AT]^CCGG[AT]
BsgI 885 CTGCAC GTGCAG(16/14)
BsmBI 3064 GAGACG CGTCTC(1/5)
BspLU11I 617 ACATGT A^CATGT
BssSI 1903 CTCGTG CACGAG(-5/-1)
BstYI 596 AGATCC [GA]^GATC[CT]
Eam1105I 3040 GACGAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 985 ATGCAT ATGCA^T
FauI 778 GCGGG CCCGC(4/6)
FbaI 1700 TGATCA T^GATCA
HaeII 2146 GGCGCT [GA]GCGC^[CT]
KpnI 506 GGTACC GGTAC^C
MaeI 1936 CTAG C^TAG
MamI 168 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 596 AGATCC [GA]^GATC[CT]
NaeI 2498 GCCGGC GCC^GGC
NcoI 977 CCATGG C^CATGG
NgoMIV 2498 GCCGGC G^CCGGC
NsiI 985 ATGCAT ATGCA^T
PaeR7I 1913 CTCGAG C^TCGAG
PflFI 1492 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2293 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 121 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 754 CAGCTG CAG^CTG
SacI 3129 GAGCTC GAGCT^C
SbfI 2454 CCTGCAGG CCTGCA^GG
ScaI 2607 AGTACT AGT^ACT
SnaBI 2151 TACGTA TAC^GTA
SphI 2072 GCATGC GCATG^C
Sse8387I 2454 CCTGCAGG CCTGCA^GG
SspI 2638 AATATT AAT^ATT
Tth111I 1492 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2293 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1913 CTCGAG C^TCGAG
XhoII 596 AGATCC [GA]^GATC[CT]
XmnI 867 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 394 GTAGAC GT^[AC][GT]AC
2727 GTCTAC
AcyI 1958 GACGCC G[GA]^CG[CT]C
2558 GACGCC
AspHI 2288 GTGCTC G[AT]GC[AT]^C
3129 GAGCTC
AvaI 1760 CTCGGG C^[CT]CG[GA]G
1913 CTCGAG
BbsI 2854 GAAGAC GAAGAC(2/6)
2347 GTCTTC
BcgI 2705 CGACCTCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1209 GCACACCGTTCG
BpuAI 2854 GAAGAC GAAGAC(2/6)
2347 GTCTTC
BsaHI 1958 GACGCC G[GA]^CG[CT]C
2558 GACGCC
BseRI 415 CTCCTC GAGGAG(10/8)
2383 GAGGAG
BsiHKAI 2288 GTGCTC G[AT]GC[AT]^C
3129 GAGCTC
BsmI 771 GAATGC GAATGC(1/-1)
2798 GCATTC
BsoBI 1760 CTCGGG C^[CT]CG[GA]G
1913 CTCGAG
BspHI 1331 TCATGA T^CATGA
3110 TCATGA
BsrBI 1995 GAGCGG CCGCTC(-3/-3)
3138 GAGCGG
BsrFI 889 ACCGGC [GA]^CCGG[CT]
2498 GCCGGC
BstXI 1829 CCAAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2574 CCAACTCTCTGG
Cfr10I 889 ACCGGC [GA]^CCGG[CT]
2498 GCCGGC
EcoNI 93 CCTTAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
914 CCTGCGAAAGG
Hin1I 1958 GACGCC G[GA]^CG[CT]C
2558 GACGCC
MspA1I 47 CCGCTG C[AC]G^C[GT]G
754 CAGCTG
PleI 1066 GACTC GAGTC(4/5)
2941 GAGTC
PpuMI 2022 AGGACCC [GA]G^G[AT]CC[CT]
3046 GGGTCCT
PstI 1384 CTGCAG CTGCA^G
2455 CTGCAG
RcaI 1331 TCATGA T^CATGA
3110 TCATGA
SapI 3135 GAAGAGC GCTCTTC(1/4)
1984 GCTCTTC
SmlI 1243 CTTAAG C^T[CT][GA]AG
1913 CTCGAG
Tsp509I 2337 AATT ^AATT
2685 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BalI BanI BanII BbvI
BcnI BmyI BpmI BsaAI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XcmI