Restriction map for
pF1KA0938
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# NO CUT ENZYMES ( 68 enzymes) #
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AclI Aor51HI AscI AseI AsnI
AvrII BalI BbrPI BcgI BlnI
BsaAI BsaBI BsiEI BsiWI Bsp1407I
BspDI BsrGI BssHII BstAPI BstEII
BstPI ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI HgaI HpaI KspI
MamI MluI MluNI MscI NaeI
NdeI NgoMIV NotI NruI NsiI
PacI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SbfI ScaI SexAI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 4244 GACGTC GACGT^C
Acc65I 799 GGTACC G^GTACC
AccIII 29 TCCGGA T^CCGGA
AflII 2472 CTTAAG C^TTAAG
AgeI 3920 ACCGGT A^CCGGT
AhdI 4645 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2935 GGGCCC GGGCC^C
Asp700I 3082 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 799 GGTACC G^GTACC
AspEI 4645 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 128 TGCGCA TGC^GCA
BbeI 1494 GGCGCC GGCGC^C
BfrI 2472 CTTAAG C^TTAAG
BseAI 29 TCCGGA T^CCGGA
BsmBI 4246 CGTCTC CGTCTC(1/5)
BspEI 29 TCCGGA T^CCGGA
BspHI 2699 TCATGA T^CATGA
BspMI 2266 GCAGGT ACCTGC(4/8)
BsrBI 190 GAGCGG CCGCTC(-3/-3)
BsrDI 4311 CATTGC GCAATG(2/0)
BsrFI 3920 ACCGGT [GA]^CCGG[CT]
Bst1107I 840 GTATAC GTA^TAC
BstZ17I 840 GTATAC GTA^TAC
Cfr10I 3920 ACCGGT [GA]^CCGG[CT]
DraII 3830 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1111 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4645 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1948 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3830 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 3378 GAATTC G^AATTC
FspI 128 TGCGCA TGC^GCA
KasI 1494 GGCGCC G^GCGCC
KpnI 799 GGTACC GGTAC^C
MroI 29 TCCGGA T^CCGGA
NarI 1494 GGCGCC GG^CGCC
NheI 4315 GCTAGC G^CTAGC
PflMI 716 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3920 ACCGGT A^CCGGT
RcaI 2699 TCATGA T^CATGA
SalI 704 GTCGAC G^TCGAC
SfiI 1951 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1494 GGCGCC GGC^GCC
Van91I 716 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3082 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccI 704 GTCGAC GT^[AC][GT]AC
840 GTATAC
AcyI 1494 GGCGCC G[GA]^CG[CT]C
4244 GACGTC
Alw44I 1523 GTGCAC G^TGCAC
2186 GTGCAC
ApaLI 1523 GTGCAC G^TGCAC
2186 GTGCAC
BamHI 2209 GGATCC G^GATCC
4189 GGATCC
BclI 1733 TGATCA T^GATCA
3855 TGATCA
BsaHI 1494 GGCGCC G[GA]^CG[CT]C
4244 GACGTC
BspLU11I 884 ACATGT A^CATGT
929 ACATGT
BstBI 2243 TTCGAA TT^CGAA
4946 TTCGAA
FbaI 1733 TGATCA T^GATCA
3855 TGATCA
Hin1I 1494 GGCGCC G[GA]^CG[CT]C
4244 GACGTC
HincII 704 GTCGAC GT[CT]^[GA]AC
2080 GTCAAC
HindII 704 GTCGAC GT[CT]^[GA]AC
2080 GTCAAC
MfeI 4625 CAATTG C^AATTG
4797 CAATTG
MunI 4625 CAATTG C^AATTG
4797 CAATTG
NspV 2243 TTCGAA TT^CGAA
4946 TTCGAA
SfuI 2243 TTCGAA TT^CGAA
4946 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AspI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BciVI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
Eco81I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PaeR7I PflFI
PleI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI