Restriction map for
pF1KA0926
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII Acc65I AccIII AflII Aor51HI
AscI AseI AsnI Asp718I AspI
AviII AvrII BanI BbeI BcgI
BcnI BfrI BlnI BsaBI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FseI FspI HaeII HpaI
KasI KpnI KspI MamI MluI
MroI NarI NciI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PmeI PshAI PshBI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 1652 GTCTAC GT^[AC][GT]AC
AccII 3718 CGCG CG^CG
AclI 2184 AACGTT AA^CGTT
AflIII 2011 ACACGT A^C[GA][CT]GT
AgeI 3156 ACCGGT A^CCGGT
AhdI 493 GACCATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 493 GACCATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2287 CACGTG CAC^GTG
BclI 1364 TGATCA T^GATCA
BsaAI 2287 CACGTG [CT]AC^GT[GA]
BsmBI 2496 GAGACG CGTCTC(1/5)
BsrDI 1894 GCAATG GCAATG(2/0)
BssSI 4128 CACGAG CACGAG(-5/-1)
BstBI 2212 TTCGAA TT^CGAA
BstUI 3718 CGCG CG^CG
BstXI 1062 CCAGCTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 3559 TTTAAA TTT^AAA
Eam1105I 493 GACCATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3382 GAATTC G^AATTC
FbaI 1364 TGATCA T^GATCA
HindIII 2296 AAGCTT A^AGCTT
MfeI 3116 CAATTG C^AATTG
MunI 3116 CAATTG C^AATTG
MvnI 3718 CGCG CG^CG
NdeI 1976 CATATG CA^TATG
NspV 2212 TTCGAA TT^CGAA
PinAI 3156 ACCGGT A^CCGGT
PmaCI 2287 CACGTG CAC^GTG
PmlI 2287 CACGTG CAC^GTG
Psp1406I 2184 AACGTT AA^CGTT
SexAI 167 ACCTGGT A^CC[AT]GGT
SfuI 2212 TTCGAA TT^CGAA
SspI 1463 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcyI 434 GACGCC G[GA]^CG[CT]C
2498 GACGCC
AocI 1770 CCTCAGG CC^T[ACGT]AGG
3727 CCTGAGG
Asp700I 63 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4149 GAAGCTGTTC
AvaI 1348 CCCGAG C^[CT]CG[GA]G
4040 CCCGAG
BalI 1028 TGGCCA TGG^CCA
3843 TGGCCA
BamHI 359 GGATCC G^GATCC
559 GGATCC
BciVI 966 GGATAC GTATCC(6/5)
2994 GGATAC
BglI 1168 GCCACTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1203 GCCAGAGCGGC
BglII 3107 AGATCT A^GATCT
3927 AGATCT
BsaHI 434 GACGCC G[GA]^CG[CT]C
2498 GACGCC
BsaWI 3156 ACCGGT [AT]^CCGG[AT]
4178 ACCGGA
BsoBI 1348 CCCGAG C^[CT]CG[GA]G
4040 CCCGAG
BsrBI 125 CCGCTC CCGCTC(-3/-3)
1207 GAGCGG
BstEII 2415 GGTCACC G^GT[ACGT]ACC
3366 GGTGACC
BstPI 2415 GGTCACC G^GT[ACGT]ACC
3366 GGTGACC
Bsu36I 1770 CCTCAGG CC^T[ACGT]AGG
3727 CCTGAGG
DraIII 3502 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
4006 CACTTTGTG
EaeI 1028 TGGCCA [CT]^GGCC[GA]
3843 TGGCCA
Eco81I 1770 CCTCAGG CC^T[ACGT]AGG
3727 CCTGAGG
EcoO65I 2415 GGTCACC G^GT[ACGT]ACC
3366 GGTGACC
Hin1I 434 GACGCC G[GA]^CG[CT]C
2498 GACGCC
HincII 1020 GTCAAC GT[CT]^[GA]AC
3271 GTTGAC
HindII 1020 GTCAAC GT[CT]^[GA]AC
3271 GTTGAC
MluNI 1028 TGGCCA TGG^CCA
3843 TGGCCA
MscI 1028 TGGCCA TGG^CCA
3843 TGGCCA
NspI 1086 GCATGT [GA]CATG^[CT]
2225 GCATGT
PflMI 2733 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3399 CCAGTTCCTGG
StuI 960 AGGCCT AGG^CCT
3908 AGGCCT
TfiI 918 GAATC G^A[AT]TC
2394 GATTC
Van91I 2733 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3399 CCAGTTCCTGG
XmnI 63 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4149 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaI ApaLI ApoI
AspHI AvaII BanII BbsI BbvCI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NcoI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII