Restriction map for
pF1KA1040
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AspI
AvrII BbeI BbrPI BbvCI BcgI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BsrBI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FauI FseI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SapI SbfI
SfoI SfuI SmaI SnaBI Sse8387I
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1578 GTCGAC GT^[AC][GT]AC
AccII 64 CGCG CG^CG
AocI 4627 CCTAAGG CC^T[ACGT]AGG
ApaI 3601 GGGCCC GGGCC^C
AseI 2626 ATTAAT AT^TAAT
AsnI 2626 ATTAAT AT^TAAT
AspHI 4306 GTGCTC G[AT]GC[AT]^C
AvaI 19 CCCGAG C^[CT]CG[GA]G
AviII 3162 TGCGCA TGC^GCA
BamHI 3105 GGATCC G^GATCC
BclI 2748 TGATCA T^GATCA
BcnI 4433 CCCGG CC^[GC]GG
BglI 2703 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 610 AGATCT A^GATCT
BsaBI 3709 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2439 GAGACC GGTCTC(1/5)
BsiHKAI 4306 GTGCTC G[AT]GC[AT]^C
BsmBI 3340 GAGACG CGTCTC(1/5)
BsoBI 19 CCCGAG C^[CT]CG[GA]G
Bsp1407I 201 TGTACA T^GTACA
BspHI 414 TCATGA T^CATGA
BsrFI 4194 ACCGGC [GA]^CCGG[CT]
BsrGI 201 TGTACA T^GTACA
BssHII 63 GCGCGC G^CGCGC
BstAPI 2670 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 64 CGCG CG^CG
BstXI 1451 CCATGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4627 CCTAAGG CC^T[ACGT]AGG
Cfr10I 4194 ACCGGC [GA]^CCGG[CT]
DsaI 2067 CCATGG C^C[GA][CT]GG
Eco81I 4627 CCTAAGG CC^T[ACGT]AGG
EcoNI 4795 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2748 TGATCA T^GATCA
FspI 3162 TGCGCA TGC^GCA
MamI 3709 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 64 CGCG CG^CG
NciI 4433 CCCGG CC^[GC]GG
NcoI 2067 CCATGG C^CATGG
PpuMI 2187 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 275 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2626 ATTAAT AT^TAAT
RcaI 414 TCATGA T^CATGA
SalI 1578 GTCGAC G^TCGAC
SexAI 3739 ACCTGGT A^CC[AT]GGT
SfiI 2702 GGCCTGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 4193 CACCGGCG C[GA]^CCGG[CT]G
SpeI 5094 ACTAGT A^CTAGT
SspBI 201 TGTACA T^GTACA
XbaI 2328 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflII 364 CTTAAG C^TTAAG
4969 CTTAAG
AvaII 2188 GGACC G^G[AT]CC
4048 GGACC
BanI 7 GGCACC G^G[CT][GA]CC
2158 GGTGCC
BanII 3601 GGGCCC G[GA]GC[CT]^C
4668 GGGCTC
BfrI 364 CTTAAG C^TTAAG
4969 CTTAAG
BseRI 808 CTCCTC GAGGAG(10/8)
4399 CTCCTC
BspMI 774 GCAGGT ACCTGC(4/8)
2857 GCAGGT
DraII 2187 AGGACCC [GA]G^G[ACGT]CC[CT]
3600 AGGGCCC
EcoO109I 2187 AGGACCC [GA]G^G[ACGT]CC[CT]
3600 AGGGCCC
HindIII 2513 AAGCTT A^AGCTT
3504 AAGCTT
MaeII 730 ACGT A^CGT
3343 ACGT
MfeI 143 CAATTG C^AATTG
3155 CAATTG
MunI 143 CAATTG C^AATTG
3155 CAATTG
PflMI 4089 CCAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4126 CCACAGTATGG
SphI 929 GCATGC GCATG^C
4440 GCATGC
Van91I 4089 CCAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4126 CCACAGTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I BalI BbsI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI