Restriction map for
pF1KA1189
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AgeI AhdI AlwI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstUI BstXI BstZ17I Cac8I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I FauI FbaI FseI FspI
HaeII HaeIII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MslI MunI
MvnI MwoI NaeI NarI NciI
NdeI NgoMIV NheI NlaIV NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 506 ACATGT A^C[GA][CT]GT
Alw44I 152 GTGCAC G^TGCAC
AocI 728 CCTTAGG CC^T[ACGT]AGG
ApaLI 152 GTGCAC G^TGCAC
AspHI 152 GTGCAC G[AT]GC[AT]^C
BbsI 383 GAAGAC GAAGAC(2/6)
BglII 285 AGATCT A^GATCT
BmyI 152 GTGCAC G[AGT]GC[ACT]^C
BpmI 750 CTCCAG CTGGAG(16/14)
BpuAI 383 GAAGAC GAAGAC(2/6)
BseRI 529 GAGGAG GAGGAG(10/8)
BsiHKAI 152 GTGCAC G[AT]GC[AT]^C
Bsp1286I 152 GTGCAC G[AGT]GC[ACT]^C
BspLU11I 506 ACATGT A^CATGT
BstBI 485 TTCGAA TT^CGAA
BstEII 451 GGTCACC G^GT[ACGT]ACC
BstPI 451 GGTCACC G^GT[ACGT]ACC
BstYI 285 AGATCT [GA]^GATC[CT]
Bsu36I 728 CCTTAGG CC^T[ACGT]AGG
DpnII 286 GATC ^GATC
DraI 646 TTTAAA TTT^AAA
DsaI 218 CCATGG C^C[GA][CT]GG
Eco81I 728 CCTTAGG CC^T[ACGT]AGG
EcoO65I 451 GGTCACC G^GT[ACGT]ACC
EcoRI 829 GAATTC G^AATTC
EcoRV 745 GATATC GAT^ATC
EcoT14I 218 CCATGG C^C[AT][AT]GG
EcoT22I 844 ATGCAT ATGCA^T
HapII 13 CCGG C^CGG
HincII 65 GTCAAC GT[CT]^[GA]AC
HindII 65 GTCAAC GT[CT]^[GA]AC
HpaII 13 CCGG C^CGG
MboI 286 GATC ^GATC
MflI 285 AGATCT [GA]^GATC[CT]
MspA1I 461 CAGCTG C[AC]G^C[GT]G
MspI 13 CCGG C^CGG
NcoI 218 CCATGG C^CATGG
NdeII 286 GATC ^GATC
NsiI 844 ATGCAT ATGCA^T
NspV 485 TTCGAA TT^CGAA
PvuII 461 CAGCTG CAG^CTG
Sau3AI 286 GATC ^GATC
SfcI 126 CTACAG C^T[GA][CT]AG
SfuI 485 TTCGAA TT^CGAA
SpeI 304 ACTAGT A^CTAGT
StyI 218 CCATGG C^C[AT][AT]GG
TfiI 351 GATTC G^A[AT]TC
XhoII 285 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 613 GAATTT [GA]^AATT[CT]
829 GAATTC
ApoI 613 GAATTT [GA]^AATT[CT]
829 GAATTC
BfaI 305 CTAG C^TAG
494 CTAG
BsaJI 26 CCCAGG C^C[ACGT][ACGT]GG
218 CCATGG
BsrI 140 CCAGT ACTGG(1/-1)
692 CCAGT
BstF5I 215 CATCC GGATG(2/0)
546 GGATG
FokI 215 CATCC GGATG(9/13)
546 GGATG
MaeI 305 CTAG C^TAG
494 CTAG
MseI 617 TTAA T^TAA
647 TTAA
NspI 200 ACATGC [GA]CATG^[CT]
506 ACATGT
PleI 673 GACTC GAGTC(4/5)
835 GAGTC
Tru9I 617 TTAA T^TAA
647 TTAA
Tsp45I 452 GTCAC ^GT[GC]AC
706 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI BbvI BsiYI BslI BssKI
BstNI DdeI EarI EcoRII Fnu4HI
HinfI HphI ItaI Ksp632I MaeIII
MboII MnlI MvaI NlaIII ScrFI
SfaNI TaqI TseI Tsp509I TspRI
TthHB8I