Restriction map for
pF1KA0930
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BamHI BbeI BbvCI BciVI BclI
BfrI BglII BlnI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstXI BstYI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I TspRI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 810 GTCTAC GT^[AC][GT]AC
AccII 450 CGCG CG^CG
Alw44I 390 GTGCAC G^TGCAC
AlwI 971 GGATC GGATC(4/5)
AlwNI 358 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 253 CCTGAGG CC^T[ACGT]AGG
ApaI 765 GGGCCC GGGCC^C
ApaLI 390 GTGCAC G^TGCAC
AspHI 390 GTGCAC G[AT]GC[AT]^C
AviII 755 TGCGCA TGC^GCA
BalI 23 TGGCCA TGG^CCA
BanI 957 GGTGCC G^G[CT][GA]CC
BbrPI 395 CACGTG CAC^GTG
BbsI 115 GTCTTC GAAGAC(2/6)
BcgI 219 CGAAAGCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 599 CTAG C^TAG
BlpI 247 GCTGAGC GC^T[ACGT]AGC
BpmI 849 CTCCAG CTGGAG(16/14)
Bpu1102I 247 GCTGAGC GC^T[ACGT]AGC
BpuAI 115 GTCTTC GAAGAC(2/6)
BsaAI 395 CACGTG [CT]AC^GT[GA]
BsaWI 968 ACCGGA [AT]^CCGG[AT]
BsiHKAI 390 GTGCAC G[AT]GC[AT]^C
BspHI 521 TCATGA T^CATGA
BspMI 1064 ACCTGC ACCTGC(4/8)
BsrBI 874 GAGCGG CCGCTC(-3/-3)
BssSI 871 CACGAG CACGAG(-5/-1)
BstUI 450 CGCG CG^CG
Bsu36I 253 CCTGAGG CC^T[ACGT]AGG
CelII 247 GCTGAGC GC^T[ACGT]AGC
DrdI 106 GACCGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 470 CCATGG C^C[GA][CT]GG
EarI 841 GAAGAG CTCTTC(1/4)
Eco81I 253 CCTGAGG CC^T[ACGT]AGG
FspI 755 TGCGCA TGC^GCA
HaeII 657 GGCGCT [GA]GCGC^[CT]
Ksp632I 841 GAAGAG CTCTTC(1/4)
MaeI 599 CTAG C^TAG
MluNI 23 TGGCCA TGG^CCA
MscI 23 TGGCCA TGG^CCA
MslI 1227 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 450 CGCG CG^CG
NcoI 470 CCATGG C^CATGG
NspI 176 ACATGC [GA]CATG^[CT]
PmaCI 395 CACGTG CAC^GTG
PmlI 395 CACGTG CAC^GTG
PpuMI 1108 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 245 CAGCTG CAG^CTG
RcaI 521 TCATGA T^CATGA
SfiI 44 GGCCAGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 300 AGGCCT AGG^CCT
TfiI 345 GAATC G^A[AT]TC
XcmI 137 CCACCTACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AflIII 394 ACACGT A^C[GA][CT]GT
614 ACACGT
BsaI 308 GAGACC GGTCTC(1/5)
1070 GGTCTC
BsgI 1048 CTGCAC GTGCAG(16/14)
227 GTGCAG
BsmAI 308 GAGAC GTCTC(1/5)
1071 GTCTC
BsrFI 275 ACCGGC [GA]^CCGG[CT]
920 ACCGGC
BsrI 323 ACTGG ACTGG(1/-1)
458 CCAGT
BstEII 375 GGTGACC G^GT[ACGT]ACC
879 GGTGACC
BstPI 375 GGTGACC G^GT[ACGT]ACC
879 GGTGACC
Cfr10I 275 ACCGGC [GA]^CCGG[CT]
920 ACCGGC
EcoO65I 375 GGTGACC G^GT[ACGT]ACC
879 GGTGACC
EcoT14I 26 CCAAGG C^C[AT][AT]GG
470 CCATGG
HgaI 335 GCGTC GACGC(5/10)
451 GCGTC
MseI 1162 TTAA T^TAA
1195 TTAA
MspA1I 245 CAGCTG C[AC]G^C[GT]G
1180 CCGCTG
PstI 86 CTGCAG CTGCA^G
355 CTGCAG
SfaNI 110 GCATC GCATC(5/9)
1185 GCATC
SmlI 661 CTCAAG C^T[CT][GA]AG
946 CTCAAG
StyI 26 CCAAGG C^C[AT][AT]GG
470 CCATGG
TaqI 320 TCGA T^CGA
536 TCGA
Tru9I 1162 TTAA T^TAA
1195 TTAA
TthHB8I 320 TCGA T^CGA
536 TCGA
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BcnI BglI BmyI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfcI TseI Tsp45I