Restriction map for
pF1KA1016
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanII BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrGI Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SgrAI SnaBI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 1790 GTCTAC GT^[AC][GT]AC
AccIII 1706 TCCGGA T^CCGGA
Alw44I 1291 GTGCAC G^TGCAC
AocI 406 CCTGAGG CC^T[ACGT]AGG
ApaLI 1291 GTGCAC G^TGCAC
Asp700I 1750 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 2103 GGACC G^G[AT]CC
BalI 1916 TGGCCA TGG^CCA
BanI 118 GGCGCC G^G[CT][GA]CC
BbeI 118 GGCGCC GGCGC^C
BbrPI 937 CACGTG CAC^GTG
BfaI 688 CTAG C^TAG
BglII 693 AGATCT A^GATCT
BsaAI 937 CACGTG [CT]AC^GT[GA]
BseAI 1706 TCCGGA T^CCGGA
BsiEI 1864 CGGTCG CG[GA][CT]^CG
BsmI 2169 GAATGC GAATGC(1/-1)
BspEI 1706 TCCGGA T^CCGGA
BspMI 167 ACCTGC ACCTGC(4/8)
BsrBI 2094 CCGCTC CCGCTC(-3/-3)
BsrDI 1348 GCAATG GCAATG(2/0)
BsrFI 115 ACCGGC [GA]^CCGG[CT]
BstBI 2030 TTCGAA TT^CGAA
Bsu36I 406 CCTGAGG CC^T[ACGT]AGG
Cfr10I 115 ACCGGC [GA]^CCGG[CT]
DraI 1431 TTTAAA TTT^AAA
DraII 2102 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 406 CCTGAGG CC^T[ACGT]AGG
EcoO109I 2102 GGGACCT [GA]G^G[ACGT]CC[CT]
HgaI 6 GACGC GACGC(5/10)
KasI 118 GGCGCC G^GCGCC
MaeI 688 CTAG C^TAG
MaeII 938 ACGT A^CGT
MluNI 1916 TGGCCA TGG^CCA
MroI 1706 TCCGGA T^CCGGA
MscI 1916 TGGCCA TGG^CCA
NarI 118 GGCGCC GG^CGCC
NcoI 911 CCATGG C^CATGG
NspI 871 GCATGC [GA]CATG^[CT]
NspV 2030 TTCGAA TT^CGAA
PmaCI 937 CACGTG CAC^GTG
PmlI 937 CACGTG CAC^GTG
PpuMI 2102 GGGACCT [GA]G^G[AT]CC[CT]
SapI 1264 GAAGAGC GCTCTTC(1/4)
SfoI 118 GGCGCC GGC^GCC
SfuI 2030 TTCGAA TT^CGAA
SmaI 274 CCCGGG CCC^GGG
SpeI 687 ACTAGT A^CTAGT
SphI 871 GCATGC GCATG^C
XcmI 1904 CCAAACTTAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 274 CCCGGG C^CCGGG
XmaI 274 CCCGGG C^CCGGG
XmnI 1750 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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AccII 193 CGCG CG^CG
195 CGCG
AcyI 6 GACGCC G[GA]^CG[CT]C
118 GGCGCC
AfaI 263 GTAC GT^AC
1972 GTAC
AlwNI 427 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1976 CAGAGGCTG
AspHI 733 GTGCTC G[AT]GC[AT]^C
1291 GTGCAC
AvaI 274 CCCGGG C^[CT]CG[GA]G
2059 CTCGGG
BmyI 733 GTGCTC G[AGT]GC[ACT]^C
1291 GTGCAC
BsaHI 6 GACGCC G[GA]^CG[CT]C
118 GGCGCC
BsaI 1190 GAGACC GGTCTC(1/5)
1216 GGTCTC
BseRI 809 CTCCTC GAGGAG(10/8)
202 GAGGAG
BsiHKAI 733 GTGCTC G[AT]GC[AT]^C
1291 GTGCAC
BsoBI 274 CCCGGG C^[CT]CG[GA]G
2059 CTCGGG
Bsp1286I 733 GTGCTC G[AGT]GC[ACT]^C
1291 GTGCAC
BssHII 192 GCGCGC G^CGCGC
194 GCGCGC
BssSI 736 CTCGTG CACGAG(-5/-1)
1092 CTCGTG
BstUI 193 CGCG CG^CG
195 CGCG
BstYI 693 AGATCT [GA]^GATC[CT]
1389 AGATCC
Cfr13I 39 GGCCC G^G[ACGT]CC
2103 GGACC
EaeI 209 CGGCCA [CT]^GGCC[GA]
1916 TGGCCA
EcoT14I 701 CCTTGG C^C[AT][AT]GG
911 CCATGG
HaeII 118 GGCGCC [GA]GCGC^[CT]
235 AGCGCC
Hin1I 6 GACGCC G[GA]^CG[CT]C
118 GGCGCC
HincII 1846 GTCAAC GT[CT]^[GA]AC
2041 GTTGAC
HindII 1846 GTCAAC GT[CT]^[GA]AC
2041 GTTGAC
HphI 1162 GGTGA GGTGA(8/7)
1891 TCACC
MflI 693 AGATCT [GA]^GATC[CT]
1389 AGATCC
MslI 1767 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2164 CACTGGAATG
MvnI 193 CGCG CG^CG
195 CGCG
PflMI 906 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
933 CCAGCACGTGG
RsaI 263 GTAC GT^AC
1972 GTAC
Sau96I 39 GGCCC G^G[ACGT]CC
2103 GGACC
SfaNI 429 GATGC GCATC(5/9)
1877 GCATC
StyI 701 CCTTGG C^C[AT][AT]GG
911 CCATGG
Van91I 906 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
933 CCAGCACGTGG
XhoII 693 AGATCT [GA]^GATC[CT]
1389 AGATCC
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbsI BbvI BcnI BpmI BpuAI
BsaJI BsaWI BsgI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cac8I CfoI DdeI DpnII
DsaI EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
Sau3AI ScrFI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I