Restriction map for
pF1KA0877
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstUI BstXI
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV FseI FspI HaeII HapII
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MamI MluI MluNI MroI
MscI MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI TaqI TthHB8I Van91I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AspI 56 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1291 GGTGCC G^G[CT][GA]CC
BbsI 1360 GAAGAC GAAGAC(2/6)
BclI 969 TGATCA T^GATCA
BpuAI 1360 GAAGAC GAAGAC(2/6)
BseRI 47 GAGGAG GAGGAG(10/8)
BsiYI 1547 CCAATGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1547 CCAATGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BstAPI 1279 GCAGTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 39 GGTTACC G^GT[ACGT]ACC
BstPI 39 GGTTACC G^GT[ACGT]ACC
Cfr13I 1334 GGCCC G^G[ACGT]CC
DraI 126 TTTAAA TTT^AAA
EcoO65I 39 GGTTACC G^GT[ACGT]ACC
EcoT22I 992 ATGCAT ATGCA^T
FauI 1288 GCGGG CCCGC(4/6)
FbaI 969 TGATCA T^GATCA
MfeI 1000 CAATTG C^AATTG
MspA1I 806 CAGCGG C[AC]G^C[GT]G
MunI 1000 CAATTG C^AATTG
NlaIV 1291 GGTGCC GG[ACGT]^[ACGT]CC
NsiI 992 ATGCAT ATGCA^T
PflFI 56 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Sau96I 1334 GGCCC G^G[ACGT]CC
ScaI 1264 AGTACT AGT^ACT
SspI 1204 AATATT AAT^ATT
StuI 192 AGGCCT AGG^CCT
Tsp45I 310 GTGAC ^GT[GC]AC
Tth111I 56 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1470 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 795 GTATAC GT^[AC][GT]AC
1393 GTATAC
AciI 808 GCGG CCGC(-3/-1)
1288 GCGG
AflIII 948 ACATGT A^C[GA][CT]GT
1064 ACATGT
BsaJI 391 CCTTGG C^C[ACGT][ACGT]GG
1020 CCTTGG
BsgI 1325 CTGCAC GTGCAG(16/14)
803 GTGCAG
BsmAI 832 GAGAC GTCTC(1/5)
1038 GAGAC
BspLU11I 948 ACATGT A^CATGT
1064 ACATGT
Bst1107I 795 GTATAC GTA^TAC
1393 GTATAC
BstZ17I 795 GTATAC GTA^TAC
1393 GTATAC
Cac8I 97 GCTTGC GC[ACGT]^[ACGT]GC
1366 GCAGGC
EarI 1048 CTCTTC CTCTTC(1/4)
1474 GAAGAG
EcoT14I 391 CCTTGG C^C[AT][AT]GG
1020 CCTTGG
HaeIII 193 GGCC GG^CC
1334 GGCC
HgaI 730 GACGC GACGC(5/10)
1363 GACGC
HindIII 270 AAGCTT A^AGCTT
348 AAGCTT
HphI 1581 GGTGA GGTGA(8/7)
1604 TCACC
Ksp632I 1048 CTCTTC CTCTTC(1/4)
1474 GAAGAG
NspI 948 ACATGT [GA]CATG^[CT]
1064 ACATGT
SmlI 1092 CTCAAG C^T[CT][GA]AG
1371 CTTGAG
StyI 391 CCTTGG C^C[AT][AT]GG
1020 CCTTGG
TfiI 1344 GAATC G^A[AT]TC
1588 GATTC
XcmI 963 CCAGTTTGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1295 CCATGCCCACGATGG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
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AcsI AfaI AluI AlwI ApoI
BbvI BfaI BsrDI BsrI BssKI
BstF5I BstNI BstYI DdeI DpnII
EcoRII Fnu4HI FokI HinfI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII PleI RsaI Sau3AI
ScrFI SfaNI Tru9I TseI Tsp509I
TspRI XhoII