Restriction map for
pF1KA1128
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII AccII AclI AcyI AflII
AgeI AhdI Aor51HI ApaI AscI
AspEI AspI AviII AvrII BalI
BamHI BanII BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaAI BsaHI BsaI BsgI
BsiEI BsiWI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CfoI Cfr10I
CpoI DraII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRV FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KspI
MfeI MluI MluNI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 2793 GGTACC G^GTACC
AccI 2689 GTAGAC GT^[AC][GT]AC
AccIII 2457 TCCGGA T^CCGGA
AseI 637 ATTAAT AT^TAAT
AsnI 637 ATTAAT AT^TAAT
Asp718I 2793 GGTACC G^GTACC
AvaI 2674 CCCGAG C^[CT]CG[GA]G
BanI 2793 GGTACC G^G[CT][GA]CC
BbvCI 1818 CCTCAGC CCTCAGC(-5/-2)
BcnI 821 CCCGG CC^[GC]GG
BlpI 605 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 605 GCTTAGC GC^T[ACGT]AGC
BsaWI 2457 TCCGGA [AT]^CCGG[AT]
BseAI 2457 TCCGGA T^CCGGA
BsoBI 2674 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1429 TGTACA T^GTACA
BspDI 3107 ATCGAT AT^CGAT
BspEI 2457 TCCGGA T^CCGGA
BspMI 1026 ACCTGC ACCTGC(4/8)
BsrGI 1429 TGTACA T^GTACA
BstXI 1572 CCATCCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 605 GCTTAGC GC^T[ACGT]AGC
ClaI 3107 ATCGAT AT^CGAT
DraI 2766 TTTAAA TTT^AAA
DraIII 227 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1845 CCATGG C^C[GA][CT]GG
EcoT22I 2437 ATGCAT ATGCA^T
FauI 2411 CCCGC CCCGC(4/6)
KpnI 2793 GGTACC GGTAC^C
MroI 2457 TCCGGA T^CCGGA
MspA1I 542 CAGCTG C[AC]G^C[GT]G
NciI 821 CCCGG CC^[GC]GG
NcoI 1845 CCATGG C^CATGG
NsiI 2437 ATGCAT ATGCA^T
PshBI 637 ATTAAT AT^TAAT
PvuII 542 CAGCTG CAG^CTG
SphI 551 GCATGC GCATG^C
SspBI 1429 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 24 enzymes) #
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Alw44I 224 GTGCAC G^TGCAC
2450 GTGCAC
AlwNI 2804 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2920 CAGAATCTG
ApaLI 224 GTGCAC G^TGCAC
2450 GTGCAC
AspHI 224 GTGCAC G[AT]GC[AT]^C
2450 GTGCAC
AvaII 433 GGACC G^G[AT]CC
1089 GGACC
BmyI 224 GTGCAC G[AGT]GC[ACT]^C
2450 GTGCAC
BsaBI 1651 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1669 GATGTGGATC
BseRI 2324 CTCCTC GAGGAG(10/8)
406 GAGGAG
BsiHKAI 224 GTGCAC G[AT]GC[AT]^C
2450 GTGCAC
BsmBI 2050 CGTCTC CGTCTC(1/5)
2313 GAGACG
BsmFI 97 GGGAC GGGAC(10/14)
994 GGGAC
BsmI 2419 GAATGC GAATGC(1/-1)
3081 GCATTC
Bsp1286I 224 GTGCAC G[AGT]GC[ACT]^C
2450 GTGCAC
BstAPI 81 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2414 GCAGTGAATGC
EcoNI 298 CCTACTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1759 CCTCAGGAAGG
EcoRI 687 GAATTC G^AATTC
2318 GAATTC
HapII 822 CCGG C^CGG
2458 CCGG
HpaII 822 CCGG C^CGG
2458 CCGG
MamI 1651 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1669 GATGTGGATC
MspI 822 CCGG C^CGG
2458 CCGG
SapI 238 GAAGAGC GCTCTTC(1/4)
2509 GAAGAGC
ScaI 834 AGTACT AGT^ACT
2331 AGTACT
StuI 559 AGGCCT AGG^CCT
874 AGGCCT
XcmI 1783 CCACCCCAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1788 CCAGAGGTGGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI Asp700I BbsI
BbvI BfaI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BspLU11I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
EarI Eco81I EcoRII EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI