Restriction map for
pF1KA0744
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BciVI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraII DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRV EcoT22I FseI FspI
Hin1I HincII HindII HpaI KasI
KspI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1306 GGTACC G^GTACC
Asp718I 1306 GGTACC G^GTACC
BanI 1306 GGTACC G^G[CT][GA]CC
BbsI 1549 GAAGAC GAAGAC(2/6)
BcgI 1587 CGACAGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 11 TGATCA T^GATCA
BcnI 1116 CCCGG CC^[GC]GG
BpuAI 1549 GAAGAC GAAGAC(2/6)
BsgI 1352 CTGCAC GTGCAG(16/14)
BsmBI 1060 GAGACG CGTCTC(1/5)
BsmI 923 GCATTC GAATGC(1/-1)
BspHI 933 TCATGA T^CATGA
BspMI 950 ACCTGC ACCTGC(4/8)
BstAPI 616 GCACAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 1701 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 1558 GCGC GCG^C
DrdI 112 GACCCTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 104 CCGTGG C^C[GA][CT]GG
EarI 1519 GAAGAG CTCTTC(1/4)
EcoRI 1032 GAATTC G^AATTC
FauI 1003 CCCGC CCCGC(4/6)
FbaI 11 TGATCA T^GATCA
HaeII 1557 AGCGCC [GA]GCGC^[CT]
HapII 1117 CCGG C^CGG
HhaI 1558 GCGC GCG^C
HinP1I 1558 GCGC G^CGC
HpaII 1117 CCGG C^CGG
KpnI 1306 GGTACC GGTAC^C
Ksp632I 1519 GAAGAG CTCTTC(1/4)
MaeII 1372 ACGT A^CGT
MfeI 133 CAATTG C^AATTG
MslI 1132 CACCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1381 CAGCTG C[AC]G^C[GT]G
MspI 1117 CCGG C^CGG
MunI 133 CAATTG C^AATTG
NciI 1116 CCCGG CC^[GC]GG
PstI 1186 CTGCAG CTGCA^G
PvuII 1381 CAGCTG CAG^CTG
RcaI 933 TCATGA T^CATGA
SapI 1519 GAAGAGC GCTCTTC(1/4)
SfcI 1186 CTGCAG C^T[GA][CT]AG
SmlI 1504 CTTGAG C^T[CT][GA]AG
XcmI 983 CCAACATTACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1032 GAATTC [GA]^AATT[CT]
1286 GAATTT
AocI 75 CCTAAGG CC^T[ACGT]AGG
87 CCTCAGG
ApoI 1032 GAATTC [GA]^AATT[CT]
1286 GAATTT
Asp700I 145 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1519 GAAGAGCTTC
AspHI 614 GAGCAC G[AT]GC[AT]^C
1368 GAGCAC
BanII 55 GAGCCC G[GA]GC[CT]^C
661 GAGCCC
BlpI 241 GCTCAGC GC^T[ACGT]AGC
1378 GCTCAGC
Bpu1102I 241 GCTCAGC GC^T[ACGT]AGC
1378 GCTCAGC
BseRI 374 CTCCTC GAGGAG(10/8)
708 GAGGAG
BsiHKAI 614 GAGCAC G[AT]GC[AT]^C
1368 GAGCAC
BstYI 1442 AGATCC [GA]^GATC[CT]
1676 AGATCC
Bsu36I 75 CCTAAGG CC^T[ACGT]AGG
87 CCTCAGG
CelII 241 GCTCAGC GC^T[ACGT]AGC
1378 GCTCAGC
Eco81I 75 CCTAAGG CC^T[ACGT]AGG
87 CCTCAGG
EcoT14I 992 CCTTGG C^C[AT][AT]GG
1268 CCTTGG
HaeIII 49 GGCC GG^CC
842 GGCC
HgaI 1062 GACGC GACGC(5/10)
1068 GACGC
HindIII 436 AAGCTT A^AGCTT
1225 AAGCTT
MflI 1442 AGATCC [GA]^GATC[CT]
1676 AGATCC
MseI 688 TTAA T^TAA
1757 TTAA
StyI 992 CCTTGG C^C[AT][AT]GG
1268 CCTTGG
Tru9I 688 TTAA T^TAA
1757 TTAA
XhoII 1442 AGATCC [GA]^GATC[CT]
1676 AGATCC
XmnI 145 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1519 GAAGAGCTTC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstBI BstF5I
BstNI Cfr13I DdeI DpnII EcoNI
EcoRII Fnu4HI FokI HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV NspV PflMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfuI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I