Restriction map for
pF1KA0906
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# NO CUT ENZYMES ( 54 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI AscI Asp718I AspEI AviII
BfrI BglII BsaWI BseAI BsiWI
BspDI BspEI BsrBI BssHII Bst1107I
BstBI BstZ17I ClaI DraI DraIII
Eam1105I EcoRI EcoRV FseI FspI
HindIII KpnI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NspV PacI PinAI PmeI Psp1406I
PvuI SalI SbfI SfuI SgrAI
SpeI Sse8387I SwaI XbaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Aor51HI 3367 AGCGCT AGC^GCT
AseI 3894 ATTAAT AT^TAAT
AsnI 3894 ATTAAT AT^TAAT
AspI 33 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1041 CCTAGG C^CTAGG
BbeI 1408 GGCGCC GGCGC^C
BbrPI 1640 CACGTG CAC^GTG
BclI 5471 TGATCA T^GATCA
BlnI 1041 CCTAGG C^CTAGG
BlpI 2461 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2461 GCTGAGC GC^T[ACGT]AGC
BsaBI 3552 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 5194 GCATTC GAATGC(1/-1)
Bsp1407I 2942 TGTACA T^GTACA
BspLU11I 2291 ACATGT A^CATGT
BsrGI 2942 TGTACA T^GTACA
CelII 2461 GCTGAGC GC^T[ACGT]AGC
CpoI 3748 CGGACCG CG^G[AT]CCG
DrdI 1738 GACTTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3367 AGCGCT AGC^GCT
EcoT22I 5594 ATGCAT ATGCA^T
FbaI 5471 TGATCA T^GATCA
HpaI 127 GTTAAC GTT^AAC
KasI 1408 GGCGCC G^GCGCC
MamI 3552 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 4848 GCCGGC GCC^GGC
NarI 1408 GGCGCC GG^CGCC
NgoMIV 4848 GCCGGC G^CCGGC
NsiI 5594 ATGCAT ATGCA^T
PflFI 33 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1640 CACGTG CAC^GTG
PmlI 1640 CACGTG CAC^GTG
PshBI 3894 ATTAAT AT^TAAT
RsrII 3748 CGGACCG CG^G[AT]CCG
SapI 5439 GCTCTTC GCTCTTC(1/4)
ScaI 4919 AGTACT AGT^ACT
SfiI 4173 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1408 GGCGCC GGC^GCC
SnaBI 555 TACGTA TAC^GTA
SphI 983 GCATGC GCATG^C
SspBI 2942 TGTACA T^GTACA
SspI 3891 AATATT AAT^ATT
Tth111I 33 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AatII 748 GACGTC GACGT^C
3652 GACGTC
AcsI 1346 AAATTC [GA]^AATT[CT]
2029 AAATTC
Alw44I 2836 GTGCAC G^TGCAC
4773 GTGCAC
ApaLI 2836 GTGCAC G^TGCAC
4773 GTGCAC
ApoI 1346 AAATTC [GA]^AATT[CT]
2029 AAATTC
BspHI 2867 TCATGA T^CATGA
5468 TCATGA
HaeII 1408 GGCGCC [GA]GCGC^[CT]
3367 AGCGCT
HincII 127 GTTAAC GT[CT]^[GA]AC
2956 GTTGAC
HindII 127 GTTAAC GT[CT]^[GA]AC
2956 GTTGAC
KspI 3138 CCGCGG CCGC^GG
5261 CCGCGG
MseI 128 TTAA T^TAA
3895 TTAA
NcoI 2831 CCATGG C^CATGG
4454 CCATGG
PaeR7I 2017 CTCGAG C^TCGAG
5534 CTCGAG
PshAI 3695 GACTCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5460 GACAGCGGTC
RcaI 2867 TCATGA T^CATGA
5468 TCATGA
SacII 3138 CCGCGG CCGC^GG
5261 CCGCGG
SexAI 1475 ACCTGGT A^CC[AT]GGT
5420 ACCAGGT
Tru9I 128 TTAA T^TAA
3895 TTAA
XhoI 2017 CTCGAG C^TCGAG
5534 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI Asp700I AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcgI BciVI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI