Restriction map for
pF1KA1332
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BbeI BcgI BclI BfrI BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsgI BsiHKAI BsiWI BspDI BspEI
BspLU11I BspMI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI FspI Hin1I HindIII KasI
KpnI KspI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 578 CGCG CG^CG
AlwNI 1811 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 353 AGCGCT AGC^GCT
ApaI 1004 GGGCCC GGGCC^C
BbrPI 1624 CACGTG CAC^GTG
BbvCI 112 GCTGAGG CCTCAGC(-5/-2)
BciVI 1417 GGATAC GTATCC(6/5)
BglI 1856 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1233 GAGACC GGTCTC(1/5)
BsiEI 1215 CGGCCG CG[GA][CT]^CG
BsmI 951 GCATTC GAATGC(1/-1)
BspHI 278 TCATGA T^CATGA
BsrBI 1167 CCGCTC CCGCTC(-3/-3)
BstUI 578 CGCG CG^CG
CfoI 354 GCGC GCG^C
EagI 1215 CGGCCG C^GGCCG
EclXI 1215 CGGCCG C^GGCCG
Eco47III 353 AGCGCT AGC^GCT
Eco52I 1215 CGGCCG C^GGCCG
EcoT22I 949 ATGCAT ATGCA^T
HaeII 353 AGCGCT [GA]GCGC^[CT]
HhaI 354 GCGC GCG^C
HinP1I 354 GCGC G^CGC
HincII 1210 GTTAAC GT[CT]^[GA]AC
HindII 1210 GTTAAC GT[CT]^[GA]AC
HpaI 1210 GTTAAC GTT^AAC
MaeIII 670 GTGAC ^GT[ACGT]AC
MvnI 578 CGCG CG^CG
NsiI 949 ATGCAT ATGCA^T
PaeR7I 845 CTCGAG C^TCGAG
PmaCI 1624 CACGTG CAC^GTG
PmlI 1624 CACGTG CAC^GTG
PstI 1721 CTGCAG CTGCA^G
RcaI 278 TCATGA T^CATGA
SfcI 1721 CTGCAG C^T[GA][CT]AG
SmaI 1268 CCCGGG CCC^GGG
SphI 370 GCATGC GCATG^C
TfiI 614 GATTC G^A[AT]TC
Tsp45I 670 GTGAC ^GT[GC]AC
XhoI 845 CTCGAG C^TCGAG
XmaCI 1268 CCCGGG C^CCGGG
XmaI 1268 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BanI 1019 GGTGCC G^G[CT][GA]CC
1660 GGTGCC
BbsI 1057 GTCTTC GAAGAC(2/6)
1746 GTCTTC
BglII 1479 AGATCT A^GATCT
1500 AGATCT
BpuAI 1057 GTCTTC GAAGAC(2/6)
1746 GTCTTC
BsaAI 1624 CACGTG [CT]AC^GT[GA]
1939 TACGTG
BsaBI 382 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1487 GATTAGGATC
BsmBI 1650 CGTCTC CGTCTC(1/5)
1298 GAGACG
Bsp1407I 542 TGTACA T^GTACA
1377 TGTACA
BsrGI 542 TGTACA T^GTACA
1377 TGTACA
BstXI 1009 CCAGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1821 CCAAGGAGATGG
EcoNI 1804 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1816 CCTGCCCAAGG
FauI 1522 CCCGC CCCGC(4/6)
1974 GCGGG
HgaI 576 GACGC GACGC(5/10)
1668 GCGTC
MaeII 1625 ACGT A^CGT
1940 ACGT
MamI 382 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1487 GATTAGGATC
MslI 36 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1929 CATGCAGATG
NspI 370 GCATGC [GA]CATG^[CT]
947 ACATGC
PpuMI 502 GGGTCCT [GA]G^G[AT]CC[CT]
782 GGGTCCT
PvuII 426 CAGCTG CAG^CTG
1346 CAGCTG
SapI 994 GAAGAGC GCTCTTC(1/4)
1358 GCTCTTC
ScaI 1396 AGTACT AGT^ACT
2105 AGTACT
SspBI 542 TGTACA T^GTACA
1377 TGTACA
TaqI 237 TCGA T^CGA
846 TCGA
Tsp509I 652 AATT ^AATT
1507 AATT
TthHB8I 237 TCGA T^CGA
846 TCGA
XcmI 1065 CCAGGCTCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1541 CCATGGATGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BalI BamHI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI Tru9I TseI
TspRI XhoII