Restriction map for
pF1KA1104
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII Acc65I AccIII AclI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII BamHI
BbeI BcgI BclI BglI BsaBI
BseAI Bsp1407I BspEI BsrGI BssHII
Bst1107I BstBI BstZ17I CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SwaI Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 517 CGCG CG^CG
AcyI 579 GACGCC G[GA]^CG[CT]C
AflII 748 CTTAAG C^TTAAG
AgeI 383 ACCGGT A^CCGGT
AocI 2283 CCTGAGG CC^T[ACGT]AGG
ApaI 1066 GGGCCC GGGCC^C
Asp700I 405 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1723 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 916 CCTAGG C^CTAGG
BbrPI 1951 CACGTG CAC^GTG
BciVI 435 GTATCC GTATCC(6/5)
BfrI 748 CTTAAG C^TTAAG
BglII 1607 AGATCT A^GATCT
BlnI 916 CCTAGG C^CTAGG
BsaHI 579 GACGCC G[GA]^CG[CT]C
BsiWI 800 CGTACG C^GTACG
BsmBI 2788 GAGACG CGTCTC(1/5)
BspDI 1506 ATCGAT AT^CGAT
BspHI 401 TCATGA T^CATGA
BspLU11I 934 ACATGT A^CATGT
BsrBI 48 GAGCGG CCGCTC(-3/-3)
BsrDI 782 GCAATG GCAATG(2/0)
BsrFI 383 ACCGGT [GA]^CCGG[CT]
BstAPI 1989 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 709 GGTGACC G^GT[ACGT]ACC
BstPI 709 GGTGACC G^GT[ACGT]ACC
BstUI 517 CGCG CG^CG
Bsu36I 2283 CCTGAGG CC^T[ACGT]AGG
Cfr10I 383 ACCGGT [GA]^CCGG[CT]
ClaI 1506 ATCGAT AT^CGAT
DraI 592 TTTAAA TTT^AAA
DrdI 2790 GACGCTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2283 CCTGAGG CC^T[ACGT]AGG
EcoO65I 709 GGTGACC G^GT[ACGT]ACC
EcoT22I 2604 ATGCAT ATGCA^T
HaeII 5 GGCGCT [GA]GCGC^[CT]
Hin1I 579 GACGCC G[GA]^CG[CT]C
MvnI 517 CGCG CG^CG
NsiI 2604 ATGCAT ATGCA^T
PflFI 1723 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 383 ACCGGT A^CCGGT
PmaCI 1951 CACGTG CAC^GTG
PmlI 1951 CACGTG CAC^GTG
PvuII 1736 CAGCTG CAG^CTG
RcaI 401 TCATGA T^CATGA
SalI 2815 GTCGAC G^TCGAC
SapI 3036 GAAGAGC GCTCTTC(1/4)
SexAI 155 ACCAGGT A^CC[AT]GGT
SspI 2058 AATATT AAT^ATT
Tth111I 1723 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1623 TCTAGA T^CTAGA
XmnI 405 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 1317 GTCTAC GT^[AC][GT]AC
2815 GTCGAC
AflIII 686 ACACGT A^C[GA][CT]GT
934 ACATGT
BalI 236 TGGCCA TGG^CCA
3053 TGGCCA
BanI 888 GGTGCC G^G[CT][GA]CC
2153 GGCACC
BlpI 45 GCTGAGC GC^T[ACGT]AGC
1906 GCTCAGC
Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC
1906 GCTCAGC
BsgI 268 GTGCAG GTGCAG(16/14)
354 GTGCAG
BsmI 2346 GAATGC GAATGC(1/-1)
2602 GAATGC
BspMI 2027 ACCTGC ACCTGC(4/8)
2204 ACCTGC
CelII 45 GCTGAGC GC^T[ACGT]AGC
1906 GCTCAGC
EcoRI 922 GAATTC G^AATTC
2138 GAATTC
EcoRV 1744 GATATC GAT^ATC
2269 GATATC
HincII 1637 GTCAAC GT[CT]^[GA]AC
2815 GTCGAC
HindII 1637 GTCAAC GT[CT]^[GA]AC
2815 GTCGAC
MluNI 236 TGGCCA TGG^CCA
3053 TGGCCA
MscI 236 TGGCCA TGG^CCA
3053 TGGCCA
MspA1I 1736 CAGCTG C[AC]G^C[GT]G
2220 CAGCGG
NspI 57 ACATGC [GA]CATG^[CT]
934 ACATGT
SacI 1387 GAGCTC GAGCT^C
2125 GAGCTC
SbfI 1431 CCTGCAGG CCTGCA^GG
2205 CCTGCAGG
ScaI 295 AGTACT AGT^ACT
1757 AGTACT
Sse8387I 1431 CCTGCAGG CCTGCA^GG
2205 CCTGCAGG
StuI 1157 AGGCCT AGG^CCT
2431 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BssSI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII