Restriction map for
pF1KA0914
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccI AccII AclI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsgI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI Bst1107I BstUI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DsaI Eam1105I Eco47III
EcoNI EcoRI EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KspI MaeI MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1532 GGTACC G^GTACC
AcsI 1162 AAATTC [GA]^AATT[CT]
AcyI 824 GGCGCC G[GA]^CG[CT]C
Alw44I 1658 GTGCAC G^TGCAC
AocI 1390 CCTGAGG CC^T[ACGT]AGG
ApaLI 1658 GTGCAC G^TGCAC
ApoI 1162 AAATTC [GA]^AATT[CT]
Asp718I 1532 GGTACC G^GTACC
AvaI 1559 CCCGAG C^[CT]CG[GA]G
BanII 600 GAGCCC G[GA]GC[CT]^C
BbeI 824 GGCGCC GGCGC^C
BcgI 1650 CGATTTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 824 GGCGCC G[GA]^CG[CT]C
BsiEI 1483 CGGCCG CG[GA][CT]^CG
BsmFI 294 GGGAC GGGAC(10/14)
BsmI 1472 GCATTC GAATGC(1/-1)
BsoBI 1559 CCCGAG C^[CT]CG[GA]G
BsrBI 1373 GAGCGG CCGCTC(-3/-3)
BstAPI 217 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 670 GGTGACC G^GT[ACGT]ACC
BstPI 670 GGTGACC G^GT[ACGT]ACC
Bsu36I 1390 CCTGAGG CC^T[ACGT]AGG
DrdI 1627 GACTTCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1483 CGGCCG [CT]^GGCC[GA]
EagI 1483 CGGCCG C^GGCCG
EclXI 1483 CGGCCG C^GGCCG
Eco52I 1483 CGGCCG C^GGCCG
Eco81I 1390 CCTGAGG CC^T[ACGT]AGG
EcoO65I 670 GGTGACC G^GT[ACGT]ACC
EcoRV 1197 GATATC GAT^ATC
EcoT14I 257 CCAAGG C^C[AT][AT]GG
FauI 777 GCGGG CCCGC(4/6)
HaeIII 1484 GGCC GG^CC
HgaI 1690 GACGC GACGC(5/10)
Hin1I 824 GGCGCC G[GA]^CG[CT]C
KasI 824 GGCGCC G^GCGCC
KpnI 1532 GGTACC GGTAC^C
MaeII 423 ACGT A^CGT
NarI 824 GGCGCC GG^CGCC
PshAI 47 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1444 CTGCAG CTGCA^G
SapI 745 GAAGAGC GCTCTTC(1/4)
ScaI 436 AGTACT AGT^ACT
SfoI 824 GGCGCC GGC^GCC
SmlI 1270 CTTGAG C^T[CT][GA]AG
StyI 257 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwNI 197 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
838 CAGCTGCTG
Asp700I 1342 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1468 GAAAGCATTC
BanI 824 GGCGCC G^G[CT][GA]CC
1532 GGTACC
BspMI 1014 ACCTGC ACCTGC(4/8)
1506 GCAGGT
BsrDI 92 GCAATG GCAATG(2/0)
1613 GCAATG
CfoI 243 GCGC GCG^C
825 GCGC
HaeII 242 AGCGCC [GA]GCGC^[CT]
824 GGCGCC
HhaI 243 GCGC GCG^C
825 GCGC
HinP1I 243 GCGC G^CGC
825 GCGC
PleI 193 GACTC GAGTC(4/5)
790 GACTC
PvuII 137 CAGCTG CAG^CTG
838 CAGCTG
SfcI 140 CTGTAG C^T[GA][CT]AG
1444 CTGCAG
XmnI 1342 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1468 GAAAGCATTC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AspHI AvaII BbsI BbvI BmyI
BpmI BpuAI BsaJI BsaWI BseAI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspEI BspHI BsrFI BsrI
BssKI BstBI BstF5I BstNI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MroI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspV PpuMI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfuI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII