Restriction map for
pF1KA1081
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglI
BlnI BsaAI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BspMI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV FbaI FseI FspI HaeII
HapII HincII HindII HpaI HpaII
KasI KpnI KspI MluI MroI
MspI NaeI NarI NciI NdeI
NgoMIV NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 287 GACGTC GACGT^C
AccII 2369 CGCG CG^CG
AflIII 1607 ACATGT A^C[GA][CT]GT
Alw44I 2348 GTGCAC G^TGCAC
AocI 1976 CCTCAGG CC^T[ACGT]AGG
ApaLI 2348 GTGCAC G^TGCAC
AspHI 2348 GTGCAC G[AT]GC[AT]^C
AvaI 911 CTCGGG C^[CT]CG[GA]G
BamHI 710 GGATCC G^GATCC
BglII 1872 AGATCT A^GATCT
BlpI 814 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 814 GCTGAGC GC^T[ACGT]AGC
BsaBI 678 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1196 GAGACC GGTCTC(1/5)
BsiHKAI 2348 GTGCAC G[AT]GC[AT]^C
BsmI 1107 GAATGC GAATGC(1/-1)
BsoBI 911 CTCGGG C^[CT]CG[GA]G
BspLU11I 1607 ACATGT A^CATGT
BstBI 851 TTCGAA TT^CGAA
BstEII 91 GGTCACC G^GT[ACGT]ACC
BstPI 91 GGTCACC G^GT[ACGT]ACC
BstUI 2369 CGCG CG^CG
BstXI 45 CCAGAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1976 CCTCAGG CC^T[ACGT]AGG
CelII 814 GCTGAGC GC^T[ACGT]AGC
CfoI 2368 GCGC GCG^C
DraI 183 TTTAAA TTT^AAA
DsaI 2567 CCATGG C^C[GA][CT]GG
Eco81I 1976 CCTCAGG CC^T[ACGT]AGG
EcoO65I 91 GGTCACC G^GT[ACGT]ACC
EcoT22I 1090 ATGCAT ATGCA^T
HhaI 2368 GCGC GCG^C
HinP1I 2368 GCGC G^CGC
MamI 678 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2369 CGCG CG^CG
NcoI 2567 CCATGG C^CATGG
NsiI 1090 ATGCAT ATGCA^T
NspV 851 TTCGAA TT^CGAA
PpuMI 719 GGGACCT [GA]G^G[AT]CC[CT]
ScaI 1243 AGTACT AGT^ACT
SexAI 2149 ACCAGGT A^CC[AT]GGT
SfuI 851 TTCGAA TT^CGAA
SphI 1956 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 287 GACGTC G[GA]^CG[CT]C
2743 GACGCC
AflII 2002 CTTAAG C^TTAAG
2842 CTTAAG
Asp700I 1657 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2713 GAAGCTCTTC
AvaII 720 GGACC G^G[AT]CC
2528 GGACC
BalI 1320 TGGCCA TGG^CCA
2564 TGGCCA
BbsI 982 GAAGAC GAAGAC(2/6)
2784 GAAGAC
BfrI 2002 CTTAAG C^TTAAG
2842 CTTAAG
BpuAI 982 GAAGAC GAAGAC(2/6)
2784 GAAGAC
BsaHI 287 GACGTC G[GA]^CG[CT]C
2743 GACGCC
Bsp1407I 651 TGTACA T^GTACA
2475 TGTACA
BsrGI 651 TGTACA T^GTACA
2475 TGTACA
DraII 719 GGGACCT [GA]G^G[ACGT]CC[CT]
1775 AGGCCCT
EcoNI 426 CCTTCGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1200 CCTGGAAGAGG
EcoO109I 719 GGGACCT [GA]G^G[ACGT]CC[CT]
1775 AGGCCCT
EcoT14I 968 CCAAGG C^C[AT][AT]GG
2567 CCATGG
HgaI 2743 GACGC GACGC(5/10)
2853 GACGC
Hin1I 287 GACGTC G[GA]^CG[CT]C
2743 GACGCC
HindIII 611 AAGCTT A^AGCTT
931 AAGCTT
MfeI 1799 CAATTG C^AATTG
2062 CAATTG
MluNI 1320 TGGCCA TGG^CCA
2564 TGGCCA
MscI 1320 TGGCCA TGG^CCA
2564 TGGCCA
MspA1I 469 CAGCTG C[AC]G^C[GT]G
730 CAGCTG
MunI 1799 CAATTG C^AATTG
2062 CAATTG
NheI 340 GCTAGC G^CTAGC
2505 GCTAGC
PvuII 469 CAGCTG CAG^CTG
730 CAGCTG
SapI 2446 GAAGAGC GCTCTTC(1/4)
2716 GCTCTTC
SspBI 651 TGTACA T^GTACA
2475 TGTACA
StyI 968 CCAAGG C^C[AT][AT]GG
2567 CCATGG
XmnI 1657 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2713 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII