Restriction map for
pF1KA1198
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaII AvrII
BanII BbvCI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I FbaI
FseI HgaI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MroI MspA1I MunI MvnI MwoI
NaeI NciI NdeI NgoMIV NheI
NotI NruI PacI PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI Sau96I SbfI ScaI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 797 GGCGCC G[GA]^CG[CT]C
AvaI 43 CTCGAG C^[CT]CG[GA]G
AviII 2 TGCGCA TGC^GCA
BalI 228 TGGCCA TGG^CCA
BamHI 222 GGATCC G^GATCC
BanI 797 GGCGCC G^G[CT][GA]CC
BbeI 797 GGCGCC GGCGC^C
BbvI 614 GCAGC GCAGC(8/12)
BcgI 683 GCAAAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 526 AGATCT A^GATCT
BsaHI 797 GGCGCC G[GA]^CG[CT]C
BsaI 504 GGTCTC GGTCTC(1/5)
BsaWI 1069 ACCGGA [AT]^CCGG[AT]
BsmFI 950 GTCCC GGGAC(10/14)
BsoBI 43 CTCGAG C^[CT]CG[GA]G
BspHI 969 TCATGA T^CATGA
BspLU11I 1087 ACATGT A^CATGT
BsrDI 927 GCAATG GCAATG(2/0)
BstF5I 180 GGATG GGATG(2/0)
Cac8I 258 GCGGGC GC[ACGT]^[ACGT]GC
DraI 1455 TTTAAA TTT^AAA
DraIII 368 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 135 CCATGG C^C[GA][CT]GG
EaeI 228 TGGCCA [CT]^GGCC[GA]
EarI 791 CTCTTC CTCTTC(1/4)
EcoRV 779 GATATC GAT^ATC
EcoT14I 135 CCATGG C^C[AT][AT]GG
EcoT22I 496 ATGCAT ATGCA^T
FauI 258 GCGGG CCCGC(4/6)
FokI 180 GGATG GGATG(9/13)
FspI 2 TGCGCA TGC^GCA
HaeII 797 GGCGCC [GA]GCGC^[CT]
HapII 1070 CCGG C^CGG
Hin1I 797 GGCGCC G[GA]^CG[CT]C
HindIII 1442 AAGCTT A^AGCTT
HpaII 1070 CCGG C^CGG
KasI 797 GGCGCC G^GCGCC
Ksp632I 791 CTCTTC CTCTTC(1/4)
MluNI 228 TGGCCA TGG^CCA
MscI 228 TGGCCA TGG^CCA
MspI 1070 CCGG C^CGG
NarI 797 GGCGCC GG^CGCC
NcoI 135 CCATGG C^CATGG
NsiI 496 ATGCAT ATGCA^T
NspI 1087 ACATGT [GA]CATG^[CT]
PaeR7I 43 CTCGAG C^TCGAG
PstI 471 CTGCAG CTGCA^G
RcaI 969 TCATGA T^CATGA
SapI 790 GCTCTTC GCTCTTC(1/4)
SfoI 797 GGCGCC GGC^GCC
StyI 135 CCATGG C^C[AT][AT]GG
TseI 614 GCAGC G^C[AT]GC
XhoI 43 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1511 GTAGAC GT^[AC][GT]AC
1574 GTAGAC
AciI 84 CCGC CCGC(-3/-1)
258 GCGG
AfaI 957 GTAC GT^AC
1471 GTAC
AflIII 1053 ACACGT A^C[GA][CT]GT
1087 ACATGT
AluI 616 AGCT AG^CT
1443 AGCT
Alw44I 1135 GTGCAC G^TGCAC
1555 GTGCAC
AlwI 223 GATCC GGATC(4/5)
222 GGATC
ApaLI 1135 GTGCAC G^TGCAC
1555 GTGCAC
AspHI 1135 GTGCAC G[AT]GC[AT]^C
1555 GTGCAC
BbrPI 1054 CACGTG CAC^GTG
1552 CACGTG
BbsI 490 GTCTTC GAAGAC(2/6)
1360 GTCTTC
BmyI 1135 GTGCAC G[AGT]GC[ACT]^C
1555 GTGCAC
BpuAI 490 GTCTTC GAAGAC(2/6)
1360 GTCTTC
BseRI 46 GAGGAG GAGGAG(10/8)
205 GAGGAG
BsiHKAI 1135 GTGCAC G[AT]GC[AT]^C
1555 GTGCAC
BsmI 1339 GAATGC GAATGC(1/-1)
772 GCATTC
Bsp1286I 1135 GTGCAC G[AGT]GC[ACT]^C
1555 GTGCAC
BstYI 222 GGATCC [GA]^GATC[CT]
526 AGATCT
CfoI 3 GCGC GCG^C
798 GCGC
DdeI 19 CTCAG C^T[ACGT]AG
346 CTAAG
Fnu4HI 83 GCCGC GC^[ACGT]GC
614 GCAGC
HhaI 3 GCGC GCG^C
798 GCGC
HinP1I 3 GCGC G^CGC
798 GCGC
HphI 197 TCACC GGTGA(8/7)
1377 TCACC
ItaI 83 GCCGC GC^[ACGT]GC
614 GCAGC
MflI 222 GGATCC [GA]^GATC[CT]
526 AGATCT
NlaIV 222 GGATCC GG[ACGT]^[ACGT]CC
797 GGCGCC
PmaCI 1054 CACGTG CAC^GTG
1552 CACGTG
PmlI 1054 CACGTG CAC^GTG
1552 CACGTG
RsaI 957 GTAC GT^AC
1471 GTAC
SfcI 243 CTACAG C^T[GA][CT]AG
471 CTGCAG
TfiI 417 GATTC G^A[AT]TC
1301 GAATC
XhoII 222 GGATCC [GA]^GATC[CT]
526 AGATCT
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI ApoI Asp700I BfaI BpmI
BsaAI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstBI BstNI DpnII
EcoRI EcoRII HaeIII HinfI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
NspV PleI Sau3AI ScrFI SfaNI
SfuI SmlI TaqI Tru9I Tsp45I
Tsp509I TspRI TthHB8I XmnI