Restriction map for
pF1KA0973
##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AclI AflIII AgeI AhdI AseI
AsnI AspEI BciVI BclI BglII
BsaBI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrDI BsrGI Bst1107I BstZ17I
ClaI CpoI DraI Eam1105I FbaI
FseI HincII HindII HindIII HpaI
MamI MfeI MluI MunI NdeI
NotI NruI PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 4153 GACGTC GACGT^C
Acc65I 4299 GGTACC G^GTACC
AcsI 877 GAATTC [GA]^AATT[CT]
AflII 3121 CTTAAG C^TTAAG
AocI 3100 CCTGAGG CC^T[ACGT]AGG
ApoI 877 GAATTC [GA]^AATT[CT]
AscI 3961 GGCGCGCC GG^CGCGCC
Asp700I 1113 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4299 GGTACC G^GTACC
AspI 696 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2491 GGATCC G^GATCC
BfrI 3121 CTTAAG C^TTAAG
BstBI 3160 TTCGAA TT^CGAA
Bsu36I 3100 CCTGAGG CC^T[ACGT]AGG
DrdI 2968 GACACGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3100 CCTGAGG CC^T[ACGT]AGG
EcoRI 877 GAATTC G^AATTC
EcoRV 1270 GATATC GAT^ATC
EcoT22I 2615 ATGCAT ATGCA^T
KpnI 4299 GGTACC GGTAC^C
KspI 2486 CCGCGG CCGC^GG
MseI 3122 TTAA T^TAA
NheI 1446 GCTAGC G^CTAGC
NsiI 2615 ATGCAT ATGCA^T
NspV 3160 TTCGAA TT^CGAA
PflFI 696 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 385 GAGCTC GAGCT^C
SacII 2486 CCGCGG CCGC^GG
SexAI 1166 ACCTGGT A^CC[AT]GGT
SfiI 2462 GGCCTCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3160 TTCGAA TT^CGAA
SgrAI 259 CGCCGGTG C[GA]^CCGG[CT]G
SspI 1393 AATATT AAT^ATT
Tru9I 3122 TTAA T^TAA
Tth111I 696 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1113 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 2310 TCCGGA T^CCGGA
3284 TCCGGA
AlwNI 2741 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
4351 CAGCCTCTG
ApaI 2229 GGGCCC GGGCC^C
2507 GGGCCC
AvrII 168 CCTAGG C^CTAGG
3447 CCTAGG
BalI 4208 TGGCCA TGG^CCA
4220 TGGCCA
BbrPI 2050 CACGTG CAC^GTG
3064 CACGTG
BbsI 948 GAAGAC GAAGAC(2/6)
2289 GAAGAC
BcgI 3305 CGAACCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3516 GCACCACATTCG
BlnI 168 CCTAGG C^CTAGG
3447 CCTAGG
BpuAI 948 GAAGAC GAAGAC(2/6)
2289 GAAGAC
BsaAI 2050 CACGTG [CT]AC^GT[GA]
3064 CACGTG
BsaWI 2310 TCCGGA [AT]^CCGG[AT]
3284 TCCGGA
BseAI 2310 TCCGGA T^CCGGA
3284 TCCGGA
BspEI 2310 TCCGGA T^CCGGA
3284 TCCGGA
BspHI 1568 TCATGA T^CATGA
2759 TCATGA
BssSI 2170 CACGAG CACGAG(-5/-1)
4252 CACGAG
BstAPI 1307 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3387 GCACGGGCTGC
BstEII 163 GGTCACC G^GT[ACGT]ACC
2584 GGTGACC
BstPI 163 GGTCACC G^GT[ACGT]ACC
2584 GGTGACC
BstYI 1244 AGATCC [GA]^GATC[CT]
2491 GGATCC
EcoNI 239 CCTCCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
903 CCTGCTGGAGG
EcoO65I 163 GGTCACC G^GT[ACGT]ACC
2584 GGTGACC
MflI 1244 AGATCC [GA]^GATC[CT]
2491 GGATCC
MluNI 4208 TGGCCA TGG^CCA
4220 TGGCCA
MroI 2310 TCCGGA T^CCGGA
3284 TCCGGA
MscI 4208 TGGCCA TGG^CCA
4220 TGGCCA
NcoI 1520 CCATGG C^CATGG
3894 CCATGG
PaeR7I 3480 CTCGAG C^TCGAG
3859 CTCGAG
PflMI 900 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3669 CCACACGGTGG
PmaCI 2050 CACGTG CAC^GTG
3064 CACGTG
PmlI 2050 CACGTG CAC^GTG
3064 CACGTG
PpuMI 1645 GGGACCC [GA]G^G[AT]CC[CT]
3050 GGGACCT
PshAI 3998 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4572 GACCCCAGTC
RcaI 1568 TCATGA T^CATGA
2759 TCATGA
Van91I 900 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3669 CCACACGGTGG
XhoI 3480 CTCGAG C^TCGAG
3859 CTCGAG
XhoII 1244 AGATCC [GA]^GATC[CT]
2491 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI Aor51HI ApaLI
AspHI AvaI AvaII AviII BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstXI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmaCI XmaI