Restriction map for
pF1KA1248
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AflIII AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII CfoI ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MflI MluI
MluNI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 57 GACGCC G[GA]^CG[CT]C
AgeI 983 ACCGGT A^CCGGT
AlwNI 196 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1039 GGGCCC GGGCC^C
AspHI 574 GAGCTC G[AT]GC[AT]^C
AvaI 1044 CCCGGG C^[CT]CG[GA]G
BanI 124 GGCACC G^G[CT][GA]CC
BglI 1041 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 398 CTGGAG CTGGAG(16/14)
BsaHI 57 GACGCC G[GA]^CG[CT]C
BseRI 31 GAGGAG GAGGAG(10/8)
BsiHKAI 574 GAGCTC G[AT]GC[AT]^C
BsoBI 1044 CCCGGG C^[CT]CG[GA]G
BspHI 756 TCATGA T^CATGA
BsrBI 990 CCGCTC CCGCTC(-3/-3)
BsrFI 983 ACCGGT [GA]^CCGG[CT]
BstAPI 12 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 983 ACCGGT [GA]^CCGG[CT]
DrdI 924 GACTCGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1055 CCATGG C^C[GA][CT]GG
EaeI 68 CGGCCA [CT]^GGCC[GA]
Hin1I 57 GACGCC G[GA]^CG[CT]C
MseI 428 TTAA T^TAA
MspA1I 844 CAGCTG C[AC]G^C[GT]G
NcoI 1055 CCATGG C^CATGG
PinAI 983 ACCGGT A^CCGGT
PvuII 844 CAGCTG CAG^CTG
RcaI 756 TCATGA T^CATGA
SacI 574 GAGCTC GAGCT^C
SapI 571 GAAGAGC GCTCTTC(1/4)
ScaI 881 AGTACT AGT^ACT
SmaI 1044 CCCGGG CCC^GGG
SmlI 814 CTCAAG C^T[CT][GA]AG
TaqI 212 TCGA T^CGA
Tru9I 428 TTAA T^TAA
Tsp45I 106 GTCAC ^GT[GC]AC
TthHB8I 212 TCGA T^CGA
XcmI 482 CCAAGGGTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1044 CCCGGG C^CCGGG
XmaI 1044 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 537 TCCGGA T^CCGGA
829 TCCGGA
AcsI 363 AAATTT [GA]^AATT[CT]
585 AAATTC
AocI 61 CCTGAGG CC^T[ACGT]AGG
714 CCTCAGG
ApoI 363 AAATTT [GA]^AATT[CT]
585 AAATTC
Asp700I 226 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1025 GAACTCTTTC
BseAI 537 TCCGGA T^CCGGA
829 TCCGGA
BsgI 19 GTGCAG GTGCAG(16/14)
962 GTGCAG
BspEI 537 TCCGGA T^CCGGA
829 TCCGGA
Bsu36I 61 CCTGAGG CC^T[ACGT]AGG
714 CCTCAGG
Cac8I 327 GCTTGC GC[ACGT]^[ACGT]GC
860 GCAAGC
DraII 1038 GGGGCCC [GA]G^G[ACGT]CC[CT]
1039 GGGCCCC
DraIII 79 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
163 CACGATGTG
Eco81I 61 CCTGAGG CC^T[ACGT]AGG
714 CCTCAGG
EcoO109I 1038 GGGGCCC [GA]G^G[ACGT]CC[CT]
1039 GGGCCCC
EcoRV 302 GATATC GAT^ATC
706 GATATC
FauI 989 CCCGC CCCGC(4/6)
246 GCGGG
HgaI 57 GACGC GACGC(5/10)
347 GCGTC
MaeIII 106 GTCAC ^GT[ACGT]AC
779 GTAAC
MroI 537 TCCGGA T^CCGGA
829 TCCGGA
MslI 253 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
757 CATGAAGATG
NspI 218 ACATGC [GA]CATG^[CT]
624 ACATGC
PflMI 162 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
482 CCAAGGGTTGG
PstI 10 CTGCAG CTGCA^G
352 CTGCAG
SfcI 10 CTGCAG C^T[GA][CT]AG
352 CTGCAG
StuI 519 AGGCCT AGG^CCT
872 AGGCCT
Van91I 162 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
482 CCAAGGGTTGG
XmnI 226 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1025 GAACTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BcnI BmyI BsaI
BsaJI BsaWI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp509I
TspRI