Restriction map for
pF1KA1080
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsgI
BsiWI BspDI BspHI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoT22I FseI HaeII
Hin1I HincII HindII HpaI KasI
KpnI MaeI MaeII MamI MfeI
MluI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 426 TCCGGA T^CCGGA
AgeI 632 ACCGGT A^CCGGT
AlwI 210 GATCC GGATC(4/5)
ApaI 60 GGGCCC GGGCC^C
AviII 1074 TGCGCA TGC^GCA
BalI 302 TGGCCA TGG^CCA
BclI 329 TGATCA T^GATCA
BlpI 744 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 744 GCTGAGC GC^T[ACGT]AGC
BseAI 426 TCCGGA T^CCGGA
BsiEI 8 CGACCG CG[GA][CT]^CG
BsmBI 446 GAGACG CGTCTC(1/5)
BsmI 1666 GCATTC GAATGC(1/-1)
Bsp1407I 926 TGTACA T^GTACA
BspEI 426 TCCGGA T^CCGGA
BsrGI 926 TGTACA T^GTACA
BstAPI 735 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 951 GGTCACC G^GT[ACGT]ACC
BstPI 951 GGTCACC G^GT[ACGT]ACC
BstYI 209 AGATCC [GA]^GATC[CT]
CelII 744 GCTGAGC GC^T[ACGT]AGC
DsaI 11 CCGCGG C^C[GA][CT]GG
EaeI 302 TGGCCA [CT]^GGCC[GA]
EcoNI 1144 CCTGTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 951 GGTCACC G^GT[ACGT]ACC
EcoRI 1493 GAATTC G^AATTC
FbaI 329 TGATCA T^GATCA
FspI 1074 TGCGCA TGC^GCA
KspI 11 CCGCGG CCGC^GG
MflI 209 AGATCC [GA]^GATC[CT]
MluNI 302 TGGCCA TGG^CCA
MroI 426 TCCGGA T^CCGGA
MscI 302 TGGCCA TGG^CCA
NaeI 1641 GCCGGC GCC^GGC
NgoMIV 1641 GCCGGC G^CCGGC
PflMI 1213 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 632 ACCGGT A^CCGGT
PvuII 1688 CAGCTG CAG^CTG
SacI 543 GAGCTC GAGCT^C
SacII 11 CCGCGG CCGC^GG
SbfI 792 CCTGCAGG CCTGCA^GG
SexAI 1332 ACCAGGT A^CC[AT]GGT
SmaI 57 CCCGGG CCC^GGG
SmlI 1415 CTCAAG C^T[CT][GA]AG
Sse8387I 792 CCTGCAGG CCTGCA^GG
SspBI 926 TGTACA T^GTACA
TaqI 444 TCGA T^CGA
TthHB8I 444 TCGA T^CGA
Van91I 1213 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 209 AGATCC [GA]^GATC[CT]
XmaCI 57 CCCGGG C^CCGGG
XmaI 57 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 12 CGCG CG^CG
879 CGCG
Asp700I 1482 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1803 GAAAGACTTC
AspHI 543 GAGCTC G[AT]GC[AT]^C
1566 GAGCAC
AvaI 57 CCCGGG C^[CT]CG[GA]G
610 CCCGAG
BanI 1092 GGTGCC G^G[CT][GA]CC
1529 GGCACC
BbsI 430 GAAGAC GAAGAC(2/6)
1032 GAAGAC
BpuAI 430 GAAGAC GAAGAC(2/6)
1032 GAAGAC
BsiHKAI 543 GAGCTC G[AT]GC[AT]^C
1566 GAGCAC
BsoBI 57 CCCGGG C^[CT]CG[GA]G
610 CCCGAG
BsrFI 632 ACCGGT [GA]^CCGG[CT]
1641 GCCGGC
BstF5I 990 CATCC GGATG(2/0)
1646 CATCC
BstUI 12 CGCG CG^CG
879 CGCG
BstXI 526 CCATCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
974 CCAGCTCATTGG
Cfr10I 632 ACCGGT [GA]^CCGG[CT]
1641 GCCGGC
DpnII 210 GATC ^GATC
330 GATC
DrdI 988 GACATCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1816 GACCTGGCTGTC
EarI 403 CTCTTC CTCTTC(1/4)
386 GAAGAG
EcoRV 1591 GATATC GAT^ATC
1695 GATATC
FauI 774 CCCGC CCCGC(4/6)
31 GCGGG
FokI 990 CATCC GGATG(9/13)
1646 CATCC
HgaI 448 GACGC GACGC(5/10)
70 GCGTC
HindIII 577 AAGCTT A^AGCTT
1657 AAGCTT
Ksp632I 403 CTCTTC CTCTTC(1/4)
386 GAAGAG
MboI 210 GATC ^GATC
330 GATC
MspA1I 11 CCGCGG C[AC]G^C[GT]G
1688 CAGCTG
MvnI 12 CGCG CG^CG
879 CGCG
NdeII 210 GATC ^GATC
330 GATC
Sau3AI 210 GATC ^GATC
330 GATC
XcmI 523 CCACCATCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1351 CCAGGTTGGTCCTGG
XmnI 1482 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1803 GAAAGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboII MnlI MseI
MspI MvaI MwoI NciI NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI