Restriction map for
pF1KA1204
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BciVI BclI BfrI
BlnI BsaAI BsaBI BsiEI BsiWI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BssSI Bst1107I BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfiI
SnaBI SpeI SphI SspBI Tth111I
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# ONE CUT ENZYMES ( 34 enzymes) #
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AgeI 1108 ACCGGT A^CCGGT
Alw44I 656 GTGCAC G^TGCAC
ApaLI 656 GTGCAC G^TGCAC
BglI 2248 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 16 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 16 GCTAAGC GC^T[ACGT]AGC
BsmBI 2610 CGTCTC CGTCTC(1/5)
BspHI 695 TCATGA T^CATGA
BstBI 1368 TTCGAA TT^CGAA
BstEII 2806 GGTCACC G^GT[ACGT]ACC
BstPI 2806 GGTCACC G^GT[ACGT]ACC
CelII 16 GCTAAGC GC^T[ACGT]AGC
DraI 1794 TTTAAA TTT^AAA
DrdI 3415 GACCCAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2806 GGTCACC G^GT[ACGT]ACC
EcoRI 2946 GAATTC G^AATTC
HgaI 2773 GACGC GACGC(5/10)
HincII 2379 GTCAAC GT[CT]^[GA]AC
HindII 2379 GTCAAC GT[CT]^[GA]AC
NcoI 836 CCATGG C^CATGG
NspV 1368 TTCGAA TT^CGAA
PinAI 1108 ACCGGT A^CCGGT
RcaI 695 TCATGA T^CATGA
SbfI 1875 CCTGCAGG CCTGCA^GG
SfuI 1368 TTCGAA TT^CGAA
SgrAI 1107 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1213 CCCGGG CCC^GGG
Sse8387I 1875 CCTGCAGG CCTGCA^GG
SspI 918 AATATT AAT^ATT
SwaI 1793 ATTTAAAT ATTT^AAAT
XbaI 2862 TCTAGA T^CTAGA
XcmI 3418 CCAAAGGTCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1213 CCCGGG C^CCGGG
XmaI 1213 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccIII 1320 TCCGGA T^CCGGA
1821 TCCGGA
AcyI 522 GGCGCC G[GA]^CG[CT]C
1741 GGCGCC
AocI 534 CCTCAGG CC^T[ACGT]AGG
3009 CCTGAGG
ApaI 2071 GGGCCC GGGCC^C
3286 GGGCCC
Asp700I 1296 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2266 GAAATAGTTC
BamHI 1317 GGATCC G^GATCC
2160 GGATCC
BbeI 522 GGCGCC GGCGC^C
1741 GGCGCC
BbvCI 4282 CCTCAGC CCTCAGC(-5/-2)
1219 GCTGAGG
BcgI 1461 CGAGCCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1538 CGAAGGAGCTGC
BsaHI 522 GGCGCC G[GA]^CG[CT]C
1741 GGCGCC
BseAI 1320 TCCGGA T^CCGGA
1821 TCCGGA
BspEI 1320 TCCGGA T^CCGGA
1821 TCCGGA
BsrBI 1483 CCGCTC CCGCTC(-3/-3)
1593 GAGCGG
BstAPI 1517 GCACGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2226 GCAGGGCCTGC
BstXI 731 CCATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2135 CCACCCCTCTGG
Bsu36I 534 CCTCAGG CC^T[ACGT]AGG
3009 CCTGAGG
DsaI 836 CCATGG C^C[GA][CT]GG
2876 CCACGG
Eco81I 534 CCTCAGG CC^T[ACGT]AGG
3009 CCTGAGG
FauI 764 CCCGC CCCGC(4/6)
3253 CCCGC
Hin1I 522 GGCGCC G[GA]^CG[CT]C
1741 GGCGCC
KasI 522 GGCGCC G^GCGCC
1741 GGCGCC
MaeII 107 ACGT A^CGT
933 ACGT
MroI 1320 TCCGGA T^CCGGA
1821 TCCGGA
MslI 1792 CATTTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3426 CACATGGATG
NarI 522 GGCGCC GG^CGCC
1741 GGCGCC
PaeR7I 2056 CTCGAG C^TCGAG
2899 CTCGAG
SapI 114 GAAGAGC GCTCTTC(1/4)
699 GAAGAGC
SfoI 522 GGCGCC GGC^GCC
1741 GGCGCC
StuI 1722 AGGCCT AGG^CCT
3307 AGGCCT
XhoI 2056 CTCGAG C^TCGAG
2899 CTCGAG
XmnI 1296 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2266 GAAATAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII