Restriction map for
pF1KA1154
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BanII BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FbaI FseI FspI
HpaI KpnI MamI MfeI MluI
MslI MunI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SbfI ScaI SexAI SfiI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 221 TCCGGA T^CCGGA
AcyI 92 GGCGCC G[GA]^CG[CT]C
AflIII 377 ACGTGT A^C[GA][CT]GT
Alw44I 414 GTGCAC G^TGCAC
AlwNI 814 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 414 GTGCAC G^TGCAC
Asp700I 904 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 414 GTGCAC G[AT]GC[AT]^C
BalI 210 TGGCCA TGG^CCA
BbeI 92 GGCGCC GGCGC^C
BbvCI 811 CCTCAGC CCTCAGC(-5/-2)
BmyI 414 GTGCAC G[AGT]GC[ACT]^C
BsaAI 256 TACGTG [CT]AC^GT[GA]
BsaHI 92 GGCGCC G[GA]^CG[CT]C
BsaI 440 GAGACC GGTCTC(1/5)
BsaWI 221 TCCGGA [AT]^CCGG[AT]
BseAI 221 TCCGGA T^CCGGA
BsiHKAI 414 GTGCAC G[AT]GC[AT]^C
BsmFI 74 GGGAC GGGAC(10/14)
Bsp1286I 414 GTGCAC G[AGT]GC[ACT]^C
BspEI 221 TCCGGA T^CCGGA
BspMI 1251 GCAGGT ACCTGC(4/8)
BsrFI 154 ACCGGC [GA]^CCGG[CT]
BssSI 1205 CTCGTG CACGAG(-5/-1)
BstAPI 1195 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 130 TTCGAA TT^CGAA
Cfr10I 154 ACCGGC [GA]^CCGG[CT]
DraII 74 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 210 TGGCCA [CT]^GGCC[GA]
EcoO109I 74 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1351 CCAAGG C^C[AT][AT]GG
HgaI 98 GCGTC GACGC(5/10)
Hin1I 92 GGCGCC G[GA]^CG[CT]C
HindIII 710 AAGCTT A^AGCTT
KasI 92 GGCGCC G^GCGCC
KspI 202 CCGCGG CCGC^GG
MluNI 210 TGGCCA TGG^CCA
MroI 221 TCCGGA T^CCGGA
MscI 210 TGGCCA TGG^CCA
MspA1I 202 CCGCGG C[AC]G^C[GT]G
NarI 92 GGCGCC GG^CGCC
NspV 130 TTCGAA TT^CGAA
PpuMI 74 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1246 CTGCAG CTGCA^G
SacII 202 CCGCGG CCGC^GG
SapI 48 GAAGAGC GCTCTTC(1/4)
SfoI 92 GGCGCC GGC^GCC
SfuI 130 TTCGAA TT^CGAA
SmlI 1298 CTCAAG C^T[CT][GA]AG
StuI 694 AGGCCT AGG^CCT
StyI 1351 CCAAGG C^C[AT][AT]GG
XmnI 904 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1039 GTAGAC GT^[AC][GT]AC
1333 GTAGAC
AcsI 849 GAATTC [GA]^AATT[CT]
894 GAATTC
AfaI 63 GTAC GT^AC
505 GTAC
ApoI 849 GAATTC [GA]^AATT[CT]
894 GAATTC
BamHI 736 GGATCC G^GATCC
790 GGATCC
BfaI 229 CTAG C^TAG
1279 CTAG
BpmI 263 CTGGAG CTGGAG(16/14)
941 CTGGAG
BssKI 17 CCAGG ^CC[ACGT]GG
270 CCAGG
BstEII 150 GGTGACC G^GT[ACGT]ACC
1111 GGTGACC
BstF5I 105 CATCC GGATG(2/0)
1074 GGATG
BstNI 17 CCAGG CC^[AT]GG
270 CCAGG
BstPI 150 GGTGACC G^GT[ACGT]ACC
1111 GGTGACC
CfoI 14 GCGC GCG^C
93 GCGC
DsaI 202 CCGCGG C^C[GA][CT]GG
1421 CCGTGG
EcoO65I 150 GGTGACC G^GT[ACGT]ACC
1111 GGTGACC
EcoRI 849 GAATTC G^AATTC
894 GAATTC
EcoRII 17 CCAGG ^CC[AT]GG
270 CCAGG
FokI 105 CATCC GGATG(9/13)
1074 GGATG
HaeII 13 AGCGCC [GA]GCGC^[CT]
92 GGCGCC
HapII 155 CCGG C^CGG
222 CCGG
HhaI 14 GCGC GCG^C
93 GCGC
HinP1I 14 GCGC G^CGC
93 GCGC
HincII 681 GTCAAC GT[CT]^[GA]AC
780 GTCAAC
HindII 681 GTCAAC GT[CT]^[GA]AC
780 GTCAAC
HpaII 155 CCGG C^CGG
222 CCGG
MaeI 229 CTAG C^TAG
1279 CTAG
MspI 155 CCGG C^CGG
222 CCGG
MvaI 17 CCAGG CC^[AT]GG
270 CCAGG
NlaIII 109 CATG CATG^
502 CATG
RsaI 63 GTAC GT^AC
505 GTAC
ScrFI 17 CCAGG CC^[ACGT]GG
270 CCAGG
SfaNI 1075 GATGC GCATC(5/9)
1147 GATGC
SfcI 1142 CTACAG C^T[GA][CT]AG
1246 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BanI BbsI BbvI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsrBI
BsrI BstUI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoNI FauI
Fnu4HI HaeIII HinfI HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MvnI MwoI
NdeII NlaIV Sau3AI Sau96I TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII