Restriction map for
pF1KA1168
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# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AflII AgeI Aor51HI
AscI Asp718I AviII AvrII BbeI
BbrPI BfrI BlnI BsaAI BsaBI
BsiWI BsmBI BspDI BssHII BssSI
Bst1107I BstZ17I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I FseI
FspI HindIII KasI KpnI MamI
MluI NaeI NarI NgoMIV NheI
NotI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SalI ScaI SfiI SfoI SgrAI
SnaBI SpeI SphI SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 3206 TCCGGA T^CCGGA
AclI 2246 AACGTT AA^CGTT
AhdI 1829 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 999 GTGCAC G^TGCAC
ApaI 3402 GGGCCC GGGCC^C
ApaLI 999 GTGCAC G^TGCAC
AseI 868 ATTAAT AT^TAAT
AsnI 868 ATTAAT AT^TAAT
Asp700I 52 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1829 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 2289 GTATCC GTATCC(6/5)
BlpI 3517 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3517 GCTGAGC GC^T[ACGT]AGC
BsaI 958 GAGACC GGTCTC(1/5)
BseAI 3206 TCCGGA T^CCGGA
BsiEI 3687 CGACCG CG[GA][CT]^CG
BspEI 3206 TCCGGA T^CCGGA
BspHI 365 TCATGA T^CATGA
BspLU11I 497 ACATGT A^CATGT
BstBI 1538 TTCGAA TT^CGAA
BstXI 632 CCATGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3517 GCTGAGC GC^T[ACGT]AGC
DraI 2116 TTTAAA TTT^AAA
Eam1105I 1829 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1945 GAATTC G^AATTC
EcoRV 1627 GATATC GAT^ATC
HpaI 1959 GTTAAC GTT^AAC
KspI 1289 CCGCGG CCGC^GG
MfeI 2354 CAATTG C^AATTG
MroI 3206 TCCGGA T^CCGGA
MunI 2354 CAATTG C^AATTG
NspV 1538 TTCGAA TT^CGAA
PshBI 868 ATTAAT AT^TAAT
Psp1406I 2246 AACGTT AA^CGTT
RcaI 365 TCATGA T^CATGA
SacII 1289 CCGCGG CCGC^GG
SexAI 3245 ACCTGGT A^CC[AT]GGT
SfuI 1538 TTCGAA TT^CGAA
SmaI 269 CCCGGG CCC^GGG
XcmI 2453 CCATTGTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 269 CCCGGG C^CCGGG
XmaI 269 CCCGGG C^CCGGG
XmnI 52 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 40 enzymes) #
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AccI 2179 GTCTAC GT^[AC][GT]AC
3175 GTCTAC
AcyI 2275 GGCGTC G[GA]^CG[CT]C
3421 GGCGTC
AocI 1809 CCTGAGG CC^T[ACGT]AGG
3060 CCTGAGG
AspI 451 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
718 GACATTGTC
AvaI 269 CCCGGG C^[CT]CG[GA]G
1686 CCCGAG
BamHI 2740 GGATCC G^GATCC
2984 GGATCC
BbvCI 3277 CCTCAGC CCTCAGC(-5/-2)
2149 GCTGAGG
BcgI 1726 CGAAGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
389 GCAAGGCCATCG
BclI 1367 TGATCA T^GATCA
2169 TGATCA
BfaI 1174 CTAG C^TAG
3170 CTAG
BglI 1479 GCCCCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1724 GCCGAAGAGGC
BsaHI 2275 GGCGTC G[GA]^CG[CT]C
3421 GGCGTC
BsaWI 3206 TCCGGA [AT]^CCGG[AT]
3689 ACCGGA
BsgI 534 GTGCAG GTGCAG(16/14)
3628 GTGCAG
BsmI 147 GAATGC GAATGC(1/-1)
3368 GCATTC
BsoBI 269 CCCGGG C^[CT]CG[GA]G
1686 CCCGAG
BsrFI 2486 ACCGGC [GA]^CCGG[CT]
3455 ACCGGC
BstAPI 906 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2496 GCATCGGCTGC
BstEII 354 GGTCACC G^GT[ACGT]ACC
3304 GGTGACC
BstPI 354 GGTCACC G^GT[ACGT]ACC
3304 GGTGACC
Bsu36I 1809 CCTGAGG CC^T[ACGT]AGG
3060 CCTGAGG
Cfr10I 2486 ACCGGC [GA]^CCGG[CT]
3455 ACCGGC
DraIII 660 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2560 CACAATGTG
Eco81I 1809 CCTGAGG CC^T[ACGT]AGG
3060 CCTGAGG
EcoO65I 354 GGTCACC G^GT[ACGT]ACC
3304 GGTGACC
EcoT22I 81 ATGCAT ATGCA^T
149 ATGCAT
FbaI 1367 TGATCA T^GATCA
2169 TGATCA
Hin1I 2275 GGCGTC G[GA]^CG[CT]C
3421 GGCGTC
HincII 724 GTCAAC GT[CT]^[GA]AC
1959 GTTAAC
HindII 724 GTCAAC GT[CT]^[GA]AC
1959 GTTAAC
MaeI 1174 CTAG C^TAG
3170 CTAG
NcoI 1964 CCATGG C^CATGG
2219 CCATGG
NdeI 781 CATATG CA^TATG
2515 CATATG
NruI 1158 TCGCGA TCG^CGA
3297 TCGCGA
NsiI 81 ATGCAT ATGCA^T
149 ATGCAT
PflFI 451 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
718 GACATTGTC
SbfI 1377 CCTGCAGG CCTGCA^GG
3381 CCTGCAGG
SmlI 790 CTCAAG C^T[CT][GA]AG
1593 CTTGAG
Sse8387I 1377 CCTGCAGG CCTGCA^GG
3381 CCTGCAGG
Tth111I 451 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
718 GACATTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BcnI BglII BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII