Restriction map for
pF1KA0949
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI AscI AseI AsnI Asp718I
AspI AvrII BalI BamHI BcgI
BglII BlnI BsaBI BsiWI BsmBI
BspDI BspLU11I BsrDI BssHII Bst1107I
BstBI BstZ17I ClaI DraI DsaI
EcoT22I FseI HpaI KpnI KspI
MamI MfeI MluI MluNI MscI
MunI NcoI NdeI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
SacII SalI SfiI SfuI SgrAI
SnaBI SpeI SwaI Tth111I
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 3728 TCCGGA T^CCGGA
AflII 2614 CTTAAG C^TTAAG
AhdI 411 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4193 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3212 AGCGCT AGC^GCT
AspEI 411 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4451 TGCGCA TGC^GCA
BbeI 1537 GGCGCC GGCGC^C
BclI 899 TGATCA T^GATCA
BfrI 2614 CTTAAG C^TTAAG
BglI 1276 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 3728 TCCGGA T^CCGGA
Bsp1407I 1554 TGTACA T^GTACA
BspEI 3728 TCCGGA T^CCGGA
BsrGI 1554 TGTACA T^GTACA
BstEII 3219 GGTCACC G^GT[ACGT]ACC
BstPI 3219 GGTCACC G^GT[ACGT]ACC
Bsu36I 4193 CCTCAGG CC^T[ACGT]AGG
DraIII 2676 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4590 GACCCCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4500 CGGCCG C^GGCCG
Eam1105I 411 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4500 CGGCCG C^GGCCG
Eco47III 3212 AGCGCT AGC^GCT
Eco52I 4500 CGGCCG C^GGCCG
Eco81I 4193 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3219 GGTCACC G^GT[ACGT]ACC
EcoRV 223 GATATC GAT^ATC
FbaI 899 TGATCA T^GATCA
FspI 4451 TGCGCA TGC^GCA
HincII 1269 GTTGAC GT[CT]^[GA]AC
HindII 1269 GTTGAC GT[CT]^[GA]AC
HindIII 2010 AAGCTT A^AGCTT
KasI 1537 GGCGCC G^GCGCC
MroI 3728 TCCGGA T^CCGGA
NaeI 4565 GCCGGC GCC^GGC
NarI 1537 GGCGCC GG^CGCC
NgoMIV 4565 GCCGGC G^CCGGC
NheI 3186 GCTAGC G^CTAGC
PaeR7I 3838 CTCGAG C^TCGAG
ScaI 3953 AGTACT AGT^ACT
SexAI 3362 ACCAGGT A^CC[AT]GGT
SfoI 1537 GGCGCC GGC^GCC
SspBI 1554 TGTACA T^GTACA
SspI 2635 AATATT AAT^ATT
XcmI 2543 CCATGAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3838 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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ApaI 4179 GGGCCC GGGCC^C
4293 GGGCCC
Asp700I 2197 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2386 GAAGCCCTTC
BbrPI 1653 CACGTG CAC^GTG
2769 CACGTG
BlpI 496 GCTGAGC GC^T[ACGT]AGC
4509 GCTCAGC
Bpu1102I 496 GCTGAGC GC^T[ACGT]AGC
4509 GCTCAGC
BsaWI 2414 TCCGGT [AT]^CCGG[AT]
3728 TCCGGA
BsiEI 2518 CGGTCG CG[GA][CT]^CG
4500 CGGCCG
BspHI 2220 TCATGA T^CATGA
3491 TCATGA
BsrFI 3021 GCCGGT [GA]^CCGG[CT]
4565 GCCGGC
CelII 496 GCTGAGC GC^T[ACGT]AGC
4509 GCTCAGC
Cfr10I 3021 GCCGGT [GA]^CCGG[CT]
4565 GCCGGC
CpoI 2416 CGGTCCG CG^G[AT]CCG
4441 CGGACCG
EaeI 4470 TGGCCG [CT]^GGCC[GA]
4500 CGGCCG
EcoRI 394 GAATTC G^AATTC
3844 GAATTC
EcoT14I 2867 CCAAGG C^C[AT][AT]GG
3869 CCTTGG
MslI 1384 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2723 CATCCAAATG
PmaCI 1653 CACGTG CAC^GTG
2769 CACGTG
PmlI 1653 CACGTG CAC^GTG
2769 CACGTG
RcaI 2220 TCATGA T^CATGA
3491 TCATGA
RsrII 2416 CGGTCCG CG^G[AT]CCG
4441 CGGACCG
SbfI 1248 CCTGCAGG CCTGCA^GG
1278 CCTGCAGG
SphI 1983 GCATGC GCATG^C
2624 GCATGC
Sse8387I 1248 CCTGCAGG CCTGCA^GG
1278 CCTGCAGG
StyI 2867 CCAAGG C^C[AT][AT]GG
3869 CCTTGG
XmnI 2197 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2386 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII
XmaCI XmaI