Restriction map for
pF1KA1090
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII Acc65I AccI AccIII AflII
AgeI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BbeI BbrPI BclI BfaI
BfrI BlnI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco52I
FbaI FseI FspI KasI KpnI
MaeI MluI MluNI MroI MscI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SacI SalI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AclI 661 AACGTT AA^CGTT
AflIII 3857 ACGTGT A^C[GA][CT]GT
AhdI 3952 GACGGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 773 GTGCAC G^TGCAC
AlwNI 1280 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2183 AGCGCT AGC^GCT
ApaI 956 GGGCCC GGGCC^C
ApaLI 773 GTGCAC G^TGCAC
AspEI 3952 GACGGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 1592 GGATAC GTATCC(6/5)
BlpI 1417 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1417 GCTGAGC GC^T[ACGT]AGC
BsaAI 787 CACGTA [CT]AC^GT[GA]
BsaBI 3064 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1898 GAGACG CGTCTC(1/5)
BsmI 1181 GCATTC GAATGC(1/-1)
BsrBI 1015 GAGCGG CCGCTC(-3/-3)
BstEII 694 GGTTACC G^GT[ACGT]ACC
BstPI 694 GGTTACC G^GT[ACGT]ACC
CelII 1417 GCTGAGC GC^T[ACGT]AGC
Eam1105I 3952 GACGGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2183 AGCGCT AGC^GCT
EcoNI 168 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 694 GGTTACC G^GT[ACGT]ACC
EcoRI 2749 GAATTC G^AATTC
EcoRV 2644 GATATC GAT^ATC
EcoT22I 1775 ATGCAT ATGCA^T
HindIII 2917 AAGCTT A^AGCTT
HpaI 3265 GTTAAC GTT^AAC
KspI 3140 CCGCGG CCGC^GG
MamI 3064 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3588 CAATTG C^AATTG
MunI 3588 CAATTG C^AATTG
NaeI 2682 GCCGGC GCC^GGC
NcoI 1721 CCATGG C^CATGG
NgoMIV 2682 GCCGGC G^CCGGC
NsiI 1775 ATGCAT ATGCA^T
PshAI 1329 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 661 AACGTT AA^CGTT
SacII 3140 CCGCGG CCGC^GG
ScaI 1261 AGTACT AGT^ACT
SspI 2719 AATATT AAT^ATT
StuI 3782 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 205 GACGCC G[GA]^CG[CT]C
805 GACGCC
AlwI 727 GATCC GGATC(4/5)
2858 GGATC
AocI 1127 CCTCAGG CC^T[ACGT]AGG
2132 CCTCAGG
BanI 69 GGCACC G^G[CT][GA]CC
900 GGCACC
BanII 956 GGGCCC G[GA]GC[CT]^C
1903 GAGCCC
BbvCI 141 CCTCAGC CCTCAGC(-5/-2)
1621 GCTGAGG
BcgI 1659 GCACAGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1729 GCAGCCTCCTCG
BsaHI 205 GACGCC G[GA]^CG[CT]C
805 GACGCC
BspMI 2116 ACCTGC ACCTGC(4/8)
3129 GCAGGT
BsrFI 2682 GCCGGC [GA]^CCGG[CT]
3572 GCCGGT
Bsu36I 1127 CCTCAGG CC^T[ACGT]AGG
2132 CCTCAGG
Cfr10I 2682 GCCGGC [GA]^CCGG[CT]
3572 GCCGGT
Eco81I 1127 CCTCAGG CC^T[ACGT]AGG
2132 CCTCAGG
HaeII 2183 AGCGCT [GA]GCGC^[CT]
3176 AGCGCC
Hin1I 205 GACGCC G[GA]^CG[CT]C
805 GACGCC
HincII 2485 GTCAAC GT[CT]^[GA]AC
3265 GTTAAC
HindII 2485 GTCAAC GT[CT]^[GA]AC
3265 GTTAAC
PflMI 622 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1374 CCAGTTTTTGG
PpuMI 1598 AGGACCC [GA]G^G[AT]CC[CT]
2049 AGGACCC
SapI 834 GAAGAGC GCTCTTC(1/4)
1450 GAAGAGC
SbfI 168 CCTGCAGG CCTGCA^GG
3508 CCTGCAGG
SmaI 251 CCCGGG CCC^GGG
1893 CCCGGG
Sse8387I 168 CCTGCAGG CCTGCA^GG
3508 CCTGCAGG
Van91I 622 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1374 CCAGTTTTTGG
XcmI 811 CCAGAAAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1511 CCAAGTTTGCCCTGG
XmaCI 251 CCCGGG C^CCGGG
1893 CCCGGG
XmaI 251 CCCGGG C^CCGGG
1893 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI AvaI AvaII BbsI
BbvI BcnI BglI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII