Restriction map for
pF1KA0953
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BbrPI
BcgI BclI BfrI BglI BglII
BlnI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspLU11I BsrBI BsrGI Bst1107I
BstAPI BstEII BstPI BstZ17I DraIII
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MfeI MluI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI SalI SapI SfiI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 1756 GTCTAC GT^[AC][GT]AC
AccIII 2127 TCCGGA T^CCGGA
AflIII 215 ACGTGT A^C[GA][CT]GT
AhdI 391 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 70 CCTGAGG CC^T[ACGT]AGG
ApaI 985 GGGCCC GGGCC^C
AspEI 391 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1540 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 308 TGGCCA TGG^CCA
BamHI 75 GGATCC G^GATCC
BbeI 160 GGCGCC GGCGC^C
BciVI 1312 GTATCC GTATCC(6/5)
BlpI 1032 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1032 GCTCAGC GC^T[ACGT]AGC
BsaAI 214 TACGTG [CT]AC^GT[GA]
BseAI 2127 TCCGGA T^CCGGA
BspDI 203 ATCGAT AT^CGAT
BspEI 2127 TCCGGA T^CCGGA
BsrDI 222 CATTGC GCAATG(2/0)
BssHII 924 GCGCGC G^CGCGC
BssSI 1105 CACGAG CACGAG(-5/-1)
BstBI 488 TTCGAA TT^CGAA
Bsu36I 70 CCTGAGG CC^T[ACGT]AGG
CelII 1032 GCTCAGC GC^T[ACGT]AGC
ClaI 203 ATCGAT AT^CGAT
CpoI 2012 CGGACCG CG^G[AT]CCG
DraI 823 TTTAAA TTT^AAA
Eam1105I 391 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 70 CCTGAGG CC^T[ACGT]AGG
EcoT14I 800 CCAAGG C^C[AT][AT]GG
HgaI 895 GACGC GACGC(5/10)
HincII 55 GTTGAC GT[CT]^[GA]AC
HindII 55 GTTGAC GT[CT]^[GA]AC
KasI 160 GGCGCC G^GCGCC
MluNI 308 TGGCCA TGG^CCA
MroI 2127 TCCGGA T^CCGGA
MscI 308 TGGCCA TGG^CCA
MseI 824 TTAA T^TAA
NarI 160 GGCGCC GG^CGCC
NspV 488 TTCGAA TT^CGAA
PaeR7I 453 CTCGAG C^TCGAG
PflFI 1540 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 190 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2012 CGGACCG CG^G[AT]CCG
SacI 1954 GAGCTC GAGCT^C
ScaI 993 AGTACT AGT^ACT
SexAI 863 ACCTGGT A^CC[AT]GGT
SfoI 160 GGCGCC GGC^GCC
SfuI 488 TTCGAA TT^CGAA
StuI 507 AGGCCT AGG^CCT
StyI 800 CCAAGG C^C[AT][AT]GG
Tru9I 824 TTAA T^TAA
Tth111I 1540 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1422 TCTAGA T^CTAGA
XhoI 453 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 485 AAATTC [GA]^AATT[CT]
1406 AAATTC
AcyI 160 GGCGCC G[GA]^CG[CT]C
895 GACGCC
AlwNI 877 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1823 CAGTGCCTG
Aor51HI 910 AGCGCT AGC^GCT
1178 AGCGCT
ApoI 485 AAATTC [GA]^AATT[CT]
1406 AAATTC
BbvCI 2038 CCTCAGC CCTCAGC(-5/-2)
1017 GCTGAGG
BcnI 723 CCGGG CC^[GC]GG
1055 CCGGG
BfaI 1355 CTAG C^TAG
1423 CTAG
BsaBI 1428 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2050 GATGAGGATC
BsaHI 160 GGCGCC G[GA]^CG[CT]C
895 GACGCC
BsaWI 407 ACCGGA [AT]^CCGG[AT]
2127 TCCGGA
BsgI 1228 CTGCAC GTGCAG(16/14)
1615 GTGCAG
BspHI 1202 TCATGA T^CATGA
1550 TCATGA
BsrI 996 ACTGG ACTGG(1/-1)
1326 CCAGT
BstYI 75 GGATCC [GA]^GATC[CT]
344 AGATCC
DrdI 1066 GACGGGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1495 GACATCTCTGTC
EaeI 308 TGGCCA [CT]^GGCC[GA]
1219 CGGCCA
Eco47III 910 AGCGCT AGC^GCT
1178 AGCGCT
Hin1I 160 GGCGCC G[GA]^CG[CT]C
895 GACGCC
MaeI 1355 CTAG C^TAG
1423 CTAG
MaeIII 188 GTGAC ^GT[ACGT]AC
1493 GTGAC
MamI 1428 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2050 GATGAGGATC
MflI 75 GGATCC [GA]^GATC[CT]
344 AGATCC
NciI 723 CCGGG CC^[GC]GG
1055 CCGGG
PflMI 554 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1233 CCAGGCGGTGG
PleI 1412 GACTC GAGTC(4/5)
322 GAGTC
PpuMI 563 GGGACCC [GA]G^G[AT]CC[CT]
1211 AGGTCCC
RcaI 1202 TCATGA T^CATGA
1550 TCATGA
SbfI 1305 CCTGCAGG CCTGCA^GG
2097 CCTGCAGG
SmlI 298 CTCAAG C^T[CT][GA]AG
453 CTCGAG
Sse8387I 1305 CCTGCAGG CCTGCA^GG
2097 CCTGCAGG
Tsp45I 188 GTGAC ^GT[GC]AC
1493 GTGAC
Van91I 554 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1233 CCAGGCGGTGG
XcmI 971 CCACCGGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1382 CCACCGCCCTCATGG
XhoII 75 GGATCC [GA]^GATC[CT]
344 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EarI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI TseI Tsp509I
TspRI TthHB8I