Restriction map for
pF1KA1140
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I AspI
AvrII BamHI BbrPI BcgI BciVI
BfrI BlnI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BsrBI
BsrDI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FseI HindIII HpaI KspI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SspI SwaI Tru9I Tth111I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 893 GGTACC G^GTACC
AccII 1005 CGCG CG^CG
AcsI 880 GAATTT [GA]^AATT[CT]
AflIII 37 ACATGT A^C[GA][CT]GT
AhdI 824 GACAGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 257 GGATC GGATC(4/5)
ApaI 1746 GGGCCC GGGCC^C
ApoI 880 GAATTT [GA]^AATT[CT]
Asp718I 893 GGTACC G^GTACC
AspEI 824 GACAGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 713 CTCGAG C^[CT]CG[GA]G
AviII 1979 TGCGCA TGC^GCA
BalI 1415 TGGCCA TGG^CCA
BbeI 1224 GGCGCC GGCGC^C
BbsI 288 GAAGAC GAAGAC(2/6)
BglI 601 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 288 GAAGAC GAAGAC(2/6)
BsaAI 2073 TACGTG [CT]AC^GT[GA]
BsaBI 1062 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 517 CTGCAC GTGCAG(16/14)
BsmI 774 GAATGC GAATGC(1/-1)
BsoBI 713 CTCGAG C^[CT]CG[GA]G
BspLU11I 37 ACATGT A^CATGT
BsrFI 1898 GCCGGC [GA]^CCGG[CT]
BstUI 1005 CGCG CG^CG
Cfr10I 1898 GCCGGC [GA]^CCGG[CT]
DrdI 1234 GACCCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 824 GACAGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1987 ATGCAT ATGCA^T
FspI 1979 TGCGCA TGC^GCA
KasI 1224 GGCGCC G^GCGCC
KpnI 893 GGTACC GGTAC^C
MamI 1062 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1415 TGGCCA TGG^CCA
MscI 1415 TGGCCA TGG^CCA
MvnI 1005 CGCG CG^CG
NaeI 1898 GCCGGC GCC^GGC
NarI 1224 GGCGCC GG^CGCC
NgoMIV 1898 GCCGGC G^CCGGC
NsiI 1987 ATGCAT ATGCA^T
PaeR7I 713 CTCGAG C^TCGAG
PflMI 1350 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1365 GCTCTTC GCTCTTC(1/4)
ScaI 1881 AGTACT AGT^ACT
SexAI 896 ACCAGGT A^CC[AT]GGT
SfoI 1224 GGCGCC GGC^GCC
TfiI 700 GAATC G^A[AT]TC
Van91I 1350 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 713 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 1150 GACGCC G[GA]^CG[CT]C
1224 GGCGCC
BbvCI 1521 GCTGAGG CCTCAGC(-5/-2)
1906 GCTGAGG
BclI 230 TGATCA T^GATCA
1067 TGATCA
BfaI 1036 CTAG C^TAG
1567 CTAG
BglII 1283 AGATCT A^GATCT
1778 AGATCT
BsaHI 1150 GACGCC G[GA]^CG[CT]C
1224 GGCGCC
BsaI 457 GAGACC GGTCTC(1/5)
1123 GGTCTC
BsaWI 755 ACCGGA [AT]^CCGG[AT]
1184 ACCGGA
Bsp1407I 1541 TGTACA T^GTACA
1943 TGTACA
BsrGI 1541 TGTACA T^GTACA
1943 TGTACA
BssSI 2080 CACGAG CACGAG(-5/-1)
1501 CTCGTG
BstXI 494 CCAAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1289 CCAGTGCCATGG
BstYI 1283 AGATCT [GA]^GATC[CT]
1778 AGATCT
FbaI 230 TGATCA T^GATCA
1067 TGATCA
HgaI 1150 GACGC GACGC(5/10)
1200 GACGC
Hin1I 1150 GACGCC G[GA]^CG[CT]C
1224 GGCGCC
HincII 1408 GTCAAC GT[CT]^[GA]AC
2140 GTTGAC
HindII 1408 GTCAAC GT[CT]^[GA]AC
2140 GTTGAC
MaeI 1036 CTAG C^TAG
1567 CTAG
MflI 1283 AGATCT [GA]^GATC[CT]
1778 AGATCT
PleI 1654 GACTC GAGTC(4/5)
2010 GAGTC
SspBI 1541 TGTACA T^GTACA
1943 TGTACA
StuI 159 AGGCCT AGG^CCT
1563 AGGCCT
XcmI 1103 CCAAGGGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1457 CCAAGGTGAAGCTGG
XhoII 1283 AGATCT [GA]^GATC[CT]
1778 AGATCT
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanI BanII BbvI BcnI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SmlI SphI Sse8387I StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I