Restriction map for
pF1KA1237
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AflIII AgeI AlwNI
Aor51HI AscI AviII BclI BsaBI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspLU11I BsrDI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI Eco47III EcoO65I EcoRV
FbaI FseI FspI HpaI MamI
MfeI MluI MroI MunI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SnaBI SphI Sse8387I SspI StuI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 3464 GGTACC G^GTACC
AccI 2412 GTCTAC GT^[AC][GT]AC
AflII 2635 CTTAAG C^TTAAG
AhdI 1041 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 842 CCTCAGG CC^T[ACGT]AGG
AseI 2221 ATTAAT AT^TAAT
AsnI 2221 ATTAAT AT^TAAT
Asp700I 619 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3464 GGTACC G^GTACC
AspEI 1041 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1323 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4092 CCTAGG C^CTAGG
BalI 337 TGGCCA TGG^CCA
BamHI 3436 GGATCC G^GATCC
BbrPI 1574 CACGTG CAC^GTG
BbvCI 3775 GCTGAGG CCTCAGC(-5/-2)
BfrI 2635 CTTAAG C^TTAAG
BglII 852 AGATCT A^GATCT
BlnI 4092 CCTAGG C^CTAGG
BsiEI 291 CGGCCG CG[GA][CT]^CG
Bsp1407I 1479 TGTACA T^GTACA
BspHI 2600 TCATGA T^CATGA
BsrFI 75 GCCGGC [GA]^CCGG[CT]
BsrGI 1479 TGTACA T^GTACA
BstXI 3665 CCAACGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 842 CCTCAGG CC^T[ACGT]AGG
Cfr10I 75 GCCGGC [GA]^CCGG[CT]
DraI 3517 TTTAAA TTT^AAA
EagI 291 CGGCCG C^GGCCG
Eam1105I 1041 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 291 CGGCCG C^GGCCG
Eco52I 291 CGGCCG C^GGCCG
Eco81I 842 CCTCAGG CC^T[ACGT]AGG
EcoNI 256 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KpnI 3464 GGTACC GGTAC^C
MluNI 337 TGGCCA TGG^CCA
MscI 337 TGGCCA TGG^CCA
NaeI 75 GCCGGC GCC^GGC
NcoI 2434 CCATGG C^CATGG
NdeI 4184 CATATG CA^TATG
NgoMIV 75 GCCGGC G^CCGGC
PflFI 1323 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1574 CACGTG CAC^GTG
PmlI 1574 CACGTG CAC^GTG
PshAI 1220 GACTTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2221 ATTAAT AT^TAAT
RcaI 2600 TCATGA T^CATGA
SgrAI 74 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 2635 CTTAAG C^T[CT][GA]AG
SpeI 2967 ACTAGT A^CTAGT
SspBI 1479 TGTACA T^GTACA
Tth111I 1323 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 850 CCAGATCTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 619 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AclI 1583 AACGTT AA^CGTT
3597 AACGTT
AcyI 135 GGCGCC G[GA]^CG[CT]C
265 GGCGCC
Alw44I 745 GTGCAC G^TGCAC
1880 GTGCAC
ApaLI 745 GTGCAC G^TGCAC
1880 GTGCAC
BbeI 135 GGCGCC GGCGC^C
265 GGCGCC
BcgI 1022 CGAGAACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1135 GCAGTGGAATCG
BciVI 3916 GGATAC GTATCC(6/5)
2464 GTATCC
BglI 3345 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4001 GCCCATTTGGC
BlpI 126 GCTGAGC GC^T[ACGT]AGC
2158 GCTCAGC
Bpu1102I 126 GCTGAGC GC^T[ACGT]AGC
2158 GCTCAGC
BsaAI 1574 CACGTG [CT]AC^GT[GA]
1825 CACGTA
BsaHI 135 GGCGCC G[GA]^CG[CT]C
265 GGCGCC
BsaI 1854 GGTCTC GGTCTC(1/5)
4018 GGTCTC
BssHII 12 GCGCGC G^CGCGC
122 GCGCGC
BstAPI 3824 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3992 GCATGAGTTGC
CelII 126 GCTGAGC GC^T[ACGT]AGC
2158 GCTCAGC
DraIII 1571 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
1622 CACTAGGTG
EcoRI 1186 GAATTC G^AATTC
1560 GAATTC
Hin1I 135 GGCGCC G[GA]^CG[CT]C
265 GGCGCC
KasI 135 GGCGCC G^GCGCC
265 GGCGCC
KspI 219 CCGCGG CCGC^GG
4067 CCGCGG
NarI 135 GGCGCC GG^CGCC
265 GGCGCC
NspI 990 GCATGT [GA]CATG^[CT]
3955 GCATGT
Psp1406I 1583 AACGTT AA^CGTT
3597 AACGTT
SacI 960 GAGCTC GAGCT^C
2049 GAGCTC
SacII 219 CCGCGG CCGC^GG
4067 CCGCGG
SapI 447 GAAGAGC GCTCTTC(1/4)
524 GCTCTTC
SfoI 135 GGCGCC GGC^GCC
265 GGCGCC
SmaI 85 CCCGGG CCC^GGG
209 CCCGGG
XmaCI 85 CCCGGG C^CCGGG
209 CCCGGG
XmaI 85 CCCGGG C^CCGGG
209 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApaI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII