Restriction map for
pF1KA1012
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# NO CUT ENZYMES ( 83 enzymes) #
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AatII Acc65I AclI AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AspI AvaI AviII
BamHI BbrPI BcgI BglI BlpI
Bpu1102I BsiEI BsiWI BsmI BsoBI
BspLU11I BspMI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
FseI FspI KpnI KspI MluI
NaeI NgoMIV NheI NotI NruI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI Sse8387I
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 3004 GTAGAC GT^[AC][GT]AC
AccII 117 CGCG CG^CG
AccIII 254 TCCGGA T^CCGGA
AcyI 1823 GGCGCC G[GA]^CG[CT]C
AflII 127 CTTAAG C^TTAAG
Alw44I 1613 GTGCAC G^TGCAC
ApaLI 1613 GTGCAC G^TGCAC
BalI 2037 TGGCCA TGG^CCA
BbeI 1823 GGCGCC GGCGC^C
BfrI 127 CTTAAG C^TTAAG
BglII 955 AGATCT A^GATCT
BsaHI 1823 GGCGCC G[GA]^CG[CT]C
BsaI 1787 GGTCTC GGTCTC(1/5)
BsaWI 254 TCCGGA [AT]^CCGG[AT]
BseAI 254 TCCGGA T^CCGGA
BseRI 3290 GAGGAG GAGGAG(10/8)
BsmBI 97 CGTCTC CGTCTC(1/5)
BspDI 1706 ATCGAT AT^CGAT
BspEI 254 TCCGGA T^CCGGA
BspHI 414 TCATGA T^CATGA
BsrBI 85 GAGCGG CCGCTC(-3/-3)
BstBI 1607 TTCGAA TT^CGAA
BstUI 117 CGCG CG^CG
BstXI 724 CCAGATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1706 ATCGAT AT^CGAT
DraIII 3361 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 292 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1679 ATGCAT ATGCA^T
FauI 140 CCCGC CCCGC(4/6)
HaeII 1823 GGCGCC [GA]GCGC^[CT]
HgaI 454 GCGTC GACGC(5/10)
Hin1I 1823 GGCGCC G[GA]^CG[CT]C
HindIII 259 AAGCTT A^AGCTT
HpaI 2161 GTTAAC GTT^AAC
KasI 1823 GGCGCC G^GCGCC
MfeI 4017 CAATTG C^AATTG
MluNI 2037 TGGCCA TGG^CCA
MroI 254 TCCGGA T^CCGGA
MscI 2037 TGGCCA TGG^CCA
MunI 4017 CAATTG C^AATTG
MvnI 117 CGCG CG^CG
NarI 1823 GGCGCC GG^CGCC
NsiI 1679 ATGCAT ATGCA^T
NspV 1607 TTCGAA TT^CGAA
PacI 2437 TTAATTAA TTAAT^TAA
PpuMI 2643 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 414 TCATGA T^CATGA
SacI 887 GAGCTC GAGCT^C
SapI 2569 GCTCTTC GCTCTTC(1/4)
SfoI 1823 GGCGCC GGC^GCC
SfuI 1607 TTCGAA TT^CGAA
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# TWO CUT ENZYMES ( 30 enzymes) #
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AvrII 1429 CCTAGG C^CTAGG
4213 CCTAGG
BanI 1381 GGTGCC G^G[CT][GA]CC
1823 GGCGCC
BanII 887 GAGCTC G[GA]GC[CT]^C
2541 GGGCTC
BbsI 3748 GAAGAC GAAGAC(2/6)
3938 GTCTTC
BciVI 1455 GGATAC GTATCC(6/5)
1239 GTATCC
BlnI 1429 CCTAGG C^CTAGG
4213 CCTAGG
BpmI 745 CTCCAG CTGGAG(16/14)
245 CTGGAG
BpuAI 3748 GAAGAC GAAGAC(2/6)
3938 GTCTTC
BsaAI 187 CACGTA [CT]AC^GT[GA]
3107 TACGTG
BsaBI 1597 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4287 GATCATCATC
Bsp1407I 12 TGTACA T^GTACA
2974 TGTACA
BsrGI 12 TGTACA T^GTACA
2974 TGTACA
BstAPI 1375 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1399 GCAGTGTCTGC
CfoI 64 GCGC GCG^C
1824 GCGC
DraI 1536 TTTAAA TTT^AAA
3234 TTTAAA
DraII 1073 GGGGCCT [GA]G^G[ACGT]CC[CT]
2643 AGGTCCT
DrdI 40 GACTCCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2313 GACTGATTTGTC
EaeI 2037 TGGCCA [CT]^GGCC[GA]
3503 CGGCCA
EarI 2570 CTCTTC CTCTTC(1/4)
2668 GAAGAG
EcoO109I 1073 GGGGCCT [GA]G^G[ACGT]CC[CT]
2643 AGGTCCT
EcoRV 1480 GATATC GAT^ATC
3538 GATATC
HhaI 64 GCGC GCG^C
1824 GCGC
HinP1I 64 GCGC G^CGC
1824 GCGC
Ksp632I 2570 CTCTTC CTCTTC(1/4)
2668 GAAGAG
MamI 1597 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4287 GATCATCATC
PflMI 2053 CCAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2830 CCACTTACTGG
SpeI 2385 ACTAGT A^CTAGT
3334 ACTAGT
SphI 1734 GCATGC GCATG^C
4174 GCATGC
SspBI 12 TGTACA T^GTACA
2974 TGTACA
Van91I 2053 CCAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2830 CCACTTACTGG
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BbvCI BbvI BclI
BcnI BfaI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DsaI EcoRI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI