Restriction map for
pF1KA0850
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BamHI BanI BbeI
BcgI BciVI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstYI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DrdI DsaI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HpaII KasI KpnI
KspI MfeI MflI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp45I
Tth111I Van91I XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflII 511 CTTAAG C^TTAAG
AhdI 104 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 239 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 72 CCTGAGG CC^T[ACGT]AGG
AspEI 104 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1184 GGTCC G^G[AT]CC
BbrPI 776 CACGTG CAC^GTG
BbvCI 721 GCTGAGG CCTCAGC(-5/-2)
BclI 681 TGATCA T^GATCA
BfrI 511 CTTAAG C^TTAAG
BpmI 1655 CTGGAG CTGGAG(16/14)
BsaAI 776 CACGTG [CT]AC^GT[GA]
BsiYI 565 CCCAGCAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 565 CCCAGCAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 161 GTCCC GGGAC(10/14)
BsmI 1156 GAATGC GAATGC(1/-1)
Bsp1407I 1541 TGTACA T^GTACA
BsrGI 1541 TGTACA T^GTACA
BstBI 1145 TTCGAA TT^CGAA
BstXI 1631 CCATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 72 CCTGAGG CC^T[ACGT]AGG
DraIII 773 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 104 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 72 CCTGAGG CC^T[ACGT]AGG
FbaI 681 TGATCA T^GATCA
NdeI 1418 CATATG CA^TATG
NheI 147 GCTAGC G^CTAGC
NspV 1145 TTCGAA TT^CGAA
PmaCI 776 CACGTG CAC^GTG
PmlI 776 CACGTG CAC^GTG
PshAI 1505 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 972 GAGCTC GAGCT^C
SapI 640 GAAGAGC GCTCTTC(1/4)
ScaI 985 AGTACT AGT^ACT
SfuI 1145 TTCGAA TT^CGAA
SspBI 1541 TGTACA T^GTACA
XbaI 379 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Asp700I 652 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1138 GAATGTCTTC
AspHI 972 GAGCTC G[AT]GC[AT]^C
1376 GTGCTC
BbsI 48 GTCTTC GAAGAC(2/6)
1142 GTCTTC
BpuAI 48 GTCTTC GAAGAC(2/6)
1142 GTCTTC
BsaBI 190 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
229 GATCTCAATC
BsaJI 520 CCAAGG C^C[ACGT][ACGT]GG
1783 CCAAGG
BsiHKAI 972 GAGCTC G[AT]GC[AT]^C
1376 GTGCTC
BspMI 354 GCAGGT ACCTGC(4/8)
1117 GCAGGT
BssKI 912 CCTGG ^CC[ACGT]GG
1613 CCTGG
BstNI 912 CCTGG CC^[AT]GG
1613 CCTGG
Cfr13I 1184 GGTCC G^G[ACGT]CC
1242 GGGCC
EcoRII 912 CCTGG ^CC[AT]GG
1613 CCTGG
EcoT14I 520 CCAAGG C^C[AT][AT]GG
1783 CCAAGG
HindIII 514 AAGCTT A^AGCTT
1011 AAGCTT
MaeII 215 ACGT A^CGT
777 ACGT
MamI 190 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
229 GATCTCAATC
MslI 1276 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1632 CATGAATGTG
MspA1I 396 CAGCTG C[AC]G^C[GT]G
677 CAGCTG
MvaI 912 CCTGG CC^[AT]GG
1613 CCTGG
NlaIV 701 GGAACC GG[ACGT]^[ACGT]CC
1898 GGAGCC
PvuII 396 CAGCTG CAG^CTG
677 CAGCTG
Sau96I 1184 GGTCC G^G[ACGT]CC
1242 GGGCC
ScrFI 912 CCTGG CC^[ACGT]GG
1613 CCTGG
SmlI 511 CTTAAG C^T[CT][GA]AG
1882 CTTGAG
StyI 520 CCAAGG C^C[AT][AT]GG
1783 CCAAGG
XcmI 1715 CCATCAGTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1820 CCATTTATGCAGTGG
XmnI 652 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1138 GAATGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BalI BanII BbvI BfaI BmyI
BseRI BsgI BsmAI Bsp1286I BsrDI
BsrI BstF5I Cac8I DdeI DpnII
EaeI EarI Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MwoI NdeII NlaIII
PleI PstI RsaI Sau3AI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I