Restriction map for
pF1KA1031
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AocI Aor51HI ApoI AscI
AseI AsnI Asp718I AspI AviII
AvrII BbeI BbrPI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsiWI
BsmBI BsmI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccIII 1373 TCCGGA T^CCGGA
Alw44I 1774 GTGCAC G^TGCAC
ApaLI 1774 GTGCAC G^TGCAC
Asp700I 2604 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1403 GGATCC G^GATCC
BbvCI 121 GCTGAGG CCTCAGC(-5/-2)
BcgI 198 CGACTACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 344 GTATCC GTATCC(6/5)
BglI 833 GCCTGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1664 AGATCT A^GATCT
BsaAI 631 TACGTG [CT]AC^GT[GA]
BsaWI 1373 TCCGGA [AT]^CCGG[AT]
BseAI 1373 TCCGGA T^CCGGA
BspEI 1373 TCCGGA T^CCGGA
BsrDI 128 GCAATG GCAATG(2/0)
BstAPI 2693 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 769 GACGGGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1573 CTCTTC CTCTTC(1/4)
Ksp632I 1573 CTCTTC CTCTTC(1/4)
MroI 1373 TCCGGA T^CCGGA
MslI 2511 CATCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1367 GCCGGC GCC^GGC
NgoMIV 1367 GCCGGC G^CCGGC
PaeR7I 641 CTCGAG C^TCGAG
PshAI 1156 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1572 GCTCTTC GCTCTTC(1/4)
SexAI 215 ACCAGGT A^CC[AT]GGT
TfiI 111 GAATC G^A[AT]TC
Tsp509I 527 AATT ^AATT
XcmI 2021 CCAGCAGCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 641 CTCGAG C^TCGAG
XmnI 2604 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
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AccI 54 GTCGAC GT^[AC][GT]AC
1098 GTCGAC
AcyI 389 GACGCC G[GA]^CG[CT]C
2626 GACGCC
AhdI 808 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1711 GACTCTCCGTC
ApaI 1026 GGGCCC GGGCC^C
2053 GGGCCC
AspEI 808 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1711 GACTCTCCGTC
BalI 2091 TGGCCA TGG^CCA
2789 TGGCCA
BbsI 1960 GAAGAC GAAGAC(2/6)
2608 GTCTTC
BfaI 854 CTAG C^TAG
1169 CTAG
BpuAI 1960 GAAGAC GAAGAC(2/6)
2608 GTCTTC
BsaHI 389 GACGCC G[GA]^CG[CT]C
2626 GACGCC
BsaI 1066 GAGACC GGTCTC(1/5)
1141 GAGACC
BsiEI 1365 CGGCCG CG[GA][CT]^CG
2295 CGGCCG
BspHI 2366 TCATGA T^CATGA
2513 TCATGA
BspLU11I 2354 ACATGT A^CATGT
2408 ACATGT
BssSI 1103 CACGAG CACGAG(-5/-1)
1255 CACGAG
BstXI 983 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1427 CCAGCTTCGTGG
EagI 1365 CGGCCG C^GGCCG
2295 CGGCCG
Eam1105I 808 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1711 GACTCTCCGTC
EclXI 1365 CGGCCG C^GGCCG
2295 CGGCCG
Eco52I 1365 CGGCCG C^GGCCG
2295 CGGCCG
EcoNI 1875 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2440 CCTGGAGAAGG
HaeII 300 GGCGCT [GA]GCGC^[CT]
321 GGCGCT
HgaI 389 GACGC GACGC(5/10)
2626 GACGC
Hin1I 389 GACGCC G[GA]^CG[CT]C
2626 GACGCC
HincII 54 GTCGAC GT[CT]^[GA]AC
1098 GTCGAC
HindII 54 GTCGAC GT[CT]^[GA]AC
1098 GTCGAC
MaeI 854 CTAG C^TAG
1169 CTAG
MluNI 2091 TGGCCA TGG^CCA
2789 TGGCCA
MscI 2091 TGGCCA TGG^CCA
2789 TGGCCA
MseI 1466 TTAA T^TAA
1595 TTAA
PvuII 868 CAGCTG CAG^CTG
1887 CAGCTG
RcaI 2366 TCATGA T^CATGA
2513 TCATGA
SalI 54 GTCGAC G^TCGAC
1098 GTCGAC
SphI 1208 GCATGC GCATG^C
2007 GCATGC
Tru9I 1466 TTAA T^TAA
1595 TTAA
Tsp45I 454 GTGAC ^GT[GC]AC
2624 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TseI TspRI TthHB8I
Van91I XhoII XmaCI XmaI